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Protein

Glutamate decarboxylase

Gene

gadA

Organism
Vibrio fischeri (strain ATCC 700601 / ES114)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the pyridoxal-dependent decarboxylation of glutamate to produce 4-aminobutanoate. Has weak activity with aspartate, but cannot complement an E. coli panD deletion mutant.1 Publication

Catalytic activityi

L-glutamate = 4-aminobutanoate + CO2.1 Publication

Cofactori

pyridoxal 5'-phosphate1 Publication

Kineticsi

kcat is 0.055 sec(-1) at 37 degrees Celsius.1 Publication
  1. KM=61.7 mM for glutamate (at 37 degrees Celsius)1 Publication

    GO - Molecular functioni

    • glutamate decarboxylase activity Source: UniProtKB
    • pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    • glutamate metabolic process Source: UniProtKB

    Keywordsi

    Molecular functionDecarboxylase, Lyase
    LigandPyridoxal phosphate

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate decarboxylase1 Publication (EC:4.1.1.151 Publication)
    Gene namesi
    Name:gadA1 Publication
    Ordered Locus Names:VF_1064Imported
    OrganismiVibrio fischeri (strain ATCC 700601 / ES114)
    Taxonomic identifieri312309 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
    Proteomesi
    • UP000000537 Componenti: Chromosome I

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004408731 – 464Glutamate decarboxylaseAdd BLAST464

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei274N6-(pyridoxal phosphate)lysineBy similarity1

    Interactioni

    Protein-protein interaction databases

    STRINGi312309.VF_1064.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5E5Y7.
    SMRiQ5E5Y7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the group II decarboxylase family.Curated

    Phylogenomic databases

    eggNOGiENOG4105CVK. Bacteria.
    COG0076. LUCA.
    HOGENOMiHOG000070228.
    KOiK01580.
    OMAiYTGRLFT.
    OrthoDBiPOG091H06F5.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiView protein in InterPro
    IPR010107. Glutamate_decarboxylase.
    IPR002129. PyrdxlP-dep_de-COase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_sub2.
    PANTHERiPTHR43321. PTHR43321. 1 hit.
    PfamiView protein in Pfam
    PF00282. Pyridoxal_deC. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR01788. Glu-decarb-GAD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5E5Y7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPLHSKNAVR DDLLDDIYSS ADLSLSMPKY KMPEQEHDPR HAYQVIHDEL
    60 70 80 90 100
    MMDGNSRQNL ATFCQTWVED EVHKLMDECI DKNMIDKDEY PQTAELESRC
    110 120 130 140 150
    VHMLADLWNS PDAENTLGCS TTGSSEAAML GGMALKWAWR EKMKKLGKPT
    160 170 180 190 200
    DKPNMICGPV QVCWHKFARY WDIELREIPM EGDRLIMTPE EVIKRCDENT
    210 220 230 240 250
    IGVVPTLGVT FTCQYEPVKA VHEALDKLQE ETGLDIPMHI DAASGGFLAP
    260 270 280 290 300
    FCDPDLEWDF RLPRVKSINA SGHKFGLSPL GVGWVIWRDA SALHEDLIFN
    310 320 330 340 350
    VNYLGGNMPT FALNFSRPGG QIVAQYYNFL RLGKEGYRKI HQACYDTAVY
    360 370 380 390 400
    LSSEIEKLGM FEIIYDGKGG IPAMSWSLKE GVDPGFNLFD LSDRIRSRGW
    410 420 430 440 450
    QIAAYAMPPK REDLVIMRIL VRHGFSRDQA DLLVADLKHC VEFFAKHPIS
    460
    HGSDELESSG FNHG
    Length:464
    Mass (Da):52,582
    Last modified:March 15, 2005 - v1
    Checksum:i51E50550E542FD6B
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000020 Genomic DNA. Translation: AAW85559.1.
    RefSeqiWP_011261695.1. NC_006840.2.
    YP_204447.1. NC_006840.2.

    Genome annotation databases

    EnsemblBacteriaiAAW85559; AAW85559; VF_1064.
    GeneIDi3278475.
    KEGGivfi:VF_1064.
    PATRICifig|312309.11.peg.1067.

    Similar proteinsi

    Entry informationi

    Entry nameiGADA_VIBF1
    AccessioniPrimary (citable) accession number: Q5E5Y7
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2017
    Last sequence update: March 15, 2005
    Last modified: October 25, 2017
    This is version 85 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families