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Protein

Urease subunit alpha

Gene

ureC

Organism
Vibrio fischeri (strain ATCC 700601 / ES114)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation

Cofactori

Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit beta (ureB), Urease subunit gamma (ureA), Urease subunit alpha (ureC)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi134 – 1341Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi136 – 1361Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi217 – 2171Nickel 1; via carbamate groupUniRule annotation
Metal bindingi217 – 2171Nickel 2; via carbamate groupUniRule annotation
Binding sitei219 – 2191SubstrateUniRule annotation
Metal bindingi246 – 2461Nickel 2; via pros nitrogenUniRule annotation
Metal bindingi272 – 2721Nickel 2; via tele nitrogenUniRule annotation
Active sitei320 – 3201Proton donorUniRule annotation
Metal bindingi360 – 3601Nickel 1UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

BioCyciAFIS312309:GIWP-708-MONOMER.
UniPathwayiUPA00258; UER00370.

Protein family/group databases

MEROPSiM38.982.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alphaUniRule annotation
Gene namesi
Name:ureCUniRule annotation
Ordered Locus Names:VF_0673
OrganismiVibrio fischeri (strain ATCC 700601 / ES114)
Taxonomic identifieri312309 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
Proteomesi
  • UP000000537 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 567567Urease subunit alphaPRO_0000234192Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei217 – 2171N6-carboxylysineUniRule annotation

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Protein-protein interaction databases

STRINGi312309.VF_0673.

Structurei

3D structure databases

ProteinModelPortaliQ5E728.
SMRiQ5E728. Positions 2-567.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini129 – 567439UreaseUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the urease family.UniRule annotation
Contains 1 urease domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
HOGENOMiHOG000075064.
KOiK01428.
OMAiFDSHIHF.
OrthoDBiEOG6ND0GM.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5E728-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHISRTAYA NMFGPTTGDR LRLADTELFL EVEKDFTTYG EEVKFGGGKV
60 70 80 90 100
IRDGMGQSQV VNSECVDVVI TNALILDHWG IVKADIGIKD GRIFGIGKAG
110 120 130 140 150
NPDVQPNVDI VVGPATEVVA GEGKIITAGG VDTHIHFICP QQAEEGLTSG
160 170 180 190 200
VTTFIGGGTG PVAGTNATTV TPGIWNMHRM LEAVDDLPIN VGLFGKGCVS
210 220 230 240 250
KPEALREQIE AGAMGLKIHE DWGATPAAIH NCLNVADEMD IQIAIHSDTL
260 270 280 290 300
NEGGFYEETV KAIGDRVIHV FHTEGAGGGH APDVIKSVGE PNILPASTNP
310 320 330 340 350
TMPYTINTVD EHLDMLMVCH HLDPSIPEDV AFAESRIRRE TIAAEDILHD
360 370 380 390 400
MGAISVMSSD SQAMGRVGEV IIRTWQCANK MKLQRGILEG DNEHNDNERI
410 420 430 440 450
KRYVAKYTIN PAIAHGISHE VGSVEKGKLA DLVLWNPAFF GVKPALVMKS
460 470 480 490 500
GLVAYAPMGD INAAIPTPQP VHYRPMFACY GKAKFKSSMI FLSQASIEAG
510 520 530 540 550
VPEKLNLQSQ IGEVKGCRNI SKKSMIHNSY TPNIELDSQT YEVKADGVPL
560
VCEPATELPM AQRYFLF
Length:567
Mass (Da):61,174
Last modified:March 15, 2005 - v1
Checksum:iE20D54BBDD94ABA5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000020 Genomic DNA. Translation: AAW85168.1.
RefSeqiWP_011261402.1. NC_006840.2.
YP_204056.1. NC_006840.2.

Genome annotation databases

EnsemblBacteriaiAAW85168; AAW85168; VF_0673.
GeneIDi3277826.
KEGGivfi:VF_0673.
PATRICi20111890. VBIVibFis127983_0666.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000020 Genomic DNA. Translation: AAW85168.1.
RefSeqiWP_011261402.1. NC_006840.2.
YP_204056.1. NC_006840.2.

3D structure databases

ProteinModelPortaliQ5E728.
SMRiQ5E728. Positions 2-567.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi312309.VF_0673.

Protein family/group databases

MEROPSiM38.982.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW85168; AAW85168; VF_0673.
GeneIDi3277826.
KEGGivfi:VF_0673.
PATRICi20111890. VBIVibFis127983_0666.

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
HOGENOMiHOG000075064.
KOiK01428.
OMAiFDSHIHF.
OrthoDBiEOG6ND0GM.

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.
BioCyciAFIS312309:GIWP-708-MONOMER.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700601 / ES114.

Entry informationi

Entry nameiURE1_VIBF1
AccessioniPrimary (citable) accession number: Q5E728
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: March 15, 2005
Last modified: November 11, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.