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Q5E314

- RNC_VIBF1

UniProt

Q5E314 - RNC_VIBF1

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Protein
Ribonuclease 3
Gene
rnc, VF_2087
Organism
Vibrio fischeri (strain ATCC 700601 / ES114)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism By similarity.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mg2+ By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi40 – 401Magnesium By similarity
Active sitei44 – 441 Reviewed prediction
Metal bindingi113 – 1131Magnesium By similarity
Active sitei116 – 1161 By similarity
Metal bindingi116 – 1161Magnesium By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. rRNA binding Source: UniProtKB-KW
  3. ribonuclease III activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA processing Source: UniProtKB-HAMAP
  2. rRNA catabolic process Source: InterPro
  3. rRNA processing Source: UniProtKB-HAMAP
  4. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

mRNA processing, rRNA processing, tRNA processing

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciAFIS312309:GIWP-2198-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3 (EC:3.1.26.3)
Alternative name(s):
Ribonuclease III
Short name:
RNase III
Gene namesi
Name:rnc
Ordered Locus Names:VF_2087
OrganismiVibrio fischeri (strain ATCC 700601 / ES114)
Taxonomic identifieri312309 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
ProteomesiUP000000537: Chromosome I

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 223223Ribonuclease 3UniRule annotation
PRO_0000228601Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi312309.VF_2087.

Structurei

3D structure databases

ProteinModelPortaliQ5E314.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 127123RNase III
Add
BLAST
Domaini154 – 22370DRBM
Add
BLAST

Sequence similaritiesi

Contains 1 RNase III domain.

Phylogenomic databases

eggNOGiCOG0571.
HOGENOMiHOG000246809.
KOiK03685.
OMAiHRFPKVN.
OrthoDBiEOG6T1WVS.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERiPTHR11207. PTHR11207. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5E314-1 [UniParc]FASTAAdd to Basket

« Hide

MNSSLQRLEK KIGYQFKDES FLKLALTHRS ANGTHNERLE FLGDSILSFV    50
VADDLYHRFP KVDEGDMSRM RATLVRGHTL AELGREFQLG DYLYLGPGEL 100
KSGGFRRDSI LADAVEAIIG AIYLDSDTET VRGIILSWYN TRLESIEPGV 150
SQKDPKTRLQ EYLQGRRKPL PTYTVTKIKG EAHNQEFTIE CIVAGLDKPV 200
IGKGSSRRKA EQSAADIALG QLN 223
Length:223
Mass (Da):24,974
Last modified:March 15, 2005 - v1
Checksum:iC52BAA78ADA66467
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000020 Genomic DNA. Translation: AAW86582.1.
RefSeqiYP_205470.1. NC_006840.2.

Genome annotation databases

EnsemblBacteriaiAAW86582; AAW86582; VF_2087.
GeneIDi3279279.
KEGGivfi:VF_2087.
PATRICi20114896. VBIVibFis127983_2129.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000020 Genomic DNA. Translation: AAW86582.1 .
RefSeqi YP_205470.1. NC_006840.2.

3D structure databases

ProteinModelPortali Q5E314.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 312309.VF_2087.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAW86582 ; AAW86582 ; VF_2087 .
GeneIDi 3279279.
KEGGi vfi:VF_2087.
PATRICi 20114896. VBIVibFis127983_2129.

Phylogenomic databases

eggNOGi COG0571.
HOGENOMi HOG000246809.
KOi K03685.
OMAi HRFPKVN.
OrthoDBi EOG6T1WVS.

Enzyme and pathway databases

BioCyci AFIS312309:GIWP-2198-MONOMER.

Family and domain databases

Gene3Di 1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPi MF_00104. RNase_III.
InterProi IPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view ]
PANTHERi PTHR11207. PTHR11207. 1 hit.
Pfami PF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view ]
SMARTi SM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view ]
SUPFAMi SSF69065. SSF69065. 1 hit.
TIGRFAMsi TIGR02191. RNaseIII. 1 hit.
PROSITEi PS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700601 / ES114.

Entry informationi

Entry nameiRNC_VIBF1
AccessioniPrimary (citable) accession number: Q5E314
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: March 15, 2005
Last modified: September 3, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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