Reviewed, UniProtKB/Swiss-Prot Q5E212 (STHA_VIBF1)

Names and origin

Protein names

Recommended name:
    Soluble pyridine nucleotide transhydrogenase
      Short name=STH
    EC=1.6.1.1
Alternative name(s):
    NAD(P)(+) transhydrogenase [B-specific]

Gene names

Name:	sthA
Ordered Locus Names:	VF_2439

Organism

Vibrio fischeri (strain ATCC 700601 / ES114) [Complete proteome] [HAMAP]

Taxonomic identifier

312309 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation By similarity.
Catalytic activity	NADPH + NAD(+) = NADP(+) + NADH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NADP metabolic process Inferred from electronic annotation. Source: HAMAP cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NAD(P)+ transhydrogenase (B-specific) activity Inferred from electronic annotation. Source: HAMAP electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	Soluble pyridine nucleotide transhydrogenase		PRO_0000260245
Regions
	Nucleotide binding	41 – 50	10	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5E212-1 [UniParc]. Last modified November 28, 2006. Version 2. Checksum: A9AE438FF166DD15	FASTA	471	51,674
	10 20 30 40 50 60 MTNSTPIRST HFDAIVIGSG PGGEGAAMGL TKANLNVAII EREPSVGGGC THWGTIPSKA 70 80 90 100 110 120 LRHAVSRIIE FNSNPLYCKN NTSLHSTFSD ILGHAKSVID KQTRMRQGFY DRNQCSLIFG 130 140 150 160 170 180 EASFVEKNTV AVTAKDGSIE TYTADKFIIA TGSRPYRPEG INFNHSRVYD SDSILSLKHD 190 200 210 220 230 240 PRHIIIYGAG VIGSEYASIF RGLGVKVDLV NTRDRLLSFL DNEMSDALSY HFWNSGIVTR 250 260 270 280 290 300 NDENFEHIEA NDDGVIMHLE SGKKMKADCI LFANGRTGNT DKLNLSAVGL EADSRGQLKV 310 320 330 340 350 360 NDNYQTDVEH IYAVGDVIGY PSLASAAYDQ GRFTAQAITK GKAEARLIDH IPTGIYTIPE 370 380 390 400 410 420 ISSVGKTEQE LTAAKVPYEV GRSSFKHLAR AQIAGKDIGS LKILFHRETK EILGIHCFGE 430 440 450 460 470 RAAEIIHIGQ AIMEQKGEAN TIEYFVNTTF NYPTMAEAYR VAALNGLNRL F

References

[1]

"Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congeners." Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R., Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E., Stevens A., Visick K., Whistler C., Greenberg E.P. Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005) [PubMed: 15703294] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000020 Genomic DNA. Translation: AAW86934.1. Different initiation.
RefSeq	YP_205822.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3279648.
GenomeReviews	Gene locus VF_2439 in contig CP000020_GR.
KEGG	vfi:VF2439.
NMPDR	fig|312309.3.peg.2439.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5E212.
Enzyme and pathway databases
BioCyc	VFIS312309:VF2439-MON.
Family and domain databases
HAMAP	MF_00247. [Tree]
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR000815. Hg_reductase. IPR001100. Pyr_nuc-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE. PR00411. PNDRDTASEI.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

STHA_VIBF1

Accession

Primary (citable) accession number: Q5E212

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	November 28, 2006
Last modified:	November 25, 2008
This is version 28 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)