Reviewed, UniProtKB/Swiss-Prot Q5E2E4 (ARGE_VIBF1)

Names and origin

Protein names

Recommended name:
    Acetylornithine deacetylase
      Short name=Acetylornithinase
      Short name=AO
    EC=3.5.1.16
Alternative name(s):
    N-acetylornithinase
      Short name=NAO

Gene names

Name:	argE
Ordered Locus Names:	VF_2307

Organism

Vibrio fischeri (strain ATCC 700601 / ES114) [Complete proteome] [HAMAP]

Taxonomic identifier

312309 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	N(2)-acetyl-L-ornithine + H2O = acetate + L-ornithine. HAMAP MF_01108
Cofactor	Binds 2 zinc or cobalt ions per subunit By similarity. Glutathione By similarity.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine from N(2)-acetyl-L-ornithine (linear): step 1/1. HAMAP MF_01108
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the peptidase M20A family. ArgE subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	Cobalt Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetylornithine deacetylase activity Inferred from electronic annotation. Source: HAMAP cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW metallopeptidase activity Inferred from electronic annotation. Source: InterPro protein dimerization activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Acetylornithine deacetylase HAMAP MF_01108		PRO_1000065065
Sites
	Active site	78	1	By similarity
	Active site	140	1	By similarity
	Metal binding	76	1	Cobalt or zinc 1 By similarity
	Metal binding	108	1	Cobalt or zinc 1 By similarity
	Metal binding	108	1	Cobalt or zinc 2 By similarity
	Metal binding	141	1	Cobalt or zinc 2 By similarity
	Metal binding	165	1	Cobalt or zinc 1 By similarity
	Metal binding	351	1	Cobalt or zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5E2E4-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: BAD7A5AA08F3EA8D	FASTA	378	41,840
	10 20 30 40 50 60 MKFPEFKDYY QQLISTSSIS STDSSWDEGN AEVINKLAQW CEDLGCEVEI EEIEKGKLNL 70 80 90 100 110 120 LAKLGSGEGG LLLAGHTDTV PYDEGRWNYE PHALTEANDR FYGLGTADMK GFFAFILEAI 130 140 150 160 170 180 KNINWKDQSK PLYILATCDE ETTMLGARHF ASNTKIQPDY CIIGEPTSLK PIRGHKGHVA 190 200 210 220 230 240 NAIRVTGKSG HSSDPAHGVN ALEIMNEIMF ALMTLKNKLV KEYHNPGFSI PYPTLNLGHI 250 260 270 280 290 300 HGGDSPNRIC GCCELHYDVR PLPGISLDGL DNMLRDALKE IEAKWPGRID ITPLHEPIPG 310 320 330 340 350 360 YECSADSPIV TSTADICGQD VETVNYCTEA PFLQDLCPTL VLGPGSIEQA HQPDEYLAFS 370 FIDPTISILS KLMYKHCF

References

[1]

"Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congeners." Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R., Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E., Stevens A., Visick K., Whistler C., Greenberg E.P. Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005) [PubMed: 15703294] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000020 Genomic DNA. Translation: AAW86802.1.
RefSeq	YP_205690.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5E2E4.
Protein family/group databases
MEROPS	M20.974.
Genome annotation databases
GeneID	3279490.
GenomeReviews	Gene locus VF_2307 in contig CP000020_GR.
KEGG	vfi:VF_2307.
NMPDR	fig|312309.3.peg.2307.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5E2E4.
OMA	QPLMILA.
Enzyme and pathway databases
BioCyc	VFIS312309:VF2307-MON.
Family and domain databases
HAMAP	MF_01108. [Tree]
InterPro	IPR010169. AcOrn-deacetyl. IPR001261. ArgE/DapE_CS. IPR002933. Peptidase_M20. IPR011650. Peptidase_M20_dimer. [Graphical view]
Pfam	PF07687. M20_dimer. 1 hit. PF01546. Peptidase_M20. 1 hit. [Graphical view]
TIGRFAMs	TIGR01892. AcOrn-deacetyl. 1 hit.
PROSITE	PS00758. ARGE_DAPE_CPG2_1. 1 hit. PS00759. ARGE_DAPE_CPG2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGE_VIBF1

Accession

Primary (citable) accession number: Q5E2E4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	March 15, 2005
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)