Reviewed, UniProtKB/Swiss-Prot Q5DZU8 (PANB2_VIBF1)

Names and origin

Protein names

Recommended name:
    3-methyl-2-oxobutanoate hydroxymethyltransferase 2
    EC=2.1.2.11
Alternative name(s):
    Ketopantoate hydroxymethyltransferase 2
      Short name=KPHMT 2

Gene names

Name:	panB2
Ordered Locus Names:	VF_A0628

Organism

Vibrio fischeri (strain ATCC 700601 / ES114) [Complete proteome] [HAMAP]

Taxonomic identifier

312309 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate. HAMAP MF_00156
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2. HAMAP MF_00156
Subunit structure	Homodecamer; pentamer of dimers By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the panB family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-methyl-2-oxobutanoate hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	3-methyl-2-oxobutanoate hydroxymethyltransferase 2 HAMAP MF_00156		PRO_0000297409
Regions
	Region	45 – 46	2	Alpha-ketoisovalerate binding By similarity
Sites
	Active site	181	1	Proton acceptor By similarity
	Metal binding	45	1	Magnesium By similarity
	Metal binding	84	1	Magnesium By similarity
	Metal binding	114	1	Magnesium By similarity
	Binding site	84	1	Alpha-ketoisovalerate By similarity
	Binding site	112	1	Alpha-ketoisovalerate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5DZU8-1 [UniParc]. Last modified July 24, 2007. Version 2. Checksum: 3BF5C3BBC17E463D	FASTA	263	28,601
	10 20 30 40 50 60 MKKITISDLN NWKEVGEKFA SITAYDASFA KIFEEQSMPV IIVGDSLGSV IQGKPTTLDV 70 80 90 100 110 120 SIEELVYHTK CVRAGSPNAF IISDLPFMSY HTPEAACGSA AKLMKAGANM VKIEGGSWLI 130 140 150 160 170 180 NTVKMLTERA VPVCAHLGLM PQAVNIYGGY KLQGKTEEQA TKMLENAKEL QRAGAQALIL 190 200 210 220 230 240 ECIPAKLARQ ITLELHIPVI GIGAGNDTDG QVLVMHDILG ISANYIPRFS RNFLAETGSI 250 260 EAAVKKYIDE VKQQTFPGEK HCF

References

[1]

"Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congeners." Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R., Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E., Stevens A., Visick K., Whistler C., Greenberg E.P. Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005) [PubMed: 15703294] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000021 Genomic DNA. Translation: AAW87698.1. Different initiation.
RefSeq	YP_206586.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5DZU8.
Genome annotation databases
GeneID	3280238.
GenomeReviews	Gene locus VF_A0628 in contig CP000021_GR.
KEGG	vfi:VF_A0628.
NMPDR	fig|312309.3.peg.3387.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5DZU8.
OMA	MSYLLDP.
Enzyme and pathway databases
BioCyc	VFIS312309:VFA0628-MON.
Family and domain databases
HAMAP	MF_00156. [Tree]
InterPro	IPR003700. Pantoate_hydroxy_MeTrfase. [Graphical view]
PANTHER	PTHR20881. Pantoate_transf. 1 hit.
Pfam	PF02548. Pantoate_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
TIGRFAMs	TIGR00222. panB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANB2_VIBF1

Accession

Primary (citable) accession number: Q5DZU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	July 24, 2007
Last modified:	November 3, 2009
This is version 29 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)