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Q5E4J7 (NRFA_VIBF1) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome c-552
EC=1.7.2.2
Alternative name(s):
Ammonia-forming cytochrome c nitrite reductase
Short name=Cytochrome c nitrite reductase
Gene names
Name:nrfA
Ordered Locus Names:VF_1554
OrganismVibrio fischeri (strain ATCC 700601 / ES114)
Taxonomic identifier312309 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays a role in nitrite reduction By similarity. HAMAP MF_01182

Catalytic activity

NH3 + 2 H2O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H+. HAMAP MF_01182

Cofactor

Binds 1 calcium ion per monomer By similarity. HAMAP MF_01182

Binds 5 heme groups covalently per monomer By similarity.

Pathway

Nitrogen metabolism; nitrate reduction (assimilation). HAMAP MF_01182

Subcellular location

Periplasm By similarity HAMAP MF_01182.

Sequence similarities

Belongs to the cytochrome c-552 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 478451Cytochrome c-552 HAMAP MF_01182
PRO_0000268981

Sites

Metal binding911Iron (heme 3 axial ligand) By similarity
Metal binding1231Iron (heme 1 axial ligand) By similarity
Metal binding1611Iron (heme 2 axial ligand) By similarity
Metal binding2101Iron (heme 3 axial ligand) By similarity
Metal binding2121Calcium By similarity
Metal binding2131Calcium; via carbonyl oxygen By similarity
Metal binding2581Calcium; via carbonyl oxygen By similarity
Metal binding2601Calcium By similarity
Metal binding2721Iron (heme 5 axial ligand) By similarity
Metal binding2831Iron (heme 4 axial ligand) By similarity
Metal binding2981Iron (heme 2 axial ligand) By similarity
Metal binding3151Iron (heme 5 axial ligand) By similarity
Metal binding3901Iron (heme 4 axial ligand) By similarity
Binding site1191Heme 1 (covalent) By similarity
Binding site1221Heme 1 (covalent) By similarity
Binding site1571Heme 2 (covalent) By similarity
Binding site1601Heme 2 (covalent) By similarity
Binding site2061Heme 3 (covalent) By similarity
Binding site2091Heme 3 (covalent) By similarity
Binding site2131Substrate By similarity
Binding site2611Substrate By similarity
Binding site2791Heme 4 (covalent) By similarity
Binding site2821Heme 4 (covalent) By similarity
Binding site3111Heme 5 (covalent) By similarity
Binding site3141Heme 5 (covalent) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5E4J7 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 52C2A56228E0D09D

FASTA47853,447
        10         20         30         40         50         60 
MKKQWTRRSA AAIAMVTTLL LSSHSFAASE NNERVDPRNE AYAKDHADQY DSWRSTSDSV 

        70         80         90        100        110        120 
QIEDALAEDP NMVILWAGYG FAKDYNKARG HFYAVDDVRQ TLRTGGPTDE NSGPMPMACW 

       130        140        150        160        170        180 
SCKSPDVGRV IEEQGEDGYF SGKWARLGSE IQNPIGCADC HDTRSEEFKN GGPALALTKP 

       190        200        210        220        230        240 
HVERAMDAIG KPFGDQSRLD QQAAVCGQCH VEYYFTGKTK AVKFPWDKGT TVDEMEEYYD 

       250        260        270        280        290        300 
EIGFKDWTHK VSKAPMLKAQ HPGYETWRDG IHGKNKVTCV DCHMPKVTKE DGTVYTDHKV 

       310        320        330        340        350        360 
GNPFDRFEDT CANCHTQSKE MLQSVVATRK AQVLKMKLTA ERQIVAAHFE AGAAWDAGAT 

       370        380        390        400        410        420 
EKEMAPILQD IRHAQWRWDY AIASHGVHMH APEVALEVLG TAVDKAADAR TKLVRLLAKK 

       430        440        450        460        470 
GITEPVAIPD ISTKAAAQKA LGMDMEAMKA EKKHFLETVV PEWEEQAKER EARDYEPM

« Hide

References

[1]"Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congeners."
Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R., Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E., Stevens A., Visick K., Whistler C., Greenberg E.P.
Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005) [PubMed: 15703294] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700601 / ES114.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000020 Genomic DNA. Translation: AAW86049.1.
RefSeqYP_204937.1. NC_006840.2.

3D structure databases

HSSPHSSP built from PDB template 1FS7 based on UniProtKB Q9S1E5.
ProteinModelPortalQ5E4J7.
SMRQ5E4J7. Positions 35-469.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ5E4J7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3278834.
GenomeReviewsGene locus VF_1554 in contig CP000020_GR.
KEGGvfi:VF_1554.
NMPDRfig|312309.3.peg.1554.
PATRIC20113742. VBIVibFis127983_1572.

Phylogenomic databases

eggNOGCOG3303.
HOGENOMHBG488281.
OMAPFAKASK.
PhylomeDBQ5E4J7.
ProtClustDBPRK11125.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-13459.
VFIS312309:VF1554-MONOMER.

Family and domain databases

HAMAPMF_01182. Cytochrom_C552.
[Tree]
InterProIPR003321. Cyt_c552.
IPR017570. Cyt_c_NO2Rdtase_formate-dep.
IPR011031. Multihaem_cyt.
[Graphical view]
KOK03385.
PfamPF02335. Cytochrom_C552. 1 hit.
[Graphical view]
PIRSFPIRSF000243. Cyt_c552. 1 hit.
TIGRFAMsTIGR03152. Cyto_c552_HCOOH. 1 hit.
PROSITEPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNRFA_VIBF1
AccessionPrimary (citable) accession number: Q5E4J7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: March 15, 2005
Last modified: January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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