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Protein

Eukaryotic translation initiation factor 3 subunit I

Gene

EIF3I

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis

Enzyme and pathway databases

ReactomeiR-BTA-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-BTA-72649 Translation initiation complex formation
R-BTA-72702 Ribosomal scanning and start codon recognition

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit IUniRule annotation
Short name:
eIF3iUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 2UniRule annotation
eIF-3-betaUniRule annotation
eIF3 p36UniRule annotation
Gene namesi
Name:EIF3IUniRule annotation
Synonyms:EIF3S2UniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 2

Organism-specific databases

VGNCiVGNC:28399 EIF3I

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000510351 – 325Eukaryotic translation initiation factor 3 subunit IAdd BLAST325

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei219PhosphothreonineBy similarity1
Modified residuei264N6-acetyllysineBy similarity1
Cross-linki282Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei308PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylated by TGF-beta type II receptor.UniRule annotation

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ5E966
PeptideAtlasiQ5E966
PRIDEiQ5E966

Expressioni

Gene expression databases

BgeeiENSBTAG00000014388

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.UniRule annotation

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019134

Structurei

3D structure databases

ProteinModelPortaliQ5E966
SMRiQ5E966
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati8 – 47WD 1Add BLAST40
Repeati50 – 91WD 2Add BLAST42
Repeati144 – 183WD 3Add BLAST40
Repeati186 – 225WD 4Add BLAST40
Repeati283 – 324WD 5Add BLAST42

Sequence similaritiesi

Belongs to the eIF-3 subunit I family.UniRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0643 Eukaryota
ENOG410XQ3E LUCA
GeneTreeiENSGT00630000089849
HOGENOMiHOG000231322
HOVERGENiHBG000900
InParanoidiQ5E966
KOiK03246
OMAiFETRFWH
OrthoDBiEOG091G0AIZ
TreeFamiTF101515

Family and domain databases

Gene3Di2.130.10.10, 1 hit
HAMAPiMF_03008 eIF3i, 1 hit
InterProiView protein in InterPro
IPR027525 eIF3i
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR19877 PTHR19877, 1 hit
PfamiView protein in Pfam
PF00400 WD40, 3 hits
SMARTiView protein in SMART
SM00320 WD40, 5 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 4 hits
PS50294 WD_REPEATS_REGION, 2 hits

Sequencei

Sequence statusi: Complete.

Q5E966-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPILLQGHE RSITQIKYNR EGDLLFTVAK DPIVNVWYSV NGERLGTYMG
60 70 80 90 100
HTGAVWCVDA DWDTKHVLTG SADNSCRLWD CETGKQLALL KTNSAVRTCG
110 120 130 140 150
FDFGGNIIMF STDKQMGYQC FVSFFDLRDP SQIDSNEPYM RIPCNDSKIT
160 170 180 190 200
SAVWGPLGEC IIAGHEGGEL NQYSAKSGEV LVNVKEHSRQ INDIQLSRDM
210 220 230 240 250
TMFVTASKDN TAKLFDSTTL EHQKTFRTER PVNSAALSPN YDHVVLGGGQ
260 270 280 290 300
EAMDVTTTST RIGKFEARFF HLAFEEEFGR VKGHFGPINS VAFHPDGKSY
310 320
SSGGEDGYVR IHYFDPQYFE FEFEA
Length:325
Mass (Da):36,473
Last modified:March 15, 2005 - v1
Checksum:iDF4A9698F8501EFE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021054 mRNA Translation: AAX09071.1
BC103177 mRNA Translation: AAI03178.1
RefSeqiNP_001015628.1, NM_001015628.1
UniGeneiBt.37139

Genome annotation databases

EnsembliENSBTAT00000019134; ENSBTAP00000019134; ENSBTAG00000014388
GeneIDi521747
KEGGibta:521747

Similar proteinsi

Entry informationi

Entry nameiEIF3I_BOVIN
AccessioniPrimary (citable) accession number: Q5E966
Secondary accession number(s): Q3SZ38
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: March 15, 2005
Last modified: May 23, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. SIMILARITY comments
    Index of protein domains and families

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