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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Vibrio fischeri (strain ATCC 700601 / ES114)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of tryptophan to tRNA(Trp).UniRule annotation

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei135L-tryptophanUniRule annotation1
Binding sitei189ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 13ATPUniRule annotation3
Nucleotide bindingi19 – 20ATPUniRule annotation2
Nucleotide bindingi147 – 149ATPUniRule annotation3
Nucleotide bindingi198 – 202ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciVFIS312309:G1G40-2407-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotation
Ordered Locus Names:VF_2286
OrganismiVibrio fischeri (strain ATCC 700601 / ES114)
Taxonomic identifieri312309 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
Proteomesi
  • UP000000537 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001367061 – 338Tryptophan--tRNA ligaseAdd BLAST338

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi312309.VF_2286

Structurei

3D structure databases

ProteinModelPortaliQ5E2G5
SMRiQ5E2G5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi12 – 20"HIGH" regionUniRule annotation9
Motifi198 – 202"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C31 Bacteria
COG0180 LUCA
HOGENOMiHOG000059940
KOiK01867
OMAiQRMTQFK
OrthoDBiPOG091H00I9

Family and domain databases

CDDicd00806 TrpRS_core, 1 hit
Gene3Di3.40.50.620, 2 hits
HAMAPiMF_00140_B Trp_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR002306 Trp-tRNA-ligase
IPR024109 Trp-tRNA-ligase_bac-type
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PRINTSiPR01039 TRNASYNTHTRP
TIGRFAMsiTIGR00233 trpS, 1 hit

Sequencei

Sequence statusi: Complete.

Q5E2G5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKPIVLSGV QPSGELSIGN YLGALRQWQQ MQDDYDCQYC VVDLHAITVR
60 70 80 90 100
QDPKALHEAT LDALAICLAV GVDPKKSTLF VQSHVPEHAQ LGWLLNCYTQ
110 120 130 140 150
MGELSRMTQF KDKSARHSND VNVGLFDYPV LMAADILLYG AHQVPVGSDQ
160 170 180 190 200
KQHLELARDI ATRFNNIYSP EAPIFTVPEP YIPQVNARVM SLQDATKKMS
210 220 230 240 250
KSDDNRKNVI TLLEEPKSIL KKINKAQTDA EMPPRIAHDW ENKAGISNLM
260 270 280 290 300
GLYSAATGKT FEEIEAQYQG VEMYGPFKKD VGEAIVTMLE PIQEEYKRIR
310 320 330
EDRAYMDAVM KAGAEKASER AAVTLKKAYE AVGFVTRP
Length:338
Mass (Da):37,794
Last modified:March 15, 2005 - v1
Checksum:iE41B630F30B88F5D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000020 Genomic DNA Translation: AAW86781.1
RefSeqiWP_005421081.1, NC_006840.2
YP_205669.1, NC_006840.2

Genome annotation databases

EnsemblBacteriaiAAW86781; AAW86781; VF_2286
GeneIDi3279549
KEGGivfi:VF_2286
PATRICifig|312309.11.peg.2324

Similar proteinsi

Entry informationi

Entry nameiSYW_VIBF1
AccessioniPrimary (citable) accession number: Q5E2G5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 2, 2005
Last sequence update: March 15, 2005
Last modified: March 28, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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