Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tubulin-folding cofactor B

Gene

TBCB

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Enzyme and pathway databases

ReactomeiR-BTA-389977. Post-chaperonin tubulin folding pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Tubulin-folding cofactor B
Alternative name(s):
Cytoskeleton-associated protein 1
Cytoskeleton-associated protein CKAPI
Tubulin-specific chaperone B
Gene namesi
Name:TBCB
Synonyms:CKAP1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 18

Subcellular locationi

  • Cytoplasm By similarity
  • Cytoplasmcytoskeleton By similarity

  • Note: Colocalizes with microtubules. In differentiated neurons, located in the cytoplasm. In differentiating neurons, accumulates at the growth cone (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 244244Tubulin-folding cofactor BPRO_0000083533Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei98 – 981PhosphotyrosineBy similarity
Modified residuei110 – 1101PhosphoserineBy similarity
Modified residuei219 – 2191N6-acetyllysineBy similarity

Post-translational modificationi

Ubiquitinated in the presence of GAN which targets it for degradation by the proteasome.By similarity
Phosphorylation by PAK1 is required for normal function.

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ5E951.
PeptideAtlasiQ5E951.
PRIDEiQ5E951.

Interactioni

Subunit structurei

Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state. Cofactors B and E can form a heterodimer which binds to alpha-tubulin and enhances their ability to dissociate tubulin heterodimers. Binds to GAN (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019973.

Structurei

3D structure databases

ProteinModelPortaliQ5E951.
SMRiQ5E951. Positions 11-93, 134-233.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini183 – 22543CAP-GlyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TBCB family.Curated
Contains 1 CAP-Gly domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3206. Eukaryota.
ENOG410YKGB. LUCA.
GeneTreeiENSGT00760000119173.
HOGENOMiHOG000209180.
HOVERGENiHBG003239.
InParanoidiQ5E951.
KOiK17262.
OMAiYNEEEMQ.
OrthoDBiEOG7C2R2R.
TreeFamiTF313444.

Family and domain databases

Gene3Di2.30.30.190. 1 hit.
InterProiIPR000938. CAP-Gly_domain.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF01302. CAP_GLY. 1 hit.
PF14560. Ubiquitin_2. 1 hit.
[Graphical view]
SMARTiSM01052. CAP_GLY. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF74924. SSF74924. 1 hit.
PROSITEiPS00845. CAP_GLY_1. 1 hit.
PS50245. CAP_GLY_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5E951-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVTGLSAPT VNVFISSSLN SFRSQKRYSR SLTVAEFKCK LQLVVGSPAS
60 70 80 90 100
CMELELYGPD DKFCCKLDQD DALLGSYPVD DGCRIHVIDH SGARLGEYED
110 120 130 140 150
ISKVEKYEIS QEAYEQRQDS IRSFLKRNKL GRFNEEERAQ QEAENSQRLI
160 170 180 190 200
EEEAQASTIP VGSRCEVRTP GQPPRRGTVM YVGLTDFKPG YWIGIRYDEP
210 220 230 240
LGKNDGSVNG KRYFECQAKY GAFVKPSVVT VGDFPEEDYG LDEM
Length:244
Mass (Da):27,518
Last modified:March 15, 2005 - v1
Checksum:iD135E9DA88998927
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021069 mRNA. Translation: AAX09086.1.
BC102571 mRNA. Translation: AAI02572.1.
RefSeqiNP_001014939.1. NM_001014939.1.
UniGeneiBt.55460.

Genome annotation databases

EnsembliENSBTAT00000019973; ENSBTAP00000019973; ENSBTAG00000015004.
GeneIDi530447.
KEGGibta:530447.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021069 mRNA. Translation: AAX09086.1.
BC102571 mRNA. Translation: AAI02572.1.
RefSeqiNP_001014939.1. NM_001014939.1.
UniGeneiBt.55460.

3D structure databases

ProteinModelPortaliQ5E951.
SMRiQ5E951. Positions 11-93, 134-233.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019973.

Proteomic databases

PaxDbiQ5E951.
PeptideAtlasiQ5E951.
PRIDEiQ5E951.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000019973; ENSBTAP00000019973; ENSBTAG00000015004.
GeneIDi530447.
KEGGibta:530447.

Organism-specific databases

CTDi1155.

Phylogenomic databases

eggNOGiKOG3206. Eukaryota.
ENOG410YKGB. LUCA.
GeneTreeiENSGT00760000119173.
HOGENOMiHOG000209180.
HOVERGENiHBG003239.
InParanoidiQ5E951.
KOiK17262.
OMAiYNEEEMQ.
OrthoDBiEOG7C2R2R.
TreeFamiTF313444.

Enzyme and pathway databases

ReactomeiR-BTA-389977. Post-chaperonin tubulin folding pathway.

Family and domain databases

Gene3Di2.30.30.190. 1 hit.
InterProiIPR000938. CAP-Gly_domain.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF01302. CAP_GLY. 1 hit.
PF14560. Ubiquitin_2. 1 hit.
[Graphical view]
SMARTiSM01052. CAP_GLY. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF74924. SSF74924. 1 hit.
PROSITEiPS00845. CAP_GLY_1. 1 hit.
PS50245. CAP_GLY_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.

Entry informationi

Entry nameiTBCB_BOVIN
AccessioniPrimary (citable) accession number: Q5E951
Secondary accession number(s): Q3ZCC1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: March 15, 2005
Last modified: July 6, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.