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Protein

Apoptotic protease-activating factor 1

Gene

Apaf1

Organism
Mus musculus (Mouse)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processApoptosisUniRule annotation
LigandATP-bindingUniRule annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Apoptotic protease-activating factor 1UniRule annotation
Short name:
APAF-1UniRule annotation
Gene namesi
Name:Apaf1Imported
Synonyms:mKIAA0413Imported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Organism-specific databases

MGIiMGI:1306796. Apaf1.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Proteomic databases

PaxDbiQ5DU30.
PRIDEiQ5DU30.

Expressioni

Gene expression databases

GenevisibleiQ5DU30. MM.

Interactioni

Subunit structurei

Monomer. Oligomerizes upon binding of cytochrome c and dATP.UniRule annotation

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020157.

Structurei

3D structure databases

ProteinModelPortaliQ5DU30.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 92CARDInterPro annotationAdd BLAST90
Domaini613 – 1215WD_REPEATS_REGIONInterPro annotationAdd BLAST603
Repeati613 – 654WDPROSITE-ProRule annotationAdd BLAST42
Repeati655 – 696WDPROSITE-ProRule annotationAdd BLAST42
Repeati697 – 740WDPROSITE-ProRule annotationAdd BLAST44
Repeati741 – 782WDPROSITE-ProRule annotationAdd BLAST42
Repeati880 – 912WDPROSITE-ProRule annotationAdd BLAST33
Repeati1001 – 1042WDPROSITE-ProRule annotationAdd BLAST42
Repeati1042 – 1082WDPROSITE-ProRule annotationAdd BLAST41
Repeati1083 – 1117WDPROSITE-ProRule annotationAdd BLAST35
Repeati1125 – 1166WDPROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

WD repeatPROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4155. Eukaryota.
KOG4658. Eukaryota.
ENOG410XP3K. LUCA.
HOGENOMiHOG000290173.
HOVERGENiHBG018730.
KOiK02084.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
2.130.10.10. 2 hits.
3.40.50.300. 1 hit.
InterProiView protein in InterPro
IPR017251. Apaf-1.
IPR024977. Apc4_WD40_dom.
IPR001315. CARD.
IPR011029. DEATH-like_dom.
IPR020472. G-protein_beta_WD-40_rep.
IPR002182. NB-ARC.
IPR027417. P-loop_NTPase.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
PfamiView protein in Pfam
PF12894. ANAPC4_WD40. 1 hit.
PF00619. CARD. 1 hit.
PF00931. NB-ARC. 1 hit.
PF00400. WD40. 8 hits.
PIRSFiPIRSF037646. Apop_pept_activating-1. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiView protein in SMART
SM00320. WD40. 13 hits.
SUPFAMiSSF47986. SSF47986. 1 hit.
SSF50978. SSF50978. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS50209. CARD. 1 hit.
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 9 hits.
PS50294. WD_REPEATS_REGION. 1 hit.

Sequencei

Sequence statusi: Fragment.

