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Protein

Release factor glutamine methyltransferase

Gene

prmC

Organism
Vibrio fischeri (strain ATCC 700601 / ES114)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei144 – 1441S-adenosyl-L-methionineUniRule annotation
Binding sitei172 – 1721S-adenosyl-L-methionineUniRule annotation
Binding sitei188 – 1881S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAFIS312309:GIWP-817-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Release factor glutamine methyltransferaseUniRule annotation (EC:2.1.1.297UniRule annotation)
Short name:
RF MTaseUniRule annotation
Alternative name(s):
N5-glutamine methyltransferase PrmCUniRule annotation
Protein-(glutamine-N5) MTase PrmCUniRule annotation
Protein-glutamine N-methyltransferase PrmCUniRule annotation
Gene namesi
Name:prmCUniRule annotation
Ordered Locus Names:VF_0769
OrganismiVibrio fischeri (strain ATCC 700601 / ES114)
Taxonomic identifieri312309 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
Proteomesi
  • UP000000537 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 284284Release factor glutamine methyltransferasePRO_0000414549Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi312309.VF_0769.

Structurei

3D structure databases

ProteinModelPortaliQ5E6T2.
SMRiQ5E6T2. Positions 9-280.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni121 – 1255S-adenosyl-L-methionine bindingUniRule annotation
Regioni188 – 1914Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EQY. Bacteria.
COG2890. LUCA.
HOGENOMiHOG000076274.
KOiK02493.
OMAiSVDAKVM.
OrthoDBiPOG091H02CJ.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_02126. RF_methyltr_PrmC. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR025714. Methyltranfer_dom.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF13847. Methyltransf_31. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5E6T2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSIEALLK RSIQSLTLEG SDSPQVDAAV LLCHVLDKPR SYLLTWPEKI
60 70 80 90 100
VSDEELGNFN ALLERRLAGE PIAYIVGYRE FWSLPLKVSP TTLIPRPDTE
110 120 130 140 150
RLVEVALDHL TPNAQSILDL GTGTGAIALA IASEMPTLNV IGVDYQDDAV
160 170 180 190 200
ELAKGNAKIN HINNVEFRQG SWFEPISLSD KFDIIVSNPP YIDGNDPHLS
210 220 230 240 250
EGDVRFEPQT ALVAEQNGFS DLIHIMQHGR EYLLNGGWLM MEHGFEQGEQ
260 270 280
LRHFFEEHGY INVKTEQDYA GNDRVTLGQW VVDN
Length:284
Mass (Da):31,750
Last modified:March 15, 2005 - v1
Checksum:i70BFC412CCABC492
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000020 Genomic DNA. Translation: AAW85264.1.
RefSeqiWP_011261470.1. NC_006840.2.
YP_204152.1. NC_006840.2.

Genome annotation databases

EnsemblBacteriaiAAW85264; AAW85264; VF_0769.
GeneIDi3277424.
KEGGivfi:VF_0769.
PATRICi20112108. VBIVibFis127983_0761.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000020 Genomic DNA. Translation: AAW85264.1.
RefSeqiWP_011261470.1. NC_006840.2.
YP_204152.1. NC_006840.2.

3D structure databases

ProteinModelPortaliQ5E6T2.
SMRiQ5E6T2. Positions 9-280.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi312309.VF_0769.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW85264; AAW85264; VF_0769.
GeneIDi3277424.
KEGGivfi:VF_0769.
PATRICi20112108. VBIVibFis127983_0761.

Phylogenomic databases

eggNOGiENOG4105EQY. Bacteria.
COG2890. LUCA.
HOGENOMiHOG000076274.
KOiK02493.
OMAiSVDAKVM.
OrthoDBiPOG091H02CJ.

Enzyme and pathway databases

BioCyciAFIS312309:GIWP-817-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_02126. RF_methyltr_PrmC. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR025714. Methyltranfer_dom.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF13847. Methyltransf_31. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPRMC_VIBF1
AccessioniPrimary (citable) accession number: Q5E6T2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 14, 2011
Last sequence update: March 15, 2005
Last modified: September 7, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.