Q5E4J3 (MAO1_VIBF1) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 51.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: NAD-dependent malic enzyme Short name=NAD-ME EC=1.1.1.38 | ||||
| Gene names |
| ||||
| Organism | Vibrio fischeri (strain ATCC 700601 / ES114) | ||||
| Taxonomic identifier | 312309 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio |
Protein attributes
| Sequence length | 562 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Catalytic activity | (S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619 |
| Cofactor | Divalent metal cations. Prefers magnesium or manganese By similarity. |
| Subunit structure | Homotetramer By similarity. HAMAP MF_01619 |
| Sequence similarities | Belongs to the malic enzymes family. |
Ontologies
| Keywords | |
|---|---|
| Ligand | Metal-binding NAD |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | malate metabolic process Inferred from electronic annotation. Source: InterPro |
| Molecular function | NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activityInferred from electronic annotation. Source: EC metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 562 | 562 | NAD-dependent malic enzyme HAMAP MF_01619 | PRO_0000160235 | |||||
Sites | |||||||||
| Active site | 101 | 1 | Proton donor By similarity | ||||||
| Active site | 172 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 243 | 1 | Divalent metal cation By similarity | ||||||
| Metal binding | 244 | 1 | Divalent metal cation By similarity | ||||||
| Metal binding | 267 | 1 | Divalent metal cation By similarity | ||||||
| Binding site | 154 | 1 | NAD By similarity | ||||||
| Binding site | 267 | 1 | NAD By similarity | ||||||
| Binding site | 415 | 1 | NAD By similarity | ||||||
| Site | 267 | 1 | Important for activity By similarity | ||||||
Sequences
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References
| [1] | "Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congeners." Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R., Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E., Stevens A., Visick K., Whistler C., Greenberg E.P. Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005) [PubMed: 15703294] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700601 / ES114. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | CP000020 Genomic DNA. Translation: AAW86053.1. |
| RefSeq | YP_204941.1. NC_006840.2. |
3D structure databases | |
| ProteinModelPortal | Q5E4J3. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q5E4J3. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 3278798. |
| GenomeReviews | Gene locus VF_1558 in contig CP000020_GR. |
| KEGG | vfi:VF_1558. |
| NMPDR | fig|312309.3.peg.1558. |
| PATRIC | 20113750. VBIVibFis127983_1576. |
Phylogenomic databases | |
| eggNOG | COG0281. |
| HOGENOM | HBG289821. |
| OMA | QDNNETL. |
| PhylomeDB | Q5E4J3. |
| ProtClustDB | PRK13529. |
Enzyme and pathway databases | |
| BioCyc | VFIS312309:VF1558-MONOMER. |
Family and domain databases | |
| HAMAP | MF_01619. NAD_malic_enz. [Tree] |
| InterPro | IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD-bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. IPR023667. NAD_malic_enz_proteobac. [Graphical view] |
| Gene3D | G3DSA:3.40.50.10380. G3DSA:3.40.50.10380. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| KO | K00027. |
| Pfam | PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view] |
| PIRSF | PIRSF000106. ME. 1 hit. |
| PRINTS | PR00072. MALOXRDTASE. |
| SMART | SM00919. Malic_M. 1 hit. [Graphical view] |
| PROSITE | PS00331. MALIC_ENZYMES. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MAO1_VIBF1 | ||||||||
| Accession | Primary (citable) accession number: Q5E4J3 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

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