Q5DU30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
GTMDAKARNC LLQHREALEK DIKTSYIMDH MISNGVLSVI EEEKVKSQAT
60 70 80 90 100
QYQRAAALIK MILNKDNCAY ISFYNALLHE GYKDLAALLQ SGLPLVSSSS
110 120 130 140 150
GKDTDGGITS FVRTVLCEGG VPQRPVIFVT RKKLVHAIQQ KLWKLNGEPG
160 170 180 190 200
WVTIYGMAGC GKSVLAAEAV RDHSLLEGCF SGGVHWVSIG KQDKSGLLMK
210 220 230 240 250
LQNLCMRLDQ EESFSQRLPL NIEEAKDRLR VLMLRKHPRS LLILDDVWDP
260 270 280 290 300
WVLKAFDNQC QILLTTRDKS VTDSVMGPKH VVPVESGLGR EKGLEILSLF
310 320 330 340 350
VNMKKEDLPA EAHSIIKECK GSPLVVSLIG ALLRDFPNRW AYYLRQLQNK
360 370 380 390 400
QFKRIRKSSS YDYEALDEAM SISVEMLRED IKDYYTDLSI LQKDVKVPTK
410 420 430 440 450
VLCVLWDLET EEVEDILQEF VNKSLLFCNR NGKSFCYYLH DLQVDFLTEK
460 470 480 490 500
NRSQLQDLHR KMVTQFQRYY QPHTLSPDQE DCMYWYNFLA YHMASANMHK
510 520 530 540 550
ELCALMFSLD WIKAKTELVG PAHLIHEFVA YRHILDEKDC AVCENFQEFL
560 570 580 590 600
SLNGHLLGRQ PFPNIVQLGL CEPETSEVYR QAKLQAKQEG DTGRLYLEWI
610 620 630 640 650
NKKTIKNLSR LVVRPHTDAV YHACFSQDGQ RIASCGADKT LQVFKAETGE
660 670 680 690 700
KLLDIKAHED EVLCCAFSSD DSYIATCSAD KKVKIWDSAT GKLVHTYDEH
710 720 730 740 750
SEQVNCCHFT NKSNHLLLAT GSNDFFLKLW DLNQKECRNT MFGHTNSVNH
760 770 780 790 800
CRFSPDDELL ASCSADGTLR LWDVRSANER KSINVKRFFL SSEDPPEDVE
810 820 830 840 850
VIVKCCSWSA DGDKIIVAAK NKVLLFDIHT SGLLAEIHTG HHSTIQYCDF
860 870 880 890 900
SPYDHLAVIA LSQYCVELWN IDSRLKVADC RGHLSWVHGV MFSPDGSSFL
910 920 930 940 950
TASDDQTIRV WETKKVCKNS AIVLKQEIDV VFQENETMVL AVDNIRGLQL
960 970 980 990 1000
IAGKTGQIDY LPEAQVSCCC LSPHLEYVAF GDEDGAIKII ELPNNRVFSS
1010 1020 1030 1040 1050
GVGHKKAVRH IQFTADGKTL ISSSEDSVIQ VWNWQTGDYV FLQAHQETVK
1060 1070 1080 1090 1100
DFRLLQDSRL LSWSFDGTVK VWNVITGRIE RDFTCHQGTV LSCAISSDAT
1110 1120 1130 1140 1150
KFSSTSADKT AKIWSFDLLS PLHELKGHNG CVRCSAFSLD GILLATGDDN
1160 1170 1180 1190 1200
GEIRIWNVSD GQLLHSCAPI SVEEGTATHG GWVTDVCFSP DSKTLVSAGG
1210 1220 1230 1240 1250
YLKWWNVATG DSSQTFYTNG TNLKKIHVFP DFRTYVTVDN LGILYILQVL

E
Length:1,251
Mass (Da):141,221
Last modified:March 29, 2005 - v1
Checksum:iBB8474D2FC9357D6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei1Imported1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220340 mRNA. Translation: BAD90406.1.
RefSeqiNP_001036023.1. NM_001042558.1.
NP_001269876.1. NM_001282947.1.
NP_033814.2. NM_009684.2.
UniGeneiMm.220289.

Genome annotation databases

GeneIDi11783.
KEGGimmu:11783.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220340 mRNA. Translation: BAD90406.1.
RefSeqiNP_001036023.1. NM_001042558.1.
NP_001269876.1. NM_001282947.1.
NP_033814.2. NM_009684.2.
UniGeneiMm.220289.

3D structure databases

ProteinModelPortaliQ5DU30.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020157.

Proteomic databases

PaxDbiQ5DU30.
PRIDEiQ5DU30.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi11783.
KEGGimmu:11783.

Organism-specific databases

CTDi317.
MGIiMGI:1306796. Apaf1.

Phylogenomic databases

eggNOGiKOG4155. Eukaryota.
KOG4658. Eukaryota.
ENOG410XP3K. LUCA.
HOGENOMiHOG000290173.
HOVERGENiHBG018730.
KOiK02084.

Miscellaneous databases

ChiTaRSiApaf1. mouse.
SOURCEiSearch...

Gene expression databases

GenevisibleiQ5DU30. MM.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
2.130.10.10. 2 hits.
3.40.50.300. 1 hit.
InterProiView protein in InterPro
IPR017251. Apaf-1.
IPR024977. Apc4_WD40_dom.
IPR001315. CARD.
IPR011029. DEATH-like_dom.
IPR020472. G-protein_beta_WD-40_rep.
IPR002182. NB-ARC.
IPR027417. P-loop_NTPase.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
PfamiView protein in Pfam
PF12894. ANAPC4_WD40. 1 hit.
PF00619. CARD. 1 hit.
PF00931. NB-ARC. 1 hit.
PF00400. WD40. 8 hits.
PIRSFiPIRSF037646. Apop_pept_activating-1. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiView protein in SMART
SM00320. WD40. 13 hits.
SUPFAMiSSF47986. SSF47986. 1 hit.
SSF50978. SSF50978. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS50209. CARD. 1 hit.
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 9 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQ5DU30_MOUSE
AccessioniPrimary (citable) accession number: Q5DU30
Entry historyiIntegrated into UniProtKB/TrEMBL: March 29, 2005
Last sequence update: March 29, 2005
Last modified: February 15, 2017
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.