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Q5E858

- LEUC_VIBF1

UniProt

Q5E858 - LEUC_VIBF1

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Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Vibrio fischeri (strain ATCC 700601 / ES114)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi349 – 3491Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi409 – 4091Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi412 – 4121Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 3-isopropylmalate dehydratase activity Source: UniProtKB-HAMAP
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. leucine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciAFIS312309:GIWP-289-MONOMER.
UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomeraseUniRule annotation
Short name:
IPMIUniRule annotation
Isopropylmalate isomeraseUniRule annotation
Gene namesi
Name:leuCUniRule annotation
Ordered Locus Names:VF_0293
OrganismiVibrio fischeri (strain ATCC 700601 / ES114)
Taxonomic identifieri312309 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
ProteomesiUP000000537: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4714713-isopropylmalate dehydratase large subunitPRO_0000076837Add
BLAST

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotation

Protein-protein interaction databases

STRINGi312309.VF_0293.

Structurei

3D structure databases

ProteinModelPortaliQ5E858.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0065.
HOGENOMiHOG000226972.
KOiK01703.
OMAiQARAKTM.
OrthoDBiEOG600DP5.

Family and domain databases

Gene3Di3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01026. LeuC_type1.
InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR00170. leuC. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5E858-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNAKTLYEK IYDAHVAVAA EGENPILYID RHLVHEVTSP QAFDGLREKG
60 70 80 90 100
RKVRQVGKTF ATMDHNVSTQ TKDINASGEM ARIQMETLSK NCEEFGVTLY
110 120 130 140 150
DLNHKYQGIV HVMGPELGIT LPGMTIVCGD SHTATHGAFG SLAFGIGTSE
160 170 180 190 200
VEHVLATQTL KQARAKTMKI DVKGKVAEGI TAKDIVLAII GKTTAAGGTG
210 220 230 240 250
YVVEFCGEAI TDLTMEGRMT VCNMAIELGA KAGLIAPDQT TFDYIAGRKF
260 270 280 290 300
SPQDADLDAA IEYWSSLKTD DDAEFDAVVT LDASEIKPQV TWGTNPGQVI
310 320 330 340 350
AVDAPIPAPE SFADPVEKAS AEKALAYMGL EAGKSLSDYN VDKVFVGSCT
360 370 380 390 400
NSRIEDMRAA AAIAKGRKVA SHVQALIVPG SEQVKAQAEK EGLDVIFKEA
410 420 430 440 450
GFEWRLPGCS MCLAMNNDRL GPHERCASTS NRNFEGRQGR DGRTHLVSPA
460 470
MAAAAAIAGH FVDIRTITEQ A
Length:471
Mass (Da):50,470
Last modified:March 15, 2005 - v1
Checksum:iCA514DE363251123
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000020 Genomic DNA. Translation: AAW84788.1.
RefSeqiWP_011261103.1. NC_006840.2.
YP_203676.1. NC_006840.2.

Genome annotation databases

EnsemblBacteriaiAAW84788; AAW84788; VF_0293.
GeneIDi3277251.
KEGGivfi:VF_0293.
PATRICi20111060. VBIVibFis127983_0287.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000020 Genomic DNA. Translation: AAW84788.1 .
RefSeqi WP_011261103.1. NC_006840.2.
YP_203676.1. NC_006840.2.

3D structure databases

ProteinModelPortali Q5E858.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 312309.VF_0293.

Protocols and materials databases

DNASUi 3277251.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAW84788 ; AAW84788 ; VF_0293 .
GeneIDi 3277251.
KEGGi vfi:VF_0293.
PATRICi 20111060. VBIVibFis127983_0287.

Phylogenomic databases

eggNOGi COG0065.
HOGENOMi HOG000226972.
KOi K01703.
OMAi QARAKTM.
OrthoDBi EOG600DP5.

Enzyme and pathway databases

UniPathwayi UPA00048 ; UER00071 .
BioCyci AFIS312309:GIWP-289-MONOMER.

Family and domain databases

Gene3Di 3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPi MF_01026. LeuC_type1.
InterProi IPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view ]
PANTHERi PTHR11670. PTHR11670. 1 hit.
Pfami PF00330. Aconitase. 1 hit.
[Graphical view ]
PRINTSi PR00415. ACONITASE.
SUPFAMi SSF53732. SSF53732. 1 hit.
TIGRFAMsi TIGR00170. leuC. 1 hit.
PROSITEi PS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700601 / ES114.

Entry informationi

Entry nameiLEUC_VIBF1
AccessioniPrimary (citable) accession number: Q5E858
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: March 15, 2005
Last modified: October 29, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3