Q5E2G9 (ASTD_VIBF1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-succinylglutamate 5-semialdehyde dehydrogenase
EC=1.2.1.71

Alternative name(s):
Succinylglutamic semialdehyde dehydrogenase
Short name=SGSD

Gene names

Name:	astD
Ordered Locus Names:	VF_2282

Organism

Vibrio fischeri (strain ATCC 700601 / ES114)

Taxonomic identifier

312309 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate By similarity. HAMAP MF_01174
Catalytic activity	N-succinyl-L-glutamate 5-semialdehyde + NAD+ + H2O = N-succinyl-L-glutamate + NADH. HAMAP MF_01174
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 4/5. HAMAP MF_01174
Sequence similarities	Belongs to the aldehyde dehydrogenase family. AstD subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	succinylglutamate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 485	485	N-succinylglutamate 5-semialdehyde dehydrogenase HAMAP MF_01174		PRO_0000262432
Regions
	Nucleotide binding	220 – 225	6	NAD By similarity
Sites
	Active site	243	1	By similarity
	Active site	278	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5E2G9 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 05C440F5066DFA4B	FASTA	485	52,319
	10 20 30 40 50 60 MTHWIAGDWV LGEGETIQSL SPYNSEVVWK GESATEAQVE SAVNAARNAF IEWKKLPFEN 70 80 90 100 110 120 RQAIVERFAE LVKENADSIA EVIAKETGKP FWETKTEAGA MVGKIAISIR AYHERTPYKE 130 140 150 160 170 180 REAAGNKIVL RHRPLGVMAV FGPYNFPGHL PNGHIVPALL AGNTVVLKPS EQTPWTSEVI 190 200 210 220 230 240 MKLWQKSGLP KGVINLVQGA RATGEALANT KGIDGLLFTG SANTGHILHR QFAGDTGKML 250 260 270 280 290 300 ALEMGGNNPM VISKAFGDLD ATVYTIVQSA FISAGQRCTC ARRLYVPEGK QGDALLARLV 310 320 330 340 350 360 DVTKNIHVDQ PFSETTPFMG PQISIAAAEF ILNAQKKLQA LGGESLLEAR SLGHAFVSPG 370 380 390 400 410 420 IIDATNVAEL PDEEYFGPLL QVIRYTTLAQ AVELANDTRY GLSAGLVSTD DSEWDYFVEH 430 440 450 460 470 480 IRSGIVNRNR QLTGASGDAP FGGPGASGNL KPSAYYAADY CAYPMASMEG DACEIPEQLS PGLTL

References

[1]

"Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congeners." Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R., Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E., Stevens A., Visick K., Whistler C., Greenberg E.P. Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005) [PubMed: 15703294] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700601 / ES114.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000020 Genomic DNA. Translation: AAW86777.1.
RefSeq	YP_205665.1. NC_006840.2.
3D structure databases
ProteinModelPortal	Q5E2G9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5E2G9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3278335.
GenomeReviews	Gene locus VF_2282 in contig CP000020_GR.
KEGG	vfi:VF_2282.
NMPDR	fig|312309.3.peg.2282.
PATRIC	20115308. VBIVibFis127983_2320.
Phylogenomic databases
eggNOG	COG1012.
HOGENOM	HBG752218.
OMA	DISITAY.
PhylomeDB	Q5E2G9.
ProtClustDB	PRK09457.
Enzyme and pathway databases
BioCyc	VFIS312309:VF2282-MONOMER.
Family and domain databases
HAMAP	MF_01174. Aldedh_AstD. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR017649. SuccinylGlu_semiald_DH_AstD. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
KO	K06447.
PANTHER	PTHR11699:SF24. PTHR11699:SF24. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR03240. Arg_catab_astD. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASTD_VIBF1

Accession

Primary (citable) accession number: Q5E2G9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program