Q5DTU0 (AF1L2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Actin filament-associated protein 1-like 2
Short name=AFAP1-like protein 2

Gene names

Name:	Afap1l2
Synonyms:	Kiaa1914

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	825 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in a signaling cascade by enhancing the kinase activity of SRC. Contributes to SRC-regulated transcription activation By similarity.
Subunit structure	Interacts with SRC. Interacts with LCK when tyrosine phosphorylated By similarity.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Tyrosine phosphorylated (by SRC) By similarity.
Sequence similarities	Contains 2 PH domains.
Sequence caution	The sequence AAH31515.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC39879.1 differs from that shown. Reason: Frameshift at position 701. The sequence BAD90264.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	inflammatory response Inferred from electronic annotation. Source: Compara positive regulation of epidermal growth factor receptor signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of interleukin-8 production Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara regulation of interleukin-6 production Inferred from electronic annotation. Source: Compara regulation of mitotic cell cycle Inferred from electronic annotation. Source: Compara
Cellular_component	aggresome Inferred from electronic annotation. Source: Compara cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: Compara
Molecular_function	phospholipid binding Inferred from electronic annotation. Source: InterPro protein tyrosine kinase activator activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5DTU0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5DTU0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MERYKA → MERYKAQGCCCLVVQRRILQVSAS

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 825	825	Actin filament-associated protein 1-like 2		PRO_0000050806
Regions
	Domain	175 – 271	97	PH 1
	Domain	353 – 447	95	PH 2
	Coiled coil	657 – 754	98	Potential
Amino acid modifications
	Modified residue	56	1	Phosphotyrosine Ref.4
	Modified residue	345	1	Phosphothreonine Ref.5
	Modified residue	413	1	Phosphotyrosine Ref.4
Natural variations
	Alternative sequence	1 – 6	6	MERYKA → MERYKAQGCCCLVVQRRILQ VSAS in isoform 2.		VSP_014257
Experimental info
	Sequence conflict	124	1	Y → N in BAC39879. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 21, 2005. Version 2. Checksum: E0D929590FED4764	FASTA	825	92,175
	10 20 30 40 50 60 MERYKALEQL LTELDDFLKV LDQENLSSAA VLKKSGLSEL LRLYTKSSSS DEEYIYMNKV 70 80 90 100 110 120 SVNGEQNSAS PDKVPEEQGP LTNGEPSQHS SAPQKSLPDL PPPKMIPERK QPTVPKIESP 130 140 150 160 170 180 EGYYEEAEPF DRSINEDGEA VSSSYESYDE DENSKGKAAP YQWPSPEASI ELMRDARICA 190 200 210 220 230 240 FLWRKKWLGQ WAKQLCVIRD TRLLCYKSSK DHSPQLDVNL RGSSVVHKEK QVRKKGHKLK 250 260 270 280 290 300 ITPMNADVIV LGLQSKDQAE QWLRVIQEVS GLPSEGASEG NQYTPDAQRL NCQKPDIAEK 310 320 330 340 350 360 YLSAAEYGIT INGHPEIPET KDVKKKCSAG LKLSNLMNLG RKKSTSLEPP ERSLETSSYL 370 380 390 400 410 420 NVLVNSQWKS RWCFVRDSHL HFYQDRNRSK VAQQPLSLVG CDVLPDPSPD HLYSFRILHN 430 440 450 460 470 480 GEELAKLEAK SSEEMGHWLG LLLSESGSKT DPEELTYDYV DAERVSCIVS AAKTSLLLMQ 490 500 510 520 530 540 RKFSEPNTYI DGLPSRDCQD DLYDDVEVSE LIAVVEPAEE AAPAVDANSG SEPDRVYLDL 550 560 570 580 590 600 TPVKSFLHSS SEAQAQASLP AVPHQDDVAE TLTVDPKPGT TPEEPHTESP GDPEVQQRQP 610 620 630 640 650 660 EVQESSEPIE PTPRITMVKL QAEQQRISFP ANCPDTMASA PIAASPPVKE KLRVTSAEIK 670 680 690 700 710 720 LGKNRTEAEV KRYTEEKERL ERSKEEIRGH LAQLRREKRE LKETLLRCTD KGVLAKLEQT 730 740 750 760 770 780 LKKIDEECRM EESRRVDLEL SIMEVKDNLK KAEAGPVTLG TTVDTTHLDN MSPRPQPKAA 790 800 810 820 TPNPPPDSTP VNSASVLKNR PLSVMVTGKG TVLQKAKEWE KKGAS
	Isoform 2 [UniParc]. Checksum: B5C643244D3827BB	FASTA	843	94,120
	10 20 30 40 50 60 MERYKAQGCC CLVVQRRILQ VSASLEQLLT ELDDFLKVLD QENLSSAAVL KKSGLSELLR 70 80 90 100 110 120 LYTKSSSSDE EYIYMNKVSV NGEQNSASPD KVPEEQGPLT NGEPSQHSSA PQKSLPDLPP 130 140 150 160 170 180 PKMIPERKQP TVPKIESPEG YYEEAEPFDR SINEDGEAVS SSYESYDEDE NSKGKAAPYQ 190 200 210 220 230 240 WPSPEASIEL MRDARICAFL WRKKWLGQWA KQLCVIRDTR LLCYKSSKDH SPQLDVNLRG 250 260 270 280 290 300 SSVVHKEKQV RKKGHKLKIT PMNADVIVLG LQSKDQAEQW LRVIQEVSGL PSEGASEGNQ 310 320 330 340 350 360 YTPDAQRLNC QKPDIAEKYL SAAEYGITIN GHPEIPETKD VKKKCSAGLK LSNLMNLGRK 370 380 390 400 410 420 KSTSLEPPER SLETSSYLNV LVNSQWKSRW CFVRDSHLHF YQDRNRSKVA QQPLSLVGCD 430 440 450 460 470 480 VLPDPSPDHL YSFRILHNGE ELAKLEAKSS EEMGHWLGLL LSESGSKTDP EELTYDYVDA 490 500 510 520 530 540 ERVSCIVSAA KTSLLLMQRK FSEPNTYIDG LPSRDCQDDL YDDVEVSELI AVVEPAEEAA 550 560 570 580 590 600 PAVDANSGSE PDRVYLDLTP VKSFLHSSSE AQAQASLPAV PHQDDVAETL TVDPKPGTTP 610 620 630 640 650 660 EEPHTESPGD PEVQQRQPEV QESSEPIEPT PRITMVKLQA EQQRISFPAN CPDTMASAPI 670 680 690 700 710 720 AASPPVKEKL RVTSAEIKLG KNRTEAEVKR YTEEKERLER SKEEIRGHLA QLRREKRELK 730 740 750 760 770 780 ETLLRCTDKG VLAKLEQTLK KIDEECRMEE SRRVDLELSI MEVKDNLKKA EAGPVTLGTT 790 800 810 820 830 840 VDTTHLDNMS PRPQPKAATP NPPPDSTPVN SASVLKNRPL SVMVTGKGTV LQKAKEWEKK GAS

