Q5E7P1 (TYSY_VIBF1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	VF_0460

Organism

Vibrio fischeri (strain ATCC 700601 / ES114)

Taxonomic identifier

312309 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity. HAMAP MF_00008
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.
Sequence caution	The sequence AAW84955.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	Thymidylate synthase HAMAP MF_00008		PRO_0000141040
Sites
	Active site	160	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5E7P1 [UniParc]. Last modified August 2, 2005. Version 2. Checksum: F08B6D437257946F	FASTA	283	32,464
	10 20 30 40 50 60 MKQYLDLCQR IVDEGTWIEN ERTGKRCLTV INADLTYDVE NGVFPLVTTR KSFWKAAVAE 70 80 90 100 110 120 MIGYIRGYDS AEDFRKIGTK TWDANANLND AWLNNPHRKG DDDMGRVYGV QGRSWAKPNG 130 140 150 160 170 180 EFVDQLKKII DDLSQGIDDR GEILNFYNPG EFDMGCLRPC MYSHHFSLLG DTLYLNSTQR 190 200 210 220 230 240 SCDVPLGLNF NMVQVYFLLA IVAQITGHKA GKAYHKIVNA HIYEDQLELM QSVQLKREPL 250 260 270 280 QAPIFKINPE IKSLEDLETW VTMDDFEVIG YESHEAIKYP FSV

References

[1]

"Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congeners." Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R., Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E., Stevens A., Visick K., Whistler C., Greenberg E.P. Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005) [PubMed: 15703294] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700601 / ES114.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000020 Genomic DNA. Translation: AAW84955.1. Different initiation.
RefSeq	YP_203843.1. NC_006840.2.
3D structure databases
ProteinModelPortal	Q5E7P1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5E7P1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3277469.
GenomeReviews	Gene locus VF_0460 in contig CP000020_GR.
KEGG	vfi:VF_0460.
NMPDR	fig|312309.3.peg.459.
PATRIC	20111426. VBIVibFis127983_0450.
Phylogenomic databases
eggNOG	COG0207.
HOGENOM	HBG588098.
OMA	ANGSREF.
PhylomeDB	Q5E7P1.
ProtClustDB	PRK01827.
Enzyme and pathway databases
BioCyc	VFIS312309:VF0460-MONOMER.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
KO	K00560.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. Thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_VIBF1

Accession

Primary (citable) accession number: Q5E7P1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2005
Last sequence update:	August 2, 2005
Last modified:	January 25, 2012
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program