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Q5E858

- LEUC_VIBF1

UniProt

Q5E858 - LEUC_VIBF1

Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Vibrio fischeri (strain ATCC 700601 / ES114)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (15 Mar 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

    Catalytic activityi

    (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi349 – 3491Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi409 – 4091Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi412 – 4121Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 3-isopropylmalate dehydratase activity Source: UniProtKB-HAMAP
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. leucine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciAFIS312309:GIWP-289-MONOMER.
    UniPathwayiUPA00048; UER00071.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
    Alternative name(s):
    Alpha-IPM isomeraseUniRule annotation
    Short name:
    IPMIUniRule annotation
    Isopropylmalate isomeraseUniRule annotation
    Gene namesi
    Name:leuCUniRule annotation
    Ordered Locus Names:VF_0293
    OrganismiVibrio fischeri (strain ATCC 700601 / ES114)
    Taxonomic identifieri312309 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
    ProteomesiUP000000537: Chromosome I

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4714713-isopropylmalate dehydratase large subunitPRO_0000076837Add
    BLAST

    Interactioni

    Subunit structurei

    Heterodimer of LeuC and LeuD.UniRule annotation

    Protein-protein interaction databases

    STRINGi312309.VF_0293.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5E858.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0065.
    HOGENOMiHOG000226972.
    KOiK01703.
    OMAiQARAKTM.
    OrthoDBiEOG600DP5.

    Family and domain databases

    Gene3Di3.30.499.10. 2 hits.
    3.40.1060.10. 1 hit.
    HAMAPiMF_01026. LeuC_type1.
    InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
    IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
    IPR015937. Acoase/IPM_deHydtase.
    IPR001030. Acoase/IPM_deHydtase_lsu_aba.
    IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
    IPR018136. Aconitase_4Fe-4S_BS.
    [Graphical view]
    PANTHERiPTHR11670. PTHR11670. 1 hit.
    PfamiPF00330. Aconitase. 1 hit.
    [Graphical view]
    PRINTSiPR00415. ACONITASE.
    SUPFAMiSSF53732. SSF53732. 1 hit.
    TIGRFAMsiTIGR00170. leuC. 1 hit.
    PROSITEiPS00450. ACONITASE_1. 1 hit.
    PS01244. ACONITASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5E858-1 [UniParc]FASTAAdd to Basket

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    MSNAKTLYEK IYDAHVAVAA EGENPILYID RHLVHEVTSP QAFDGLREKG    50
    RKVRQVGKTF ATMDHNVSTQ TKDINASGEM ARIQMETLSK NCEEFGVTLY 100
    DLNHKYQGIV HVMGPELGIT LPGMTIVCGD SHTATHGAFG SLAFGIGTSE 150
    VEHVLATQTL KQARAKTMKI DVKGKVAEGI TAKDIVLAII GKTTAAGGTG 200
    YVVEFCGEAI TDLTMEGRMT VCNMAIELGA KAGLIAPDQT TFDYIAGRKF 250
    SPQDADLDAA IEYWSSLKTD DDAEFDAVVT LDASEIKPQV TWGTNPGQVI 300
    AVDAPIPAPE SFADPVEKAS AEKALAYMGL EAGKSLSDYN VDKVFVGSCT 350
    NSRIEDMRAA AAIAKGRKVA SHVQALIVPG SEQVKAQAEK EGLDVIFKEA 400
    GFEWRLPGCS MCLAMNNDRL GPHERCASTS NRNFEGRQGR DGRTHLVSPA 450
    MAAAAAIAGH FVDIRTITEQ A 471
    Length:471
    Mass (Da):50,470
    Last modified:March 15, 2005 - v1
    Checksum:iCA514DE363251123
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000020 Genomic DNA. Translation: AAW84788.1.
    RefSeqiYP_203676.1. NC_006840.2.

    Genome annotation databases

    EnsemblBacteriaiAAW84788; AAW84788; VF_0293.
    GeneIDi3277251.
    KEGGivfi:VF_0293.
    PATRICi20111060. VBIVibFis127983_0287.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000020 Genomic DNA. Translation: AAW84788.1 .
    RefSeqi YP_203676.1. NC_006840.2.

    3D structure databases

    ProteinModelPortali Q5E858.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 312309.VF_0293.

    Protocols and materials databases

    DNASUi 3277251.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAW84788 ; AAW84788 ; VF_0293 .
    GeneIDi 3277251.
    KEGGi vfi:VF_0293.
    PATRICi 20111060. VBIVibFis127983_0287.

    Phylogenomic databases

    eggNOGi COG0065.
    HOGENOMi HOG000226972.
    KOi K01703.
    OMAi QARAKTM.
    OrthoDBi EOG600DP5.

    Enzyme and pathway databases

    UniPathwayi UPA00048 ; UER00071 .
    BioCyci AFIS312309:GIWP-289-MONOMER.

    Family and domain databases

    Gene3Di 3.30.499.10. 2 hits.
    3.40.1060.10. 1 hit.
    HAMAPi MF_01026. LeuC_type1.
    InterProi IPR004430. 3-IsopropMal_deHydase_lsu.
    IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
    IPR015937. Acoase/IPM_deHydtase.
    IPR001030. Acoase/IPM_deHydtase_lsu_aba.
    IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
    IPR018136. Aconitase_4Fe-4S_BS.
    [Graphical view ]
    PANTHERi PTHR11670. PTHR11670. 1 hit.
    Pfami PF00330. Aconitase. 1 hit.
    [Graphical view ]
    PRINTSi PR00415. ACONITASE.
    SUPFAMi SSF53732. SSF53732. 1 hit.
    TIGRFAMsi TIGR00170. leuC. 1 hit.
    PROSITEi PS00450. ACONITASE_1. 1 hit.
    PS01244. ACONITASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700601 / ES114.

    Entry informationi

    Entry nameiLEUC_VIBF1
    AccessioniPrimary (citable) accession number: Q5E858
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: March 15, 2005
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3