Reviewed, UniProtKB/Swiss-Prot Q5E4N9 (TPMT_VIBF1)

Names and origin

Protein names

Recommended name:
    Thiopurine S-methyltransferase
    EC=2.1.1.67
Alternative name(s):
    Thiopurine methyltransferase

Gene names

Name:	tpm
Ordered Locus Names:	VF_1512

Organism

Vibrio fischeri (strain ATCC 700601 / ES114) [Complete proteome] [HAMAP]

Taxonomic identifier

312309 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether. HAMAP MF_00812
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	Thiopurine S-methyltransferase HAMAP MF_00812		PRO_0000220134
Sites
	Binding site	10	1	S-adenosyl-L-methionine By similarity
	Binding site	45	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	66	1	S-adenosyl-L-methionine By similarity
	Binding site	121	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5E4N9-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 057A64451F42851C	FASTA	213	24,538
	10 20 30 40 50 60 MEHEFWQKKW ASNVIGFHLP DTNPILTQYW SALEPKRNET VFVPLCGKSM DLDWLAERHN 70 80 90 100 110 120 SVTGVELSQI AVRAFFAERL YTPTVTQLSS TLELYEFDEF TIYSGDYFVA PIEAADLIYD 130 140 150 160 170 180 RAALVALPKE MREEYVQVLR SRLKEGGRIL LVTLDYDQNE MAGPPFSVPE NEVQALFSGM 190 200 210 KITRLQRDEA DAEHPKIKKG LSRFAEEVWL IES

References

[1]

"Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congeners." Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R., Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E., Stevens A., Visick K., Whistler C., Greenberg E.P. Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005) [PubMed: 15703294] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000020 Genomic DNA. Translation: AAW86007.1.
RefSeq	YP_204895.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5E4N9.
Genome annotation databases
GeneID	3278795.
GenomeReviews	Gene locus VF_1512 in contig CP000020_GR.
KEGG	vfi:VF_1512.
NMPDR	fig|312309.3.peg.1512.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5E4N9.
OMA	PPFAVSP.
Enzyme and pathway databases
BioCyc	VFIS312309:VF1512-MON.
Family and domain databases
HAMAP	MF_00812. [Tree]
InterPro	IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_VIBF1

Accession

Primary (citable) accession number: Q5E4N9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	March 15, 2005
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)