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Q5E2G5

- SYW_VIBF1

UniProt

Q5E2G5 - SYW_VIBF1

Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Vibrio fischeri (strain ATCC 700601 / ES114)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (15 Mar 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei201 – 2011ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. tryptophan-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tryptophanyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciAFIS312309:GIWP-2411-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
    Alternative name(s):
    Tryptophanyl-tRNA synthetaseUniRule annotation
    Short name:
    TrpRSUniRule annotation
    Gene namesi
    Name:trpSUniRule annotation
    Ordered Locus Names:VF_2286
    OrganismiVibrio fischeri (strain ATCC 700601 / ES114)
    Taxonomic identifieri312309 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
    ProteomesiUP000000537: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 338338Tryptophan--tRNA ligasePRO_0000136706Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi312309.VF_2286.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5E2G5.
    SMRiQ5E2G5. Positions 5-335.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi12 – 209"HIGH" region
    Motifi198 – 2025"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0180.
    HOGENOMiHOG000059940.
    KOiK01867.
    OMAiLLNCYTY.
    OrthoDBiEOG686NJQ.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    HAMAPiMF_00140_B. Trp_tRNA_synth_B.
    InterProiIPR002305. aa-tRNA-synth_Ic.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR002306. Trp-tRNA-ligase.
    IPR024109. Trp-tRNA-ligase_bac-type.
    [Graphical view]
    PANTHERiPTHR10055. PTHR10055. 1 hit.
    PfamiPF00579. tRNA-synt_1b. 1 hit.
    [Graphical view]
    PRINTSiPR01039. TRNASYNTHTRP.
    TIGRFAMsiTIGR00233. trpS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5E2G5-1 [UniParc]FASTAAdd to Basket

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    MSKPIVLSGV QPSGELSIGN YLGALRQWQQ MQDDYDCQYC VVDLHAITVR    50
    QDPKALHEAT LDALAICLAV GVDPKKSTLF VQSHVPEHAQ LGWLLNCYTQ 100
    MGELSRMTQF KDKSARHSND VNVGLFDYPV LMAADILLYG AHQVPVGSDQ 150
    KQHLELARDI ATRFNNIYSP EAPIFTVPEP YIPQVNARVM SLQDATKKMS 200
    KSDDNRKNVI TLLEEPKSIL KKINKAQTDA EMPPRIAHDW ENKAGISNLM 250
    GLYSAATGKT FEEIEAQYQG VEMYGPFKKD VGEAIVTMLE PIQEEYKRIR 300
    EDRAYMDAVM KAGAEKASER AAVTLKKAYE AVGFVTRP 338
    Length:338
    Mass (Da):37,794
    Last modified:March 15, 2005 - v1
    Checksum:iE41B630F30B88F5D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000020 Genomic DNA. Translation: AAW86781.1.
    RefSeqiYP_205669.1. NC_006840.2.

    Genome annotation databases

    EnsemblBacteriaiAAW86781; AAW86781; VF_2286.
    GeneIDi3279549.
    KEGGivfi:VF_2286.
    PATRICi20115316. VBIVibFis127983_2324.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000020 Genomic DNA. Translation: AAW86781.1 .
    RefSeqi YP_205669.1. NC_006840.2.

    3D structure databases

    ProteinModelPortali Q5E2G5.
    SMRi Q5E2G5. Positions 5-335.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 312309.VF_2286.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAW86781 ; AAW86781 ; VF_2286 .
    GeneIDi 3279549.
    KEGGi vfi:VF_2286.
    PATRICi 20115316. VBIVibFis127983_2324.

    Phylogenomic databases

    eggNOGi COG0180.
    HOGENOMi HOG000059940.
    KOi K01867.
    OMAi LLNCYTY.
    OrthoDBi EOG686NJQ.

    Enzyme and pathway databases

    BioCyci AFIS312309:GIWP-2411-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    HAMAPi MF_00140_B. Trp_tRNA_synth_B.
    InterProi IPR002305. aa-tRNA-synth_Ic.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR002306. Trp-tRNA-ligase.
    IPR024109. Trp-tRNA-ligase_bac-type.
    [Graphical view ]
    PANTHERi PTHR10055. PTHR10055. 1 hit.
    Pfami PF00579. tRNA-synt_1b. 1 hit.
    [Graphical view ]
    PRINTSi PR01039. TRNASYNTHTRP.
    TIGRFAMsi TIGR00233. trpS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700601 / ES114.

    Entry informationi

    Entry nameiSYW_VIBF1
    AccessioniPrimary (citable) accession number: Q5E2G5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 2, 2005
    Last sequence update: March 15, 2005
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3