Reviewed, UniProtKB/Swiss-Prot Q5E3J6 (NAPA_VIBF1)

Names and origin

Protein names

Recommended name:
    Periplasmic nitrate reductase
    EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	VF_1905

Organism

Vibrio fischeri (strain ATCC 700601 / ES114) [Complete proteome] [HAMAP]

Taxonomic identifier

312309 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	829 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP MF_01630
Cofactor	Binds 1 4Fe-4S cluster By similarity. Binds 1 molybdenum ion per subunit By similarity. Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity.
Subunit structure	Interacts with napB By similarity.
Subcellular location	Periplasm By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Tat-type signal Potential
	Chain	30 – 829	800	Periplasmic nitrate reductase HAMAP MF_01630		PRO_0000046010
Sites
	Metal binding	48	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	51	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	55	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	83	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5E3J6-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 6EAD417D6C0B0826	FASTA	829	92,594
	10 20 30 40 50 60 MKMTRRAFVK ANAAASAAAV AGVTLPASAT NLIVSSDQTK IKWDKAPCRF CGTGCSVLVG 70 80 90 100 110 120 TQNGRVVATQ GDPEAPVNKG LNCIKGYFLS KIMYGKDRVQ TPMLRMKDGQ YNKDGDFAPV 130 140 150 160 170 180 SWDVALDTMA EKWKAALKKH GPTGVGMFGS GQWTVMEGYA AVKLMKAGFR SNNIDPNARH 190 200 210 220 230 240 CMASAVGAFM RTFGIDEPMG CYDDFENADS FVLWGSNMAE MHPVLWTRIS DRRLSHPHVK 250 260 270 280 290 300 VNVLSTYYHR SFELADKGYI FEPQSDLAIA NFIANYIIQN NAVNWDFVNK HTNFKQATTD 310 320 330 340 350 360 IGYGLRDDDP LQMEAANPNS GAMSSISFEE YKKSVAPYTA QKASEMSGVS EEDLITLAKQ 370 380 390 400 410 420 YADPKTKVMS LWTMGMNQHT RGVWMNSLVY NIHLLTGKIA TPGNSPFSLT GQPSACGTAR 430 440 450 460 470 480 EVGTFSHRLP ADMVVANPKH RAISEKIWKL PEGTLNGKPG AHAVVQDRML KDGKINAYWV 490 500 510 520 530 540 MCNNNMQAGP NINTERLPGY RNPENFIVCS DPYPTATAQA ADLILPTAMW VEKEGAYGNA 550 560 570 580 590 600 ERRTQAWYQQ VQAKGEAKSD LWQIMEFSKR FKVEEVWGEE LVAKAPEYRG KTMYDILFKN 610 620 630 640 650 660 GQVDAFPLSE AQELNDDAKA QGFYVQKGLF EEYASFGRGH GHDLAPYDTY HTVRGLRWPV 670 680 690 700 710 720 VDGKETLWRF KEGSDPYAKK GSDWDFYGKP DGKALIISAP YEAPPEVPND EFDMWLCTGR 730 740 750 760 770 780 VLEHWHTGTM TRRVPELYKA VPDALCYIHP ADAKKRNLRR GDEVLISNKR GEVRVRVETR 790 800 810 820 GRNRPPEGLV FVPFFDARIL INKLILDATD PLSKQTDFKK CPVKITKIA

References

[1]

"Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congeners." Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R., Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E., Stevens A., Visick K., Whistler C., Greenberg E.P. Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005) [PubMed: 15703294] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000020 Genomic DNA. Translation: AAW86400.1.
RefSeq	YP_205288.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3279209.
GenomeReviews	Gene locus VF_1905 in contig CP000020_GR.
KEGG	vfi:VF_1905.
NMPDR	fig|312309.3.peg.1905.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5E3J6.
OMA	Q5E3J6. ERRTQAW.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-13447. VFIS312309:VF1905-MON.
Family and domain databases
HAMAP	MF_01630. [Tree]
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006657. MPT_dinuc_bd. IPR010051. NO3_reductase_lsu_periplasm. IPR006311. Tat. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_VIBF1

Accession

Primary (citable) accession number: Q5E3J6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	March 15, 2005
Last modified:	June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)