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Q5E7L5 (IF2_VIBF1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Translation initiation factor IF-2
Gene names
Name:infB
Ordered Locus Names:VF_0486
OrganismVibrio fischeri (strain ATCC 700601 / ES114) [Reference proteome] [HAMAP]
Taxonomic identifier312309 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio

Protein attributes

Sequence length893 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00100.

Sequence similarities

Belongs to the IF-2 family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   Molecular functionInitiation factor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

translation initiation factor activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 893893Translation initiation factor IF-2 HAMAP-Rule MF_00100
PRO_0000228258

Regions

Nucleotide binding401 – 4088GTP By similarity
Nucleotide binding447 – 4515GTP By similarity
Nucleotide binding501 – 5044GTP By similarity
Region395 – 543149G-domain HAMAP-Rule MF_00100

Sequences

Sequence LengthMass (Da)Tools
Q5E7L5 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 7FE41029382211C6

FASTA89397,555
        10         20         30         40         50         60 
MTKLTVKALS EEIGTPVDRL LQQFSDAGIN KKDGDSVTES EKQSLLVHLK KEHGSVDDSA 

        70         80         90        100        110        120 
SPTRLTLQRK TRSTLSVAGS GGKSKDVQVE VRKKRTYVKA SALEEEKKAE QLKAEAEEQA 

       130        140        150        160        170        180 
KRDAEEAAVR ELEQKAQREA EEQAKREAEA EAKAKREAEE KAKRAEADKA KKEMTKKNEQ 

       190        200        210        220        230        240 
AKKEAEELKA RQELEATRKA EAEAAKLVEE ARKLAEENEA RWKEEEQKKS AAEKDADYHV 

       250        260        270        280        290        300 
TTSSHAREAE DAADRKEEQQ PRRRKKKAKP AEAAAPRGGR NQRGGRNKKA QVNKPTSMQH 

       310        320        330        340        350        360 
GFDKSATVAK QDVAIGETIV VSELASKMSV KATEVIKVMM KMGAMATINQ VIDQETAQLV 

       370        380        390        400        410        420 
AEEMGHKVIL RKENELEEAV LSDRDNSAEA EGRAPVVTIM GHVDHGKTST LDYIRRAHVA 

       430        440        450        460        470        480 
DAEAGGITQH IGAYHVETDN GMITFLDTPG HAAFTAMRAR GAQATDIVVL VVAADDGVMP 

       490        500        510        520        530        540 
QTIEAIQHAK AAGVPLIVAV NKIDKEDANP DNVKNELAQY DVIPEEWGGE NMFVHISAKQ 

       550        560        570        580        590        600 
GTNIDGLLEA ILLQSEVLEL TAVREGMASG VVVESRLDKG RGPVATVLVQ SGTLNKGDIV 

       610        620        630        640        650        660 
LCGQEYGRVR AMRDENGKEI ESAGPSIPVE ILGLSGVPAS GDEATVVRDE RKAREVANYR 

       670        680        690        700        710        720 
QGKFRDVKLA RQQKAKLENM FSNMTAGEVA ELNVVLKADV QGSVEAIADS LRKLSTDEVK 

       730        740        750        760        770        780 
VNIVGSGVGG ITETDAVLAA ASNAIILGFN VRADATARRT IENENLDLRY YSIIYQLIDE 

       790        800        810        820        830        840 
VKAAMGGMLA PEFKQEIIGL AQVRDVFKSP KLGAIAGCMV TEGTIKRSNP IRVLRDNVVI 

       850        860        870        880        890 
YEGELESLRR FKDDVAEVKN GYECGIGVKN YNDVRVGDQI EVFEIVEIKR TLD 

« Hide

References

[1]"Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congeners."
Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R., Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E., Stevens A., Visick K., Whistler C., Greenberg E.P.
Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700601 / ES114.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000020 Genomic DNA. Translation: AAW84981.1.
RefSeqYP_203869.1. NC_006840.2.

3D structure databases

ProteinModelPortalQ5E7L5.
SMRQ5E7L5. Positions 2-50.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING312309.VF_0486.

Proteomic databases

PRIDEQ5E7L5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAW84981; AAW84981; VF_0486.
GeneID3278169.
KEGGvfi:VF_0486.
PATRIC20111482. VBIVibFis127983_0476.

Phylogenomic databases

eggNOGCOG0532.
HOGENOMHOG000076907.
KOK02519.
OMADNRTGGY.
OrthoDBEOG67HJSV.

Enzyme and pathway databases

BioCycAFIS312309:GIWP-503-MONOMER.

Family and domain databases

Gene3D3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00100_B. IF_2_B.
InterProIPR009061. DNA-bd_dom_put.
IPR000795. EF_GTP-bd_dom.
IPR013575. IF2_assoc_dom_bac.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 2 hits.
PF11987. IF-2. 1 hit.
PF08364. IF2_assoc. 1 hit.
PF04760. IF2_N. 2 hits.
[Graphical view]
SUPFAMSSF46955. SSF46955. 1 hit.
SSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS01176. IF2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIF2_VIBF1
AccessionPrimary (citable) accession number: Q5E7L5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: March 15, 2005
Last modified: May 14, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Translation initiation factors

List of translation initiation factor entries