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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Vibrio fischeri (strain ATCC 700601 / ES114)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei621 – 6211ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAFIS312309:GIWP-788-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:VF_0753
OrganismiVibrio fischeri (strain ATCC 700601 / ES114)
Taxonomic identifieri312309 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
ProteomesiUP000000537 Componenti: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 858858Leucine--tRNA ligasePRO_1000009462Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi312309.VF_0753.

Structurei

3D structure databases

ProteinModelPortaliQ5E6U8.
SMRiQ5E6U8. Positions 228-413.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi618 – 6225"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5E6U8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEQYNPQDL EQKIQKHWDD NKTFVVTEDA NKEKFYCLSM FPYPSGRLHM
60 70 80 90 100
GHVRNYTIGD VVSRYQRLQG KNVMQPIGWD AFGLPAENAA VKNKTAPAPW
110 120 130 140 150
TYENIEYMKN QLKLLGFGYD WNREFATCTP EYYRWEQEFF TKLYNKGLVY
160 170 180 190 200
KKTSSVNWCP NDQTVLANEQ VEDGCCWRCD TPVEQKKIPQ WFIKITEYAQ
210 220 230 240 250
ELLDDLDNLD GWPEMVKTMQ RNWIGRSEGV ELSFAVNGET SPLEVYTTRP
260 270 280 290 300
DTLMGVTYVG IAAGHPLAEK ASQNNPELAA FVEECRNTKV AEAELATMEK
310 320 330 340 350
KGMDTGLRAI HPLNGREVPV FVANFVLMDY GTGAVMAVPA HDQRDFEFAT
360 370 380 390 400
KYGLDIIPVI KPEDGSDLDV SEAAYTEKGV LFDSGEFDGL AFQEAFDAIA
410 420 430 440 450
AKLEAEGKGK KTVNFRLRDW GVSRQRYWGA PIPMVTTEDG EVHPVPADQL
460 470 480 490 500
PVILPEDVVM DGVTSPIKAD KEWAKTTFNG EPALRETDTF DTFMESSWYY
510 520 530 540 550
ARYCSPQADD ILDPEKANYW LPVDQYIGGI EHACMHLLYS RFFHKLLRDA
560 570 580 590 600
GYVTSDEPFK QLLCQGMVLA DAFYYTNDKG GKEWVAPTDV TIERDGKGRI
610 620 630 640 650
EKAIDDQGRE VEHSGMIKMS KSKNNGIDPQ EMVDKYGADT VRLFMMFASP
660 670 680 690 700
ADMTLEWQES GVEGANRFLK RVWKLVHEHT NKGTTEALDT SSLTGDQKAL
710 720 730 740 750
RRDVHKTIAK VSDDIGRRQT FNTAIAAIME LMNKLNKAPQ ESAQDRALLD
760 770 780 790 800
EALKAVVAML YPMTPHASFA MWEALGESDL DSATWPTFDE NALVEDEKTI
810 820 830 840 850
VVMINGKLRA KLVVAADATE EHVRELGLKD ENAMKFLDGL TIRKVIYVPG

KLLNIVAN
Length:858
Mass (Da):97,019
Last modified:March 15, 2005 - v1
Checksum:i289398A66A4F8101
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000020 Genomic DNA. Translation: AAW85248.1.
RefSeqiYP_204136.1. NC_006840.2.

Genome annotation databases

EnsemblBacteriaiAAW85248; AAW85248; VF_0753.
GeneIDi3277409.
KEGGivfi:VF_0753.
PATRICi20112050. VBIVibFis127983_0746.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000020 Genomic DNA. Translation: AAW85248.1.
RefSeqiYP_204136.1. NC_006840.2.

3D structure databases

ProteinModelPortaliQ5E6U8.
SMRiQ5E6U8. Positions 228-413.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi312309.VF_0753.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW85248; AAW85248; VF_0753.
GeneIDi3277409.
KEGGivfi:VF_0753.
PATRICi20112050. VBIVibFis127983_0746.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciAFIS312309:GIWP-788-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700601 / ES114.

Entry informationi

Entry nameiSYL_VIBF1
AccessioniPrimary (citable) accession number: Q5E6U8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 15, 2005
Last modified: April 1, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.