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Q5E749 (PUR4_VIBF1) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylformylglycinamidine synthase
Short name=FGAM synthase
Short name=FGAMS
EC=6.3.5.3
Alternative name(s):
Formylglycinamide ribotide amidotransferase
Short name=FGARAT
Formylglycinamide ribotide synthetase
Gene names
Name:purL
Ordered Locus Names:VF_0652
OrganismVibrio fischeri (strain ATCC 700601 / ES114)
Taxonomic identifier312309 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length1303 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419

Subunit structure

Monomer.

Subcellular location

Cytoplasm HAMAP MF_00419.

Sequence similarities

In the N-terminal section; belongs to the FGAMS family.

Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   DomainGlutamine amidotransferase
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

glutamine metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoribosylformylglycinamidine synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13031303Phosphoribosylformylglycinamidine synthase HAMAP MF_00419
PRO_0000264601

Regions

Domain1050 – 1303254Glutamine amidotransferase type-1
Nucleotide binding308 – 31912ATP Potential

Sites

Active site11431Nucleophile By similarity
Active site12681 By similarity
Active site12701 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5E749 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: F07B7FE510D29C72

FASTA1,303142,181
        10         20         30         40         50         60 
MRILRGSPAL SEFRVNKLLE LCRELSLPVT GIYAEFAHFA DVTAELDASE VQKLEKLLTY 

        70         80         90        100        110        120 
GPTIEEHEPE GLLLLTTPRP GTISPWSSKS TDIAHNCGLD KIARLERGTA FYIERSEELS 

       130        140        150        160        170        180 
ELQLIELKAI LHDRMMEVVF TDFESASALF AVSEPAPYTE VDLLTGGRKA LEDANVTLGL 

       190        200        210        220        230        240 
ALAEDEIDYL LESFTEKLER NPTDIELMMF AQANSEHCRH KIFNADWTID GVKQEKSLFK 

       250        260        270        280        290        300 
MIKNTFEVTP DNVLSAYKDN AAVMTGSEVG RFFPDPETRQ YNYHQEKTHI LMKVETHNHP 

       310        320        330        340        350        360 
TAISPWPGAS TGSGGEIRDE GATGIGGKPK AGLVAFSVSN LKIPNFVQPW ETDFGKPSRI 

       370        380        390        400        410        420 
VTALDIMLEG PLGGAAFNNE FGRPNLLGYF RTYEEKVNSH AGEEVRGYHK PIMLAGGLGN 

       430        440        450        460        470        480 
IRDEHVQKKE IPVGASLIVL GGPAMNIGLG GGAASSMDSG SSSEDLDFAS VQRENPEMER 

       490        500        510        520        530        540 
RCQEVIDRCW QLGDANPIAF IHDVGAGGIS NALPELVDDG ERGGIFNLRD VPNDEPGMSP 

       550        560        570        580        590        600 
LEIWCNESQE RYVMAVADKD MATFDAICKR ERAPYAVVGK ATEERELKLE DSHFDNTPID 

       610        620        630        640        650        660 
MPMDILLGKT PKMHRDAKTL KANNPAIDRS GIEMNEAVDR VLRLPTVAEK TFLITIGDRS 

       670        680        690        700        710        720 
VTGLVARDQM VGPWQVPVAN CAVTAASYDS YHGEAMSLGE RTPVALLDFG ASARLAVGEA 

       730        740        750        760        770        780 
ITNIAATNIG DIKHIKLSAN WMSPAGHPGE DAGLYEAVKA VGEELCPALG LTIPVGKDSM 

       790        800        810        820        830        840 
SMKTKWEENG EQKEVTSPLS LVITAFARVE DVRKTITPQL RTPDNLEGLG DTSLVLIDLG 

       850        860        870        880        890        900 
NGKNRLGATA LAQVYKQLGD KPADVDNAAQ LKGFYEGVQA LVANDQVVAY HDKGDGGLFV 

       910        920        930        940        950        960 
TLAEMAFAGH CGVNANIEAL GEDTLAALFN EELGAVIQVR NDDLDAVLST LAANGLEACS 

       970        980        990       1000       1010       1020 
HVIGSVEASD ELVIKSGESV VIERNRTELR TIWAETTHKM QGLRDNPACA DQEHEAKKDN 

      1030       1040       1050       1060       1070       1080 
SDPGLNVKLS FDVNEDIAAP FINTGAKPKM AILREQGVNS HVEMAAAFDR AGFEATDIHM 

      1090       1100       1110       1120       1130       1140 
SDILTGQAVL EEYNGLVACG GFSYGDVLGA GEGWAKSVLF NDSTRDQFEN FFKREDTFSL 

      1150       1160       1170       1180       1190       1200 
GVCNGCQMLS NLRDLIPGAE YWPRFVRNES ERFEARFSLV EVQKSDSVFF NGMEGSRMPI 

      1210       1220       1230       1240       1250       1260 
AVSHGEGRVE VRDNDHLNAI ENSGTVALRY VDNHGNPTQQ YPNNPNGSPN AITGLTTTDG 

      1270       1280       1290       1300 
RVTIMMPHPE RVFRTVANSW SPEGWGENGA WMRMFQNARK NIG

« Hide

References

[1]"Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congeners."
Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R., Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E., Stevens A., Visick K., Whistler C., Greenberg E.P.
Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005) [PubMed: 15703294] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700601 / ES114.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000020 Genomic DNA. Translation: AAW85147.1.
RefSeqYP_204035.1. NC_006840.2.

3D structure databases

HSSPHSSP built from PDB template 1T3T based on UniProtKB P74881.
ProteinModelPortalQ5E749.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ5E749.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3277361.
GenomeReviewsGene locus VF_0652 in contig CP000020_GR.
KEGGvfi:VF_0652.
NMPDRfig|312309.3.peg.1153.
PATRIC20111848. VBIVibFis127983_0645.

Phylogenomic databases

eggNOGCOG0046.
HOGENOMHBG335309.
OMAVKAVGME.
PhylomeDBQ5E749.
ProtClustDBPRK05297.

Enzyme and pathway databases

BioCycVFIS312309:VF0652-MONOMER.

Family and domain databases

HAMAPMF_00419. PurL_1.
[Tree]
InterProIPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR017926. GATASE_1.
IPR010073. PRibForGlyAmidine_synth.
IPR022940. PRibForGlyAmidine_synth_bac.
IPR016188. PurM_N-like.
[Graphical view]
KOK01952.
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMSSF56042. AIR_synth_C. 2 hits.
SSF55326. PurM_N-like. 2 hits.
TIGRFAMsTIGR01735. FGAM_synt. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR4_VIBF1
AccessionPrimary (citable) accession number: Q5E749
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: March 15, 2005
Last modified: January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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