Q5E2W6 (GSA_VIBF1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutase
Short name=GSA
EC=5.4.3.8

Alternative name(s):
Glutamate-1-semialdehyde aminotransferase
Short name=GSA-AT

Gene names

Name:	hemL
Ordered Locus Names:	VF_2135

Organism

Vibrio fischeri (strain ATCC 700601 / ES114) [Reference proteome] [HAMAP]

Taxonomic identifier

312309 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio › Vibrio fischeri

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-4-amino-5-oxopentanoate = 5-aminolevulinate. HAMAP-Rule MF_00375
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2. HAMAP-Rule MF_00375
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00375.
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: HAMAP protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutamate-1-semialdehyde 2,1-aminomutase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transaminase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	Glutamate-1-semialdehyde 2,1-aminomutase HAMAP-Rule MF_00375		PRO_0000120464
Amino acid modifications
	Modified residue	265	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5E2W6 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: AC2C04C6A0B46FFF	FASTA	431	45,935
	10 20 30 40 50 60 MTKSAELFAK AQDKIPGGVN SPVRAFAGVG GSPIFVERAD GPLIFDADGK AYIDYVGSWG 70 80 90 100 110 120 PMILGHNHAA IREAVISAAQ RGLSFGAPTE TEITMAELVS ELVPSMEQVR MVSSGTEATM 130 140 150 160 170 180 SAIRLARGYT GRDKIMKFEG CYHGHADSLL VKAGSGALTL GQPSSPGVPA DFAKYTLTAT 190 200 210 220 230 240 FNDLDSVREL FAVNKGEIAC IIVEPVAGNM NCIPPVEGFH EGLRQICDEE GALLIFDEVM 250 260 270 280 290 300 TGFRVAENCA QGYYNIKPDL TTLGKVIGGG MPVGAFGGRK EVMQYIAPTG PVYQAGTLSG 310 320 330 340 350 360 NPVAMAAGFA CLKVLTEEGN EKRLADTTRH LANGFKELAN KHGIPMVVNQ VGGMFGFFFT 370 380 390 400 410 420 DQETVTSYAD VAKCDIERFK RFFHLMLKKG VYLAPSAFEA SFTSLAHGPK EIEATLEAAD 430 QCFAIIASEA K

References

[1]

"Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congeners." Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R., Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E., Stevens A., Visick K., Whistler C., Greenberg E.P. Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700601 / ES114.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000020 Genomic DNA. Translation: AAW86630.1.
RefSeq	YP_205518.1. NC_006840.2.
3D structure databases
ProteinModelPortal	Q5E2W6.
SMR	Q5E2W6. Positions 2-421.
ModBase	Search...
Protein-protein interaction databases
STRING	312309.VF_2135.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAW86630; AAW86630; VF_2135.
GeneID	3279385.
KEGG	vfi:VF_2135.
PATRIC	20115002. VBIVibFis127983_2177.
Phylogenomic databases
eggNOG	COG0001.
HOGENOM	HOG000020210.
KO	K01845.
OMA	VEMIRMT.
ProtClustDB	PRK00062.
Enzyme and pathway databases
BioCyc	AFIS312309:GIWP-2253-MONOMER.
UniPathway	UPA00251; UER00317.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 2 hits.
HAMAP	MF_00375. HemL_aminotrans_3.
InterPro	IPR004639. 4pyrrol_synth_GluAld_NH2Trfase. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
PANTHER	PTHR11986. PTHR11986. 1 hit. PTHR11986:SF5. PTHR11986:SF5. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00713. hemL. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSA_VIBF1

Accession

Primary (citable) accession number: Q5E2W6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program