Reviewed, UniProtKB/Swiss-Prot Q5E7R0 (E4PD_VIBF1)

Names and origin

Protein names

Recommended name:
    D-erythrose-4-phosphate dehydrogenase
      Short name=E4PDH
    EC=1.2.1.72

Gene names

Name:	epd
Ordered Locus Names:	VF_0441

Organism

Vibrio fischeri (strain ATCC 700601 / ES114) [Complete proteome] [HAMAP]

Taxonomic identifier

312309 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD-dependent conversion of D-erythrose 4-phosphate to 4-phosphoerythronate By similarity.
Catalytic activity	D-erythrose 4-phosphate + NAD(+) + H(2)O = 4-phosphoerythronate + NADH.
Pathway	Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. Epd subfamily.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridoxal phosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP pyridoxine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro erythrose-4-phosphate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	D-erythrose-4-phosphate dehydrogenase		PRO_0000293174
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	158 – 160	3	Substrate binding Potential
	Region	217 – 218	2	Substrate binding Potential
Sites
	Active site	159	1	Nucleophile By similarity
	Binding site	204	1	Substrate Potential
	Binding site	240	1	Substrate Potential
	Binding site	322	1	NAD By similarity
	Site	186	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5E7R0-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 1D03481B431CE114	FASTA	339	37,603
	10 20 30 40 50 60 MLRVAINGFG RIGRSVLRAL YESDKRDKIE VVAVNELSQP EAMAHLFQYD STHGRFQHKV 70 80 90 100 110 120 THDQEYPYSD VPDSSQDKVR ILHQADLSLL PWQSLEVDLV LDCTGVYGSK SDGEKHITAG 130 140 150 160 170 180 AKKVLFSHPG GSDLDNTIIY GVNHDTLLPE HRIVSNGSCT TNCIIPVIKA IDDAFGIDSG 190 200 210 220 230 240 TITTIHSSMN DQQVIDAYHS DLRRTRAASQ SIIPVDTKLH KGIERIFPKF SNKFEAISVR 250 260 270 280 290 300 VPTVNVTAMD LSVTINTNVK VNDINQTIVN ASRCTLHNIV DYTEAPLVSI DFNHDPHSAI 310 320 330 VDGSQTRVSN GHLVKMLVWC DNEWGFANRM LDTALAMSK

References

[1]

"Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congeners." Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R., Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E., Stevens A., Visick K., Whistler C., Greenberg E.P. Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005) [PubMed: 15703294] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000020 Genomic DNA. Translation: AAW84936.1.
RefSeq	YP_203824.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3278161.
GenomeReviews	Gene locus VF_0441 in contig CP000020_GR.
KEGG	vfi:VF0441.
NMPDR	fig|312309.3.peg.441.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5E7R0.
Enzyme and pathway databases
BioCyc	VFIS312309:VF0441-MON.
Family and domain databases
HAMAP	MF_01640. [Tree]
InterPro	IPR006422. E4P_DHase_bac. IPR000173. GlycerAld_3-P_DHase. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01532. E4PD_g-proteo. 1 hit.
PROSITE	PS00071. GAPDH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E4PD_VIBF1

Accession

Primary (citable) accession number: Q5E7R0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	March 15, 2005
Last modified:	November 25, 2008
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)