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Q5E3J6 (NAPA_VIBF1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:VF_1905
OrganismVibrio fischeri (strain ATCC 700601 / ES114) [Reference proteome] [HAMAP]
Taxonomic identifier312309 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio

Protein attributes

Sequence length829 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929Tat-type signal Potential
Chain30 – 829800Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000046010

Regions

Domain41 – 97574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding481Iron-sulfur (4Fe-4S) By similarity
Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding551Iron-sulfur (4Fe-4S) By similarity
Metal binding831Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5E3J6 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 6EAD417D6C0B0826

FASTA82992,594
        10         20         30         40         50         60 
MKMTRRAFVK ANAAASAAAV AGVTLPASAT NLIVSSDQTK IKWDKAPCRF CGTGCSVLVG 

        70         80         90        100        110        120 
TQNGRVVATQ GDPEAPVNKG LNCIKGYFLS KIMYGKDRVQ TPMLRMKDGQ YNKDGDFAPV 

       130        140        150        160        170        180 
SWDVALDTMA EKWKAALKKH GPTGVGMFGS GQWTVMEGYA AVKLMKAGFR SNNIDPNARH 

       190        200        210        220        230        240 
CMASAVGAFM RTFGIDEPMG CYDDFENADS FVLWGSNMAE MHPVLWTRIS DRRLSHPHVK 

       250        260        270        280        290        300 
VNVLSTYYHR SFELADKGYI FEPQSDLAIA NFIANYIIQN NAVNWDFVNK HTNFKQATTD 

       310        320        330        340        350        360 
IGYGLRDDDP LQMEAANPNS GAMSSISFEE YKKSVAPYTA QKASEMSGVS EEDLITLAKQ 

       370        380        390        400        410        420 
YADPKTKVMS LWTMGMNQHT RGVWMNSLVY NIHLLTGKIA TPGNSPFSLT GQPSACGTAR 

       430        440        450        460        470        480 
EVGTFSHRLP ADMVVANPKH RAISEKIWKL PEGTLNGKPG AHAVVQDRML KDGKINAYWV 

       490        500        510        520        530        540 
MCNNNMQAGP NINTERLPGY RNPENFIVCS DPYPTATAQA ADLILPTAMW VEKEGAYGNA 

       550        560        570        580        590        600 
ERRTQAWYQQ VQAKGEAKSD LWQIMEFSKR FKVEEVWGEE LVAKAPEYRG KTMYDILFKN 

       610        620        630        640        650        660 
GQVDAFPLSE AQELNDDAKA QGFYVQKGLF EEYASFGRGH GHDLAPYDTY HTVRGLRWPV 

       670        680        690        700        710        720 
VDGKETLWRF KEGSDPYAKK GSDWDFYGKP DGKALIISAP YEAPPEVPND EFDMWLCTGR 

       730        740        750        760        770        780 
VLEHWHTGTM TRRVPELYKA VPDALCYIHP ADAKKRNLRR GDEVLISNKR GEVRVRVETR 

       790        800        810        820 
GRNRPPEGLV FVPFFDARIL INKLILDATD PLSKQTDFKK CPVKITKIA 

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References

[1]"Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congeners."
Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R., Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E., Stevens A., Visick K., Whistler C., Greenberg E.P.
Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700601 / ES114.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000020 Genomic DNA. Translation: AAW86400.1.
RefSeqYP_205288.1. NC_006840.2.

3D structure databases

ProteinModelPortalQ5E3J6.
SMRQ5E3J6. Positions 41-827.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING312309.VF_1905.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAW86400; AAW86400; VF_1905.
GeneID3279209.
KEGGvfi:VF_1905.
PATRIC20114502. VBIVibFis127983_1932.

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAEYRGKTM.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycAFIS312309:GIWP-2007-MONOMER.
MetaCyc:MONOMER-13447.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_VIBF1
AccessionPrimary (citable) accession number: Q5E3J6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: March 15, 2005
Last modified: May 14, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families