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Protein

Volume-regulated anion channel subunit LRRC8B

Gene

Lrrc8b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes. The VRAC channel conducts iodide better than chloride and may also conduct organic osmolytes like taurine. Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Volume-regulated anion channel subunit LRRC8B
Alternative name(s):
Leucine-rich repeat-containing protein 8B
T-cell activation leucine repeat-rich protein
Short name:
TA-LRRP
Gene namesi
Name:Lrrc8b
Synonyms:Kiaa0231
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2141353. Lrrc8b.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity
  • Endoplasmic reticulum membrane By similarity

  • Note: In the absence of LRRC8A, resides primarily in a cytoplasmic compartment, probably the endoplasmic reticulum. Requires LRRC8A for expression at the cell membrane.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 25CytoplasmicSequence analysisAdd BLAST25
Transmembranei26 – 46HelicalSequence analysisAdd BLAST21
Topological domaini47 – 119ExtracellularSequence analysisAdd BLAST73
Transmembranei120 – 140HelicalSequence analysisAdd BLAST21
Topological domaini141 – 261CytoplasmicSequence analysisAdd BLAST121
Transmembranei262 – 282HelicalSequence analysisAdd BLAST21
Topological domaini283 – 307ExtracellularSequence analysisAdd BLAST25
Transmembranei308 – 328HelicalSequence analysisAdd BLAST21
Topological domaini329 – 803CytoplasmicSequence analysisAdd BLAST475

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000762461 – 803Volume-regulated anion channel subunit LRRC8BAdd BLAST803

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi78N-linked (GlcNAc...)Sequence analysis1
Modified residuei186PhosphoserineCombined sources1
Modified residuei196PhosphoserineCombined sources1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ5DU41.
PeptideAtlasiQ5DU41.
PRIDEiQ5DU41.

PTM databases

iPTMnetiQ5DU41.
PhosphoSitePlusiQ5DU41.

Expressioni

Gene expression databases

BgeeiENSMUSG00000070639.
CleanExiMM_LRRC8B.
GenevisibleiQ5DU41. MM.

Interactioni

Subunit structurei

Heterooligomer; heterooligomerizes with other LRRC8 proteins (LRRC8A, LRRC8C, LRRC8D and/or LRRC8E), possibly to form a heterohexamer (PubMed:24782309). In vivo, the subunit composition may depend primarily on expression levels, and heterooligomeric channels containing various proportions of the different LRRC8 proteins may coexist (Probable).Curated1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000108327.

Structurei

3D structure databases

ProteinModelPortaliQ5DU41.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati415 – 439LRR 1Add BLAST25
Repeati440 – 462LRR 2Add BLAST23
Repeati464 – 486LRR 3Add BLAST23
Repeati488 – 509LRR 4Add BLAST22
Repeati511 – 532LRR 5Add BLAST22
Repeati539 – 559LRR 6Add BLAST21
Repeati562 – 582LRR 7Add BLAST21
Repeati586 – 607LRR 8Add BLAST22
Repeati609 – 630LRR 9Add BLAST22
Repeati634 – 655LRR 10Add BLAST22
Repeati657 – 678LRR 11Add BLAST22
Repeati680 – 701LRR 12Add BLAST22
Repeati703 – 724LRR 13Add BLAST22
Repeati726 – 747LRR 14Add BLAST22
Repeati749 – 771LRR 15Add BLAST23

Sequence similaritiesi

Belongs to the LRRC8 family.Curated
Contains 15 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00840000129676.
HOGENOMiHOG000231806.
HOVERGENiHBG052360.
InParanoidiQ5DU41.
OMAiNSQDKVE.
OrthoDBiEOG091G01ER.
TreeFamiTF331443.

