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Q5E7L1

- PNP_VIBF1

UniProt

Q5E7L1 - PNP_VIBF1

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Protein

Polyribonucleotide nucleotidyltransferase

Gene
pnp, VF_0490
Organism
Vibrio fischeri (strain ATCC 700601 / ES114)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi487 – 4871Magnesium By similarity
Metal bindingi493 – 4931Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciAFIS312309:GIWP-507-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:VF_0490
OrganismiVibrio fischeri (strain ATCC 700601 / ES114)
Taxonomic identifieri312309 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
ProteomesiUP000000537: Chromosome I

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 709709Polyribonucleotide nucleotidyltransferaseUniRule annotationPRO_0000329929Add
BLAST

Proteomic databases

PRIDEiQ5E7L1.

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation By similarity.

Protein-protein interaction databases

STRINGi312309.VF_0490.

Structurei

3D structure databases

ProteinModelPortaliQ5E7L1.
SMRiQ5E7L1. Positions 618-693.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini554 – 61360KHAdd
BLAST
Domaini623 – 69169S1 motifAdd
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5E7L1-1 [UniParc]FASTAAdd to Basket

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MFANPVVKSF QYGNHTVTLE TGVIARQATA AVMASMDDTS VFVSVVAKKE    50
AVPGQDFFPL TVNYQERTYA AGKIPGGFFK REGRPSEAET LTARLIDRPI 100
RPLFPDAFKN EVQVIATVVS INPEVNPDMI TMIGTSAALA IAGIPFNGPI 150
GAARVGHING ELVLNPSNTE LENSKLDLVV SGTEGAVLMV ESEADNLTEE 200
EMLSAVVFGH EQQQVVINAI NEFAAEVATP AWDWVAPEEN TVLNARIAEL 250
AEAKLVEAYQ ITEKMTRYDR IHEIAAEVNE VLVSENEDVN LKEVHTIFHD 300
LEKTVVRRSI IAGNPRIDGR EKDMVRALDV RTGVLPRTHG SSLFTRGETQ 350
ALVTATLGTQ RDAQIIDSLM GEKKDHFLLH YNFPPYCVGE TGFVGSPKRR 400
EIGHGKLAKR GIQAVMPSIE EFPYTVRVVS EITESNGSSS MASVCGTSLA 450
LMDAGVPIKA SVAGIAMGLV KEGDDFVVLS DILGDEDHLG DMDFKVAGTN 500
EGITALQMDI KIEGITKEIM QIALNQAQGA RKHILKVMDE AISGAREEIS 550
EFAPRIHTMK ISSDKIKDVI GKGGAVIRAL CEETGTTIEI EDDGTIKIAA 600
TEGAAAKEAI RRIEEITAEV EVGKIYTGKV MRIVDFGAFV TVLGPKEGLV 650
HISQIAEERI EKVADHLQVG QEVKTKVLEI DRQGRIRLSI KEANAELNPA 700
PAAEAKDAE 709
Length:709
Mass (Da):76,835
Last modified:March 15, 2005 - v1
Checksum:i7A6EAADDCDB1BE33
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000020 Genomic DNA. Translation: AAW84985.1.
RefSeqiYP_203873.1. NC_006840.2.

Genome annotation databases

EnsemblBacteriaiAAW84985; AAW84985; VF_0490.
GeneIDi3278250.
KEGGivfi:VF_0490.
PATRICi20111490. VBIVibFis127983_0480.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000020 Genomic DNA. Translation: AAW84985.1 .
RefSeqi YP_203873.1. NC_006840.2.

3D structure databases

ProteinModelPortali Q5E7L1.
SMRi Q5E7L1. Positions 618-693.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 312309.VF_0490.

Proteomic databases

PRIDEi Q5E7L1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAW84985 ; AAW84985 ; VF_0490 .
GeneIDi 3278250.
KEGGi vfi:VF_0490.
PATRICi 20111490. VBIVibFis127983_0480.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci AFIS312309:GIWP-507-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700601 / ES114.

Entry informationi

Entry nameiPNP_VIBF1
AccessioniPrimary (citable) accession number: Q5E7L1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 15, 2005
Last modified: May 14, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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