Reviewed, UniProtKB/Swiss-Prot Q5E1R3 (PURK_VIBF1)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole carboxylase ATPase subunit
    EC=4.1.1.21
Alternative name(s):
    AIR carboxylase
      Short name=AIRC

Gene names

Name:	purK
Ordered Locus Names:	VF_2538

Organism

Vibrio fischeri (strain ATCC 700601 / ES114) [Complete proteome] [HAMAP]

Taxonomic identifier

312309 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses an ATPase activity that is dependent on the presence of AIR (aminoimidazole ribonucleotide). The association of purK and purE produces an enzyme complex capable of converting AIR to CAIR efficiently under physiological condition By similarity.
Catalytic activity	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the purK/purT family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	Phosphoribosylaminoimidazole carboxylase ATPase subunit		PRO_0000075016
Regions
	Domain	97 – 288	192	ATP-grasp
	Nucleotide binding	175 – 178	4	ATP By similarity
	Nucleotide binding	258 – 259	2	ATP By similarity
Sites
	Binding site	93	1	ATP By similarity
	Binding site	133	1	ATP By similarity
	Binding site	144	1	ATP By similarity
	Binding site	183	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5E1R3-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 797F90E7489AD36F	FASTA	370	40,933
	10 20 30 40 50 60 MNVLILGSGQ LARMMSLASA PLNIDVIAYD VNSQQIVHPL TQQPIDLSLE QAIKQVDVIT 70 80 90 100 110 120 AEFEHIPHHI LDICSVSGKF QPTPDAIKAG GDRRLEKKLL DNAHVANAKY HNINTKQDFL 130 140 150 160 170 180 NAIDTVGIPM VLKSALGGYD GKGQWRLKDV SQVDSIWEEM DELIKSSPNQ AIVAEQFIPF 190 200 210 220 230 240 NREVSLIGAR NKNGQMVTYP LTENVHVDGV LALSTALEGT SNLQQQADEM FTAIADELNY 250 260 270 280 290 300 IGVLAIEFFD VDGTLLVNEI APRVHNSGHW TQQGTDICQF EMHIRAVCNL PLTGNQLLRS 310 320 330 340 350 360 TAMINILGED SLPDEILTMP NCHIHWYGKE KRAGRKMGHI NVCADSPEML QKELSKLANI 370 LDKQAFKALS

References

[1]

"Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congeners." Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R., Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E., Stevens A., Visick K., Whistler C., Greenberg E.P. Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005) [PubMed: 15703294] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000020 Genomic DNA. Translation: AAW87033.1.
RefSeq	YP_205921.1.
3D structure databases
SMR	Q5E1R3. Positions 1-362.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5E1R3.
Genome annotation databases
GeneID	3279691.
GenomeReviews	Gene locus VF_2538 in contig CP000020_GR.
KEGG	vfi:VF_2538.
NMPDR	fig|312309.3.peg.2538.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0026.
HOGENOM	HBG516369.
OMA	YDGKGQA.
PhylomeDB	Q5E1R3.
Enzyme and pathway databases
BioCyc	VFIS312309:VF2538-MONOMER.
Family and domain databases
InterPro	IPR005875. AIR_COase_ATPase-su. IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PANTHER	PTHR23047:SF1. PurK_ATP. 1 hit.
Pfam	PF02222. ATP-grasp. 1 hit. [Graphical view]
TIGRFAMs	TIGR01161. purK. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURK_VIBF1

Accession

Primary (citable) accession number: Q5E1R3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	March 15, 2005
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)