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Eye.
[2]	"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H. Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Fetal brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: FVB/N. Tissue: Mammary gland.
[4]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-56 AND TYR-413, MASS SPECTROMETRY. Tissue: Brain.
[5]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-345, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK087449 mRNA. Translation: BAC39879.1. Frameshift. AK220430 mRNA. Translation: BAD90264.1. Different initiation. BC031515 mRNA. Translation: AAH31515.1. Different initiation.
IPI	IPI00169899. IPI00608072.
RefSeq	NP_001171267.1. NM_001177796.1. NP_001171268.1. NM_001177797.1. NP_666214.1. NM_146102.2.
UniGene	Mm.226284.
3D structure databases
ProteinModelPortal	Q5DTU0.
SMR	Q5DTU0. Positions 354-443.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000107210.
PTM databases
PhosphoSite	Q5DTU0.
Proteomic databases
PaxDb	Q5DTU0.
PRIDE	Q5DTU0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000111584; ENSMUSP00000107210; ENSMUSG00000025083. ENSMUST00000118800; ENSMUSP00000113745; ENSMUSG00000025083. ENSMUST00000122359; ENSMUSP00000112387; ENSMUSG00000025083.
GeneID	226250.
KEGG	mmu:226250.
UCSC	uc008hzm.2. mouse. uc008hzn.2. mouse.
Organism-specific databases
CTD	84632.
MGI	MGI:2147658. Afap1l2.
Rouge	Search...
Phylogenomic databases
eggNOG	NOG308979.
GeneTree	ENSGT00390000003998.
HOGENOM	HOG000033832.
HOVERGEN	HBG106875.
OMA	KSAPYQW.
Gene expression databases
ArrayExpress	Q5DTU0.
Bgee	Q5DTU0.
CleanEx	MM_AFAP1L2.
Genevestigator	Q5DTU0.
GermOnline	ENSMUSG00000025083. Mus musculus.
Family and domain databases
Gene3D	2.30.29.30. 2 hits.
InterPro	IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. [Graphical view]
Pfam	PF00169. PH. 2 hits. [Graphical view]
SMART	SM00233. PH. 2 hits. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	378068.
SOURCE	Search...

Entry information

Entry name

AF1L2_MOUSE

Accession

Primary (citable) accession number: Q5DTU0
Secondary accession number(s): Q8BID1, Q8K2G0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 21, 2005
Last modified:	May 1, 2013
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program