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Q5E3U1

- FADJ_VIBF1

UniProt

Q5E3U1 - FADJ_VIBF1

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Protein

Fatty acid oxidation complex subunit alpha

Gene
fadJ, VF_1810
Organism
Vibrio fischeri (strain ATCC 700601 / ES114)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities By similarity.UniRule annotation

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei119 – 1191Important for catalytic activity By similarity
Sitei141 – 1411Important for catalytic activity By similarity

GO - Molecular functioni

  1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-HAMAP
  2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-HAMAP
  3. enoyl-CoA hydratase activity Source: UniProtKB-HAMAP
  4. NAD binding Source: InterPro

GO - Biological processi

  1. fatty acid beta-oxidation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid degradation, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciAFIS312309:GIWP-1914-MONOMER.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alpha
Including the following 2 domains:
Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase (EC:4.2.1.17, EC:5.1.2.3)
3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.35)
Gene namesi
Name:fadJ
Ordered Locus Names:VF_1810
OrganismiVibrio fischeri (strain ATCC 700601 / ES114)
Taxonomic identifieri312309 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
ProteomesiUP000000537: Chromosome I

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 687687Fatty acid oxidation complex subunit alphaUniRule annotationPRO_0000109311Add
BLAST

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI) By similarity.

Protein-protein interaction databases

STRINGi312309.VF_1810.

Structurei

3D structure databases

ProteinModelPortaliQ5E3U1.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 191191Enoyl-CoA hydratase By similarityAdd
BLAST
Regioni307 – 6873813-hydroxyacyl-CoA dehydrogenase By similarityAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Phylogenomic databases

eggNOGiCOG1250.
HOGENOMiHOG000261346.
KOiK01782.
OMAiPFRYMDT.
OrthoDBiEOG6M9F0M.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 2 hits.
HAMAPiMF_01617. FadJ.
InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR018376. Enoyl-CoA_hyd/isom_CS.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02440. FadJ. 1 hit.
PROSITEiPS00067. 3HCDH. 1 hit.
PS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5E3U1-1 [UniParc]FASTAAdd to Basket

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MKNTSAFAWT KDDEQIAWLT IDVPNEKMNT LQAAFAEQVT QVLDEIEEQQ    50
AHIKGLVIQS GKPDNFIAGA DINMIANCQN ASEAQALAEK GQHLFQRIED 100
LPFATVAAIH GPCLGGGLEL ALACDYRVCS DDNKTKLGLP EVQLGLLPGS 150
GGTQRLPRLI GLLPSLDIIL TGKQLRPKTA LKLGVVDASV PHTILSRIAA 200
DFALKKKAKR KLTAKEWGLS RNPLGRNVIF SQAEKQAQKK ARGNYPAIAA 250
ILDCIEHGLD KGMKKGLQRE AEQFARLAMT PESAALRSLF FAMTEMKKEK 300
GSDAEPKSID YVGVLGGGLM GGGIAHVSIA KAKKKVTIKD INNDGLLNAY 350
QYHYQRLDTL RKRRIISKAQ LQQQMLQLTG VTEFDGFKKL DVVVEAVFED 400
LNLKQEMVKA VQEQGKEDVI FATNTSSLPI GQIAEGAQKP ENIVGLHYFS 450
PVEKMPLVEV IPHATTSDET ISTVVALAKQ QGKTPIVVKD SAGFYVNRIL 500
APYMNEAARL LLAGEPIEVL DEALLDFGFP VGPISLLDEV GVDIGAKIMP 550
ILEAELGDRF RSPDVFQTLI DDKRLGKKTK RGFYVYKGKK KEPDQEVYTL 600
LNIKPQSQLS KNEIAMRCVL PMLAEAKRCL DEGIIASERD GDIGAIFGIG 650
FPPFLGGPFT YMNTLGEEKL ATLMRNYADK YGDRFIE 687
Length:687
Mass (Da):75,373
Last modified:December 6, 2005 - v2
Checksum:i5C85F16B06D6DDC0
GO

Sequence cautioni

The sequence AAW86305.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000020 Genomic DNA. Translation: AAW86305.1. Different initiation.
RefSeqiYP_205193.1. NC_006840.2.

Genome annotation databases

EnsemblBacteriaiAAW86305; AAW86305; VF_1810.
GeneIDi3279029.
KEGGivfi:VF_1810.
PATRICi20114314. VBIVibFis127983_1838.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000020 Genomic DNA. Translation: AAW86305.1 . Different initiation.
RefSeqi YP_205193.1. NC_006840.2.

3D structure databases

ProteinModelPortali Q5E3U1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 312309.VF_1810.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAW86305 ; AAW86305 ; VF_1810 .
GeneIDi 3279029.
KEGGi vfi:VF_1810.
PATRICi 20114314. VBIVibFis127983_1838.

Phylogenomic databases

eggNOGi COG1250.
HOGENOMi HOG000261346.
KOi K01782.
OMAi PFRYMDT.
OrthoDBi EOG6M9F0M.

Enzyme and pathway databases

UniPathwayi UPA00659 .
BioCyci AFIS312309:GIWP-1914-MONOMER.

Family and domain databases

Gene3Di 1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 2 hits.
HAMAPi MF_01617. FadJ.
InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR018376. Enoyl-CoA_hyd/isom_CS.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view ]
SUPFAMi SSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsi TIGR02440. FadJ. 1 hit.
PROSITEi PS00067. 3HCDH. 1 hit.
PS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700601 / ES114.

Entry informationi

Entry nameiFADJ_VIBF1
AccessioniPrimary (citable) accession number: Q5E3U1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: June 11, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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