NAD-dependent malic enzyme - Vibrio fischeri (strain ATCC 700601 / ES114)

Contribute

Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q5E4J3 (MAO1_VIBF1)

Last modified June 16, 2009. Version 31. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38

Gene names

Name:	sfcA
Ordered Locus Names:	VF_1558

Organism

Vibrio fischeri (strain ATCC 700601 / ES114) [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD⁺ = pyruvate + CO₂ + NADH. HAMAP MF_01619
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

562

NAD-dependent malic enzyme HAMAP MF_01619

PRO_0000160235

Sites

Active site

1

Proton donor By similarity

Active site

1

Proton acceptor By similarity

Metal binding

1

Divalent metal cation By similarity

Metal binding

1

Divalent metal cation By similarity

Metal binding

1

Divalent metal cation By similarity

Binding site

1

NAD By similarity

Binding site

1

NAD By similarity

Binding site

1

NAD By similarity

Site

1

Important for activity By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q5E4J3-1 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: DD7737D7F1B11DFE
Show »

562

62,299

        10         20         30         40         50         60 
MNNNKRPLYI PYAGPALLST PLLNKGSAFS TTERKYFNLE GLLPEAIESI EEQTGRAYKQ 

        70         80         90        100        110        120 
YQNFENDMDK HIYLRNIQDT NETLFYRLVQ NHISEMMPII YTPTVGAACE NFSNIYRRGR 

       130        140        150        160        170        180 
GLFISYENKN RIDDLLNNAA NQNVKVIVVT DGERILGLGD QGIGGMGIPI GKLALYTACG 

       190        200        210        220        230        240 
GISPAHTLPI VLDVGTNNPQ RLADPMYMGW RHPRVTGDEY ADFVEDFIQA VQRRWPEALV 

       250        260        270        280        290        300 
QFEDFAQKNA MPLLERYKNR ICCFNDDIQG TAAVTVGSLL AACKAAGSSL SEQRVTFLGA 

       310        320        330        340        350        360 
GSAGCGIAEA IIAQMVSEGI SDAQARSQVY MVDRWGLLQE GMPNLLDFQQ RLVQKAENTK 

       370        380        390        400        410        420 
DWVSEEPNFS LVDVMRNAKP TVLIGVSGAP GLFSKEVIQE MHKHCERPIV FPLSNPTSRV 

       430        440        450        460        470        480 
EATPNDIIRW TDGQALVATG SPFDPVAHNG QTYPIAQCNN SFIFPGIGLG VLAIKATRVT 

       490        500        510        520        530        540 
DEMLMESSRA LAECSPLAIH GTGALLPPLE EIHSVSKRIA FAVAKKAIEQ GHALEITDEA 

       550        560 
LQQKIESYFW KPVYRRYKRT AF

References

[1]

"Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congeners."
Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R., Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E., Stevens A., Visick K., Whistler C., Greenberg E.P.
Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005) [PubMed: 15703294] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
	CP000020 Genomic DNA. Translation: AAW86053.1.
RefSeq	YP_204941.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3278798.
GenomeReviews	Gene locus VF_1558 in contig CP000020_GR.
KEGG	vfi:VF_1558.
NMPDR	fig\|312309.3.peg.1558.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5E4J3.
OMA	Q5E4J3. IQDNNET.
Enzyme and pathway databases
BioCyc	VFIS312309:VF1558-MON.
Family and domain databases
HAMAP	MF_01619. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_VIBF1

Accession

Primary (citable) accession number: Q5E4J3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	March 15, 2005
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents