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Protein

Spermatogenesis-associated protein 13

Gene

Spata13

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA (By similarity). Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.By similarity1 Publication

Enzyme regulationi

Both the ABR and the SH3 domains contribute to maintaining the protein in an inhibited conformation by associating with the C-terminal tail. Binding of these domains to the C-terminal tail inhibits the activity of the protein by blocking a region that is required for its GEF activity (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
Spermatogenesis-associated protein 13
Alternative name(s):
APC-stimulated guanine nucleotide exchange factor 2
Short name:
Asef2
Gene namesi
Name:Spata13
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:104838. Spata13.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002784491 – 656Spermatogenesis-associated protein 13Add BLAST656

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei82PhosphoserineCombined sources1
Modified residuei118PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ5DU57.
PaxDbiQ5DU57.
PeptideAtlasiQ5DU57.
PRIDEiQ5DU57.

PTM databases

iPTMnetiQ5DU57.
PhosphoSitePlusiQ5DU57.

Expressioni

Tissue specificityi

Expression is aberrantly enhanced in most colorectal tumors.1 Publication

Gene expression databases

BgeeiENSMUSG00000021990.
CleanExiMM_SPATA13.

Interactioni

Subunit structurei

Interacts (via ABR and SH3 domain) with APC. The binding of APC enhances its GEF activity by relieving it from an autoinhibitory conformation, in which the ABR and SH3 domains are associated with the C-terminal tail. Interacts (via C-terminal tail) with PPP1R9B (via C-terminus). Interacts with RAC1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022566.

Structurei

3D structure databases

ProteinModelPortaliQ5DU57.
SMRiQ5DU57.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini151 – 210SH3PROSITE-ProRule annotationAdd BLAST60
Domaini244 – 428DHPROSITE-ProRule annotationAdd BLAST185
Domaini459 – 565PHPROSITE-ProRule annotationAdd BLAST107

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni102 – 154ABR (APC-binding region) domainBy similarityAdd BLAST53
Regioni565 – 656C-terminal tailBy similarityAdd BLAST92

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi24 – 27Poly-Arg4

Domaini

The C-terminal tail is required for its GEF activity.By similarity

Sequence similaritiesi

Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiENOG410ITS0. Eukaryota.
ENOG410XQDV. LUCA.
HOGENOMiHOG000237363.
HOVERGENiHBG050568.
InParanoidiQ5DU57.
KOiK05769.
PhylomeDBiQ5DU57.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5DU57-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHPASVTTTS QDPCAPSGSC RGGRRRRPIS VIGGVSFYGN TQVEDVENLL
60 70 80 90 100
VQPAARPPVP AHQVPPYKAV SARLRPFTFS QSTPIGLDRV GRRRQMKTSN
110 120 130 140 150
VSSDGGAESS ALVDDNGSEE DFSYEELCQA NPRYLQPGGE QLAINELISD
160 170 180 190 200
GSVVCAEALW DHVTMDDQEL GFKAGDVIQV LEASNKDWWW GRNEDKEAWF
210 220 230 240 250
PASFVRLRVN QEELPENCSS SHGEEQDEDT SKARHKHPES QQQMRTNVIQ
260 270 280 290 300
EIMNTERVYI KHLKDICEGY IRQCRKHTGM FTVAQLATIF GNIEDIYKFQ
310 320 330 340 350
RKFLKDLEKQ YNKEEPHLSE IGSCFLEHQE GFAIYSEYCN NHPGACVELS
360 370 380 390 400
NLMKHSKYRH FFEACRLLQQ MIDIALDGFL LTPVQKICKY PLQLAELLKY
410 420 430 440 450
TTQEHGDYNN IKAAYEAMKN VACLINERKR KLESIDKIAR WQVSIVGWEG
460 470 480 490 500
LDILDRSSEL IHSGELTKIT RQGKSQQRIF FLFDHQLVSC KKDLLRRDML
510 520 530 540 550
YYKGRMDMDE VELVDVEDGR DKDWSLSLRN AFKLVSKATD EVHLFCARKQ
560 570 580 590 600
EDKARWLQAY ADERRRVQED QQMGMEIPEN QKKLAMLNAQ KAGHGKSKGY
610 620 630 640 650
NSCPVAPPHQ SLPPLHQRHI TVPTSIPQQQ VFALAEPKRK PSIFWHTFHK

LTPFRK
Length:656
Mass (Da):75,257
Last modified:February 20, 2007 - v2
Checksum:iB85BB725EC3059E2
GO
Isoform 2 (identifier: Q5DU57-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-352: Missing.

Note: No experimental confirmation available.
Show »
Length:304
Mass (Da):35,590
Checksum:i3A9F9F9FCE19F896
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0232871 – 352Missing in isoform 2. 1 PublicationAdd BLAST352

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK158193 mRNA. Translation: BAE34401.1.
AC151835 Genomic DNA. No translation available.
AC114000 Genomic DNA. No translation available.
AK220313 mRNA. Translation: BAD90389.1.
RefSeqiNP_001028444.1. NM_001033272.2.
NP_001297523.1. NM_001310594.1.
UniGeneiMm.149776.

Genome annotation databases

GeneIDi219140.
KEGGimmu:219140.
UCSCiuc007ufe.2. mouse. [Q5DU57-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK158193 mRNA. Translation: BAE34401.1.
AC151835 Genomic DNA. No translation available.
AC114000 Genomic DNA. No translation available.
AK220313 mRNA. Translation: BAD90389.1.
RefSeqiNP_001028444.1. NM_001033272.2.
NP_001297523.1. NM_001310594.1.
UniGeneiMm.149776.

3D structure databases

ProteinModelPortaliQ5DU57.
SMRiQ5DU57.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022566.

PTM databases

iPTMnetiQ5DU57.
PhosphoSitePlusiQ5DU57.

Proteomic databases

EPDiQ5DU57.
PaxDbiQ5DU57.
PeptideAtlasiQ5DU57.
PRIDEiQ5DU57.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi219140.
KEGGimmu:219140.
UCSCiuc007ufe.2. mouse. [Q5DU57-1]

Organism-specific databases

CTDi221178.
MGIiMGI:104838. Spata13.

Phylogenomic databases

eggNOGiENOG410ITS0. Eukaryota.
ENOG410XQDV. LUCA.
HOGENOMiHOG000237363.
HOVERGENiHBG050568.
InParanoidiQ5DU57.
KOiK05769.
PhylomeDBiQ5DU57.

Miscellaneous databases

PROiQ5DU57.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021990.
CleanExiMM_SPATA13.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPT13_MOUSE
AccessioniPrimary (citable) accession number: Q5DU57
Secondary accession number(s): Q3TZ09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: February 20, 2007
Last modified: November 2, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.