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Protein

Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase

Gene

gppA

Organism
Vibrio fischeri (strain ATCC 700601 / ES114)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Conversion of pppGpp to ppGpp. Guanosine pentaphosphate (pppGpp) is a cytoplasmic signaling molecule which together with ppGpp controls the "stringent response", an adaptive process that allows bacteria to respond to amino acid starvation, resulting in the coordinated regulation of numerous cellular activities.UniRule annotation

Catalytic activityi

Guanosine 5'-triphosphate,3'-diphosphate + H2O = guanosine 3',5'-bis(diphosphate) + phosphate.UniRule annotation

Pathway: ppGpp biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes ppGpp from GTP.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA)
  2. no protein annotated in this organism
This subpathway is part of the pathway ppGpp biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ppGpp from GTP, the pathway ppGpp biosynthesis and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciAFIS312309:GIWP-55-MONOMER.
UniPathwayiUPA00908; UER00884.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanosine-5'-triphosphate,3'-diphosphate pyrophosphataseUniRule annotation (EC:3.6.1.40UniRule annotation)
Alternative name(s):
Guanosine pentaphosphate phosphohydrolaseUniRule annotation
pppGpp-5'-phosphohydrolaseUniRule annotation
Gene namesi
Name:gppAUniRule annotation
Ordered Locus Names:VF_0054
OrganismiVibrio fischeri (strain ATCC 700601 / ES114)
Taxonomic identifieri312309 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
ProteomesiUP000000537 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 497497Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatasePRO_0000194293Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi312309.VF_0054.

Structurei

3D structure databases

ProteinModelPortaliQ5E8U7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GppA/Ppx family. GppA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0248.
HOGENOMiHOG000258672.
KOiK01524.
OMAiFKRAPQH.
OrthoDBiEOG6M6JN6.

Family and domain databases

HAMAPiMF_01550. GppA.
InterProiIPR023709. Guo-5TP_3DP_PyrP.
IPR003695. Ppx_GppA.
IPR030673. PyroPPase_GppA_Ppx.
[Graphical view]
PfamiPF02541. Ppx-GppA. 1 hit.
[Graphical view]
PIRSFiPIRSF001267. Pyrophosphatase_GppA_Ppx. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5E8U7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSSTMSPMY AAIDLGSNSF HMLVVRHING SVQTMAKIKR KVRLAAGLNE
60 70 80 90 100
NNTLSHEAMQ RGWDCLSLFA ERLQDIPVEN IRIVGTATLR VASNVDIFLE
110 120 130 140 150
KANQILGHNI NVIEGEEEAR MIYQGVAHTS GGNGRRLVVD IGGASTELII
160 170 180 190 200
GEGFEAQALT SLKMGCVTWL EGYFKDRALT QKNFNAAIAG AKETLAPILQ
210 220 230 240 250
QYTDLGWQTC VGASGTVQAL QEIMLAQGMD EVITLAKLKR LQKQAMQYEH
260 270 280 290 300
LEELDIDGLT LERALVFPSG LSILIAIFEL LNIDSMTLAG GALREGLCYG
310 320 330 340 350
MIDELQHDEV CQRTIKSTQQ RYQLDVDYAQ QVTDLSIQLV QQCGNDWLIE
360 370 380 390 400
PQALPLLTAA TQLHEIGMCI DYKKGGEHSA YLINALDLPG FTRAQKHLLG
410 420 430 440 450
ELLRRYREYF SAMPTQHAVS DISAQRMLRI LRLAIILTHR RDVNLAPTVT
460 470 480 490
LSEKNDVLSL SIDGAWLAAN PLTRSELEIE ADKQTNIGWE LVIDARD
Length:497
Mass (Da):55,115
Last modified:March 15, 2005 - v1
Checksum:i07F0B3258F73AB1B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000020 Genomic DNA. Translation: AAW84549.1.
RefSeqiWP_011260931.1. NC_006840.2.
YP_203437.1. NC_006840.2.

Genome annotation databases

EnsemblBacteriaiAAW84549; AAW84549; VF_0054.
GeneIDi3277951.
KEGGivfi:VF_0054.
PATRICi20110595. VBIVibFis127983_0055.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000020 Genomic DNA. Translation: AAW84549.1.
RefSeqiWP_011260931.1. NC_006840.2.
YP_203437.1. NC_006840.2.

3D structure databases

ProteinModelPortaliQ5E8U7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi312309.VF_0054.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW84549; AAW84549; VF_0054.
GeneIDi3277951.
KEGGivfi:VF_0054.
PATRICi20110595. VBIVibFis127983_0055.

Phylogenomic databases

eggNOGiCOG0248.
HOGENOMiHOG000258672.
KOiK01524.
OMAiFKRAPQH.
OrthoDBiEOG6M6JN6.

Enzyme and pathway databases

UniPathwayiUPA00908; UER00884.
BioCyciAFIS312309:GIWP-55-MONOMER.

Family and domain databases

HAMAPiMF_01550. GppA.
InterProiIPR023709. Guo-5TP_3DP_PyrP.
IPR003695. Ppx_GppA.
IPR030673. PyroPPase_GppA_Ppx.
[Graphical view]
PfamiPF02541. Ppx-GppA. 1 hit.
[Graphical view]
PIRSFiPIRSF001267. Pyrophosphatase_GppA_Ppx. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700601 / ES114.

Entry informationi

Entry nameiGPPA_VIBF1
AccessioniPrimary (citable) accession number: Q5E8U7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 15, 2005
Last modified: April 29, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.