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Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Vibrio fischeri (strain ATCC 700601 / ES114)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: L-leucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 2-isopropylmalate synthase (leuA)
  2. 3-isopropylmalate dehydratase small subunit (leuD), 3-isopropylmalate dehydratase large subunit (leuC)
  3. 3-isopropylmalate dehydrogenase (leuB)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi349Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi409Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi412Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciVFIS312309:G1G40-289-MONOMER
UniPathwayiUPA00048; UER00071

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomeraseUniRule annotation
Short name:
IPMIUniRule annotation
Isopropylmalate isomeraseUniRule annotation
Gene namesi
Name:leuCUniRule annotation
Ordered Locus Names:VF_0293
OrganismiVibrio fischeri (strain ATCC 700601 / ES114)
Taxonomic identifieri312309 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
Proteomesi
  • UP000000537 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000768371 – 4713-isopropylmalate dehydratase large subunitAdd BLAST471

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotation

Protein-protein interaction databases

STRINGi312309.VF_0293

Structurei

3D structure databases

ProteinModelPortaliQ5E858
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQI Bacteria
COG0065 LUCA
HOGENOMiHOG000226972
KOiK01703
OMAiFDHQVPA
OrthoDBiPOG091H02GD

Family and domain databases

CDDicd01583 IPMI, 1 hit
Gene3Di3.30.499.10, 1 hit
HAMAPiMF_01026 LeuC_type1, 1 hit
InterProiView protein in InterPro
IPR004430 3-IsopropMal_deHydase_lsu
IPR015931 Acnase/IPM_dHydase_lsu_aba_1/3
IPR001030 Acoase/IPM_deHydtase_lsu_aba
IPR018136 Aconitase_4Fe-4S_BS
IPR036008 Aconitase_4Fe-4S_dom
IPR033941 IPMI_cat
PfamiView protein in Pfam
PF00330 Aconitase, 1 hit
PRINTSiPR00415 ACONITASE
SUPFAMiSSF53732 SSF53732, 1 hit
TIGRFAMsiTIGR00170 leuC, 1 hit
PROSITEiView protein in PROSITE
PS00450 ACONITASE_1, 1 hit
PS01244 ACONITASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q5E858-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNAKTLYEK IYDAHVAVAA EGENPILYID RHLVHEVTSP QAFDGLREKG
60 70 80 90 100
RKVRQVGKTF ATMDHNVSTQ TKDINASGEM ARIQMETLSK NCEEFGVTLY
110 120 130 140 150
DLNHKYQGIV HVMGPELGIT LPGMTIVCGD SHTATHGAFG SLAFGIGTSE
160 170 180 190 200
VEHVLATQTL KQARAKTMKI DVKGKVAEGI TAKDIVLAII GKTTAAGGTG
210 220 230 240 250
YVVEFCGEAI TDLTMEGRMT VCNMAIELGA KAGLIAPDQT TFDYIAGRKF
260 270 280 290 300
SPQDADLDAA IEYWSSLKTD DDAEFDAVVT LDASEIKPQV TWGTNPGQVI
310 320 330 340 350
AVDAPIPAPE SFADPVEKAS AEKALAYMGL EAGKSLSDYN VDKVFVGSCT
360 370 380 390 400
NSRIEDMRAA AAIAKGRKVA SHVQALIVPG SEQVKAQAEK EGLDVIFKEA
410 420 430 440 450
GFEWRLPGCS MCLAMNNDRL GPHERCASTS NRNFEGRQGR DGRTHLVSPA
460 470
MAAAAAIAGH FVDIRTITEQ A
Length:471
Mass (Da):50,470
Last modified:March 15, 2005 - v1
Checksum:iCA514DE363251123
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000020 Genomic DNA Translation: AAW84788.1
RefSeqiWP_011261103.1, NC_006840.2
YP_203676.1, NC_006840.2

Genome annotation databases

EnsemblBacteriaiAAW84788; AAW84788; VF_0293
GeneIDi3277251
KEGGivfi:VF_0293
PATRICifig|312309.11.peg.287

Similar proteinsi

Entry informationi

Entry nameiLEUC_VIBF1
AccessioniPrimary (citable) accession number: Q5E858
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: March 15, 2005
Last modified: March 28, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health