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Protein

DnaJ homolog subfamily A member 1

Gene

DNAJA1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Co-chaperone for HSPA8/Hsc70. Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis. Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro). Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi134Zinc 1By similarity1
Metal bindingi137Zinc 1By similarity1
Metal bindingi150Zinc 2By similarity1
Metal bindingi153Zinc 2By similarity1
Metal bindingi177Zinc 2By similarity1
Metal bindingi180Zinc 2By similarity1
Metal bindingi193Zinc 1By similarity1
Metal bindingi196Zinc 1By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri121 – 205CR-typeAdd BLAST85

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily A member 1
Gene namesi
Name:DNAJA1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 8

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002398051 – 394DnaJ homolog subfamily A member 1Add BLAST394
PropeptideiPRO_0000396751395 – 397Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei66N6-acetyllysineBy similarity1
Modified residuei83PhosphoserineBy similarity1
Modified residuei335PhosphoserineBy similarity1
Modified residuei381PhosphotyrosineBy similarity1
Modified residuei394Cysteine methyl esterBy similarity1
Lipidationi394S-farnesyl cysteineBy similarity1

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiQ5E954.
PeptideAtlasiQ5E954.
PRIDEiQ5E954.

Expressioni

Gene expression databases

BgeeiENSBTAG00000016265.
ExpressionAtlasiQ5E954. differential.

Interactioni

Subunit structurei

Identified in a complex with HSPA1B and BAX. Interacts with RNF207.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000021637.

Structurei

3D structure databases

ProteinModelPortaliQ5E954.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 68JAdd BLAST63
Repeati134 – 141CXXCXGXG motif8
Repeati150 – 157CXXCXGXG motif8
Repeati177 – 184CXXCXGXG motif8
Repeati193 – 200CXXCXGXG motif8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi75 – 96Gly-richAdd BLAST22

Sequence similaritiesi

Contains 1 CR-type zinc finger.Curated
Contains 1 J domain.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri121 – 205CR-typeAdd BLAST85

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0712. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00860000133716.
HOGENOMiHOG000226718.
HOVERGENiHBG066727.
InParanoidiQ5E954.
KOiK09502.
OMAiIIEFRVI.
OrthoDBiEOG091G0CAC.
TreeFamiTF105141.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5E954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKETTYYDV LGVKPNATQE ELKKAYRKLA LKYHPDKNPN EGEKFKQISQ
60 70 80 90 100
AYEVLSDAKK RELYDKGGEQ AIKEGGAGGG FGSPMDIFDM FFGGGGRMQR
110 120 130 140 150
ERRGKNVVHQ LTVTLEDLYN GATRKLALQK NVICDKCEGR GGKKGAVECC
160 170 180 190 200
PNCRGTGMQI RIHQIGPGMV QQIQSVCMEC QGHGERISPK DRCKSCNGRK
210 220 230 240 250
IVREKKILEV HIDKGMKDGQ KITFHGEGDQ EPGLEPGDII IVLDQKDHAV
260 270 280 290 300
FTRRGEDLFM CMDIQLVEAL CGFQKPISTL DNRTIVITSH PGQIVKHGDI
310 320 330 340 350
KCVLNEGMPI YRRPYEKGRL IIEFKVNFPE NGFLSPDKLS LLEKLLPERK
360 370 380 390
EVEETDEMDQ VELVDFDPNQ ERRRHYNGEA YEDDEHHPRG GVQCQTS
Length:397
Mass (Da):44,882
Last modified:April 17, 2007 - v2
Checksum:iFDCB27F110B2E12C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti99Q → H in AAX09083 (PubMed:16305752).Curated1
Sequence conflicti181 – 182QG → HC in AAX09083 (PubMed:16305752).Curated2
Sequence conflicti188S → T in AAX09083 (PubMed:16305752).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021066 mRNA. Translation: AAX09083.1.
BC102711 mRNA. Translation: AAI02712.1.
RefSeqiNP_001015637.1. NM_001015637.1.
UniGeneiBt.64693.

Genome annotation databases

EnsembliENSBTAT00000021637; ENSBTAP00000021637; ENSBTAG00000016265.
GeneIDi528862.
KEGGibta:528862.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021066 mRNA. Translation: AAX09083.1.
BC102711 mRNA. Translation: AAI02712.1.
RefSeqiNP_001015637.1. NM_001015637.1.
UniGeneiBt.64693.

3D structure databases

ProteinModelPortaliQ5E954.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000021637.

Proteomic databases

PaxDbiQ5E954.
PeptideAtlasiQ5E954.
PRIDEiQ5E954.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000021637; ENSBTAP00000021637; ENSBTAG00000016265.
GeneIDi528862.
KEGGibta:528862.

Organism-specific databases

CTDi3301.

Phylogenomic databases

eggNOGiKOG0712. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00860000133716.
HOGENOMiHOG000226718.
HOVERGENiHBG066727.
InParanoidiQ5E954.
KOiK09502.
OMAiIIEFRVI.
OrthoDBiEOG091G0CAC.
TreeFamiTF105141.

Gene expression databases

BgeeiENSBTAG00000016265.
ExpressionAtlasiQ5E954. differential.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNJA1_BOVIN
AccessioniPrimary (citable) accession number: Q5E954
Secondary accession number(s): Q3SZU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: April 17, 2007
Last modified: November 30, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.