Family and domain databases

Gene3Di3.80.10.10. 3 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR021040. LRRC8_Pannexin-like.
[Graphical view]
PfamiPF13855. LRR_8. 2 hits.
PF12534. Pannexin_like. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 7 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 10 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5DU41-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MITLTELKCL ADAQSSYHIL KPWWDVFWYY ITLIMLLVAV LAGALQLTQS
60 70 80 90 100
RVLCCLPCKV EFDNQCAVPW DLLKGSENAS SNSGLLLPLP LRIQNDLHRQ
110 120 130 140 150
QYSYIDAVCY EKQLHWFAKF FPYLVLLHTL IFAACSNFWL HYPSTSSRLE
160 170 180 190 200
HFVSILHKCF DSPWTTRALS ETVAEQSVRP LKLSKSKTLL STSGGSADID
210 220 230 240 250
ASKQSLPYPQ PGLESPGIES PTSSVLDKKE GEQAKAIFEK VKRFRLHVEQ
260 270 280 290 300
RDIIYRVYLK QIIVKVILFV LIITYVPYFL SYITLEIDCS IDVQAFTGYK
310 320 330 340 350
RYQCVYSLAE IFKVLASFYV ILVMLYGLTS SYSLWWMLRS SLKQYSFEAL
360 370 380 390 400
REKSNYSDIP DVKNDFAFIL HLADQYDPLY SKRFSIFLSE VSENKLKQIN
410 420 430 440 450
LNNEWTVERL KSKLVKNSQD KVELHLFMLN GLPDNVFELT EMEVLSLELI
460 470 480 490 500
PEVKLPAAVA QLVNLRELHV YHSSLVVDHP ALAFLEENLR ILRLKFTEMG
510 520 530 540 550
KIPRWVFHLK NLKELYLSGC VLPEQLSSLH LEGFQDLKNL RTLYLKSSLS
560 570 580 590 600
RIPQVVTDLL PSLQKLSLDN EGSKLVVLNN LKKMVNLKSL ELLSCDLERI
610 620 630 640 650
PHSIFSLNNL HELDLKENNL KTVEEIISFQ HLPSLSCLKL WHNNIAYIPA
660 670 680 690 700
QIGALSNLEQ LFLGHNNIES LPLQLFLCTK LHYLDLSYNH LTFIPEEIQY
710 720 730 740 750
LTNLQYFAVT NNNIEMLPDG LFQCKKLQCL LLGRNSLTDL SPLVGELSNL
760 770 780 790 800
THLELTGNYL ETLPVELEGC QSLKRSCLIV EDSLLNSLPL PVAERLQTCL

DKC
Length:803
Mass (Da):92,186
Last modified:July 9, 2014 - v3
Checksum:i5E6E4DE4A5136613
GO
Isoform 2 (identifier: Q5DU41-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     169-180: Missing.

Show »
Length:791
Mass (Da):90,889
Checksum:i7B5BF7F8F10EF05D
GO

Sequence cautioni

The sequence BAD90398 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_055395169 – 180Missing in isoform 2. 1 PublicationAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220329 mRNA. Translation: BAD90398.1. Different initiation.
AC113980 Genomic DNA. No translation available.
AC138265 Genomic DNA. No translation available.
CH466529 Genomic DNA. Translation: EDL20199.1.
BC138877 mRNA. Translation: AAI38878.1.
BC138878 mRNA. Translation: AAI38879.1.
CCDSiCCDS39193.1. [Q5DU41-1]
RefSeqiNP_001028722.1. NM_001033550.2. [Q5DU41-1]
XP_011247810.1. XM_011249508.2. [Q5DU41-1]
UniGeneiMm.439781.
Mm.439828.

Genome annotation databases

EnsembliENSMUST00000112707; ENSMUSP00000108327; ENSMUSG00000070639. [Q5DU41-1]
GeneIDi433926.
KEGGimmu:433926.
UCSCiuc008yle.1. mouse. [Q5DU41-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220329 mRNA. Translation: BAD90398.1. Different initiation.
AC113980 Genomic DNA. No translation available.
AC138265 Genomic DNA. No translation available.
CH466529 Genomic DNA. Translation: EDL20199.1.
BC138877 mRNA. Translation: AAI38878.1.
BC138878 mRNA. Translation: AAI38879.1.
CCDSiCCDS39193.1. [Q5DU41-1]
RefSeqiNP_001028722.1. NM_001033550.2. [Q5DU41-1]
XP_011247810.1. XM_011249508.2. [Q5DU41-1]
UniGeneiMm.439781.
Mm.439828.

3D structure databases

ProteinModelPortaliQ5DU41.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000108327.

PTM databases

iPTMnetiQ5DU41.
PhosphoSitePlusiQ5DU41.

Proteomic databases

PaxDbiQ5DU41.
PeptideAtlasiQ5DU41.
PRIDEiQ5DU41.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000112707; ENSMUSP00000108327; ENSMUSG00000070639. [Q5DU41-1]
GeneIDi433926.
KEGGimmu:433926.
UCSCiuc008yle.1. mouse. [Q5DU41-1]

Organism-specific databases

CTDi23507.
MGIiMGI:2141353. Lrrc8b.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00840000129676.
HOGENOMiHOG000231806.
HOVERGENiHBG052360.
InParanoidiQ5DU41.
OMAiNSQDKVE.
OrthoDBiEOG091G01ER.
TreeFamiTF331443.

Miscellaneous databases

PROiQ5DU41.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000070639.
CleanExiMM_LRRC8B.
GenevisibleiQ5DU41. MM.

Family and domain databases

Gene3Di3.80.10.10. 3 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR021040. LRRC8_Pannexin-like.
[Graphical view]
PfamiPF13855. LRR_8. 2 hits.
PF12534. Pannexin_like. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 7 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 10 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLRC8B_MOUSE
AccessioniPrimary (citable) accession number: Q5DU41
Secondary accession number(s): B2RSI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: July 9, 2014
Last modified: November 30, 2016
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.