Q5E4J3 (MAO1_VIBF1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NAD-dependent malic enzyme
Short name=NAD-ME
EC=1.1.1.38

Gene names

Name:	maeA
Ordered Locus Names:	VF_1558

Organism

Vibrio fischeri (strain ATCC 700601 / ES114)

Taxonomic identifier

312309 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity. HAMAP MF_01619
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	NAD-dependent malic enzyme HAMAP MF_01619		PRO_0000160235
Sites
	Active site	101	1	Proton donor By similarity
	Active site	172	1	Proton acceptor By similarity
	Metal binding	243	1	Divalent metal cation By similarity
	Metal binding	244	1	Divalent metal cation By similarity
	Metal binding	267	1	Divalent metal cation By similarity
	Binding site	154	1	NAD By similarity
	Binding site	267	1	NAD By similarity
	Binding site	415	1	NAD By similarity
	Site	267	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5E4J3 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: DD7737D7F1B11DFE	FASTA	562	62,299
	10 20 30 40 50 60 MNNNKRPLYI PYAGPALLST PLLNKGSAFS TTERKYFNLE GLLPEAIESI EEQTGRAYKQ 70 80 90 100 110 120 YQNFENDMDK HIYLRNIQDT NETLFYRLVQ NHISEMMPII YTPTVGAACE NFSNIYRRGR 130 140 150 160 170 180 GLFISYENKN RIDDLLNNAA NQNVKVIVVT DGERILGLGD QGIGGMGIPI GKLALYTACG 190 200 210 220 230 240 GISPAHTLPI VLDVGTNNPQ RLADPMYMGW RHPRVTGDEY ADFVEDFIQA VQRRWPEALV 250 260 270 280 290 300 QFEDFAQKNA MPLLERYKNR ICCFNDDIQG TAAVTVGSLL AACKAAGSSL SEQRVTFLGA 310 320 330 340 350 360 GSAGCGIAEA IIAQMVSEGI SDAQARSQVY MVDRWGLLQE GMPNLLDFQQ RLVQKAENTK 370 380 390 400 410 420 DWVSEEPNFS LVDVMRNAKP TVLIGVSGAP GLFSKEVIQE MHKHCERPIV FPLSNPTSRV 430 440 450 460 470 480 EATPNDIIRW TDGQALVATG SPFDPVAHNG QTYPIAQCNN SFIFPGIGLG VLAIKATRVT 490 500 510 520 530 540 DEMLMESSRA LAECSPLAIH GTGALLPPLE EIHSVSKRIA FAVAKKAIEQ GHALEITDEA 550 560 LQQKIESYFW KPVYRRYKRT AF

References

[1]

"Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congeners." Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R., Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E., Stevens A., Visick K., Whistler C., Greenberg E.P. Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005) [PubMed: 15703294] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700601 / ES114.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000020 Genomic DNA. Translation: AAW86053.1.
RefSeq	YP_204941.1. NC_006840.2.
3D structure databases
ProteinModelPortal	Q5E4J3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5E4J3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3278798.
GenomeReviews	Gene locus VF_1558 in contig CP000020_GR.
KEGG	vfi:VF_1558.
NMPDR	fig|312309.3.peg.1558.
PATRIC	20113750. VBIVibFis127983_1576.
Phylogenomic databases
eggNOG	COG0281.
HOGENOM	HBG289821.
OMA	QDNNETL.
PhylomeDB	Q5E4J3.
ProtClustDB	PRK13529.
Enzyme and pathway databases
BioCyc	VFIS312309:VF1558-MONOMER.
Family and domain databases
HAMAP	MF_01619. NAD_malic_enz. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD-bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. IPR023667. NAD_malic_enz_proteobac. [Graphical view]
Gene3D	G3DSA:3.40.50.10380. G3DSA:3.40.50.10380. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00027.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PIRSF	PIRSF000106. ME. 1 hit.
PRINTS	PR00072. MALOXRDTASE.
SMART	SM00919. Malic_M. 1 hit. [Graphical view]
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_VIBF1

Accession

Primary (citable) accession number: Q5E4J3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program