Reviewed, UniProtKB/Swiss-Prot Q5E998 (CATL2_BOVIN)

Names and origin

Protein names

Recommended name:
    Cathepsin L2
    EC=3.4.22.43

Gene names

Name:

CTSL2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cysteine protease. May have an important role in corneal physiology By similarity.
Catalytic activity	The recombinant enzyme hydrolyzes proteins (serum albumin, collagen) and synthetic substrates (Z-Phe-Arg-NHMec > Z-Leu-Arg-NHMec > Z-Val-Arg-NHMec).
Subcellular location	Lysosome Potential.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Propeptide	18 – 113	96	Activation peptide By similarity		PRO_0000238118
	Chain	114 – 334	221	Cathepsin L2		PRO_0000238119
Sites
	Active site	138	1	By similarity
	Active site	276	1	By similarity
	Active site	301	1	By similarity
Amino acid modifications
	Glycosylation	222	1	N-linked (GlcNAc...) Potential
	Disulfide bond	135 ↔ 178		By similarity
	Disulfide bond	169 ↔ 212		By similarity
	Disulfide bond	270 ↔ 323		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5E998-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: ECA2BA83241BDBC9	FASTA	334	37,393
	10 20 30 40 50 60 MNPSFFLTVL CLGVASAAPK LDPNLDAHWH QWKATHRRLY GMNEEEWRRA VWEKNKKIID 70 80 90 100 110 120 LHNQEYSEGK HGFRMAMNAF GDMTNEEFRQ VMNGFQNQKH KKGKLFHEPL LVDVPKSVDW 130 140 150 160 170 180 TKKGYVTPVK NQGQCGSCWA FSATGALEGQ MFRKTGKLVS LSEQNLVDCS RAQGNQGCNG 190 200 210 220 230 240 GLMDNAFQYI KDNGCLDSEE SYPYLATDTN SCNYKPECSA ANDTGFVDIP QREKALMKAV 250 260 270 280 290 300 ATVGPISVAI DAGHTSFQFY KSGIYYDPDC SSKDLDHGVL VVGYGFEGTD SNNNKFWIVK 310 320 330 NSWGPEWGWN GYVKMAKDQN NHCGIATAAS YPTV

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BT021022 mRNA. Translation: AAX09039.1.
IPI	IPI00711962.
UniGene	Bt.3987
3D structure databases
SMR	Q5E998. Positions 21-334.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5E998.
Protein family/group databases
MEROPS	C01.032.
Genome annotation databases
Ensembl	ENSBTAT00000022710; ENSBTAP00000022710; ENSBTAG00000017077; Bos taurus. [Genome view] ENSBTAT00000036427; ENSBTAP00000036285; ENSBTAG00000017077; Bos taurus. [Genome view]
Phylogenomic databases
HOVERGEN	Q5E998.
Enzyme and pathway databases
BRENDA	3.4.22.43. 251.
Family and domain databases
InterPro	IPR000169. Pept_cys_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. IPR013201. Prot_inhib_I29. [Graphical view]
PANTHER	PTHR12411. Peptidase_C1A. 1 hit.
Pfam	PF08246. Inhibitor_I29. 1 hit. PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
ProDom	PD000158. Peptidase_C1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CATL2_BOVIN

Accession

Primary (citable) accession number: Q5E998

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	March 15, 2005
Last modified:	September 1, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)