Q5E985 (HYAL1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hyaluronidase-1
Short name=Hyal-1
EC=3.2.1.35

Alternative name(s):
Hyaluronoglucosaminidase-1

Gene names

Name:

HYAL1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth By similarity.
Catalytic activity	Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.
Subcellular location	Secreted By similarity. Lysosome By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 56 family. Contains 1 EGF-like domain.
Sequence caution	The sequence AAX08792.1 differs from that shown. Reason: Frameshift at position 139.

Ontologies

Keywords
Cellular component	Lysosome Secreted
Domain	EGF-like domain Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hyalurononglucosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	Potential
	Chain	36 – 450	415	Hyaluronidase-1		PRO_0000042623
Regions
	Domain	433 – 444	12	EGF-like
Sites
	Active site	146	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	85	1	N-linked (GlcNAc...) Potential
	Glycosylation	231	1	N-linked (GlcNAc...) Potential
	Glycosylation	365	1	N-linked (GlcNAc...) Potential
	Glycosylation	398	1	N-linked (GlcNAc...) Potential
	Disulfide bond	58 ↔ 348		By similarity
	Disulfide bond	222 ↔ 236		By similarity
	Disulfide bond	373 ↔ 384		By similarity
	Disulfide bond	378 ↔ 433		By similarity
	Disulfide bond	435 ↔ 444		By similarity
Experimental info
	Sequence conflict	333	1	V → L in AAI02474. Ref.2
	Sequence conflict	449	1	M → L in AAI02474. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q5E985 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 576DD38745585AB5	FASTA	450	50,508
	10 20 30 40 50 60 MRPFSLEVSL HLPWAMAAHL LPVCTLFLNL LSMTQGSRDP VVPNQPFTTI WNANTEWCMK 70 80 90 100 110 120 KHGVDVDISI FDVVTNPGQT FRGPNMTIFY SSQLGTYPYY TSAGEPVFGG LPQNASLNAH 130 140 150 160 170 180 LARTFQDILA AMPEPRFSGL AVIDWEAWRP RWAFNWDTKD IYRQRSRALV QKQHPDWLAP 190 200 210 220 230 240 RVEAAAQDQF EGAAEEWMAG TLKLGQALRP QGLWGFYNFP ECYNYDFKSP NYTGRCPLNI 250 260 270 280 290 300 CAQNDQLGWL WGQSRALYPS IYLPAALEGT KKTQMFVQHR VAEAFRVAAG AGDPKLPVLP 310 320 330 340 350 360 YMQLFYDMTN HFLPAEELEH SLGESAAQGA AGVVLWVSWL STSTKESCQA IKEYVDTTLG 370 380 390 400 410 420 PSILNVTSGA RLCSQVLCSG HGRCARRPSY PKARLILNST SFSIKPTPGG GPLTLQGALS 430 440 450 LEDRLRMAVE FECRCYRGWR GTRCEQWGMW

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT020775 mRNA. Translation: AAX08792.1. Frameshift. BT021035 mRNA. Translation: AAX09052.1. BC102473 mRNA. Translation: AAI02474.1.
IPI	IPI00705752.
RefSeq	NP_001017941.1. NM_001017941.1.
UniGene	Bt.4740.
3D structure databases
ProteinModelPortal	Q5E985.
SMR	Q5E985. Positions 38-450.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5E985.
Protein family/group databases
CAZy	GH56. Glycoside Hydrolase Family 56.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	515397.
KEGG	bta:515397.
Organism-specific databases
CTD	3373.
Phylogenomic databases
eggNOG	maNOG06825.
GeneTree	ENSGT00550000074476.
HOVERGEN	HBG052053.
InParanoid	Q5E985.
OrthoDB	EOG4VDPZD.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR006210. EGF-like. IPR017853. Glycoside_hydrolase_SF. IPR018155. Hyaluronidase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01197.
PANTHER	PTHR11769. Glyco_hydro_56. 1 hit.
Pfam	PF01630. Glyco_hydro_56. 1 hit. [Graphical view]
PIRSF	PIRSF038193. Hyaluronidase. 1 hit.
PRINTS	PR00846. GLHYDRLASE56.
SMART	SM00181. EGF. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HYAL1_BOVIN

Accession

Primary (citable) accession number: Q5E985
Secondary accession number(s): Q3T0A8, Q5E9Z4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	March 15, 2005
Last modified:	November 16, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program