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Protein

Actin filament-associated protein 1-like 2

Gene

Afap1l2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in a signaling cascade by enhancing the kinase activity of SRC. Contributes to SRC-regulated transcription activation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Actin filament-associated protein 1-like 2
Short name:
AFAP1-like protein 2
Gene namesi
Name:Afap1l2
Synonyms:Kiaa1914
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2147658. Afap1l2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 825825Actin filament-associated protein 1-like 2PRO_0000050806Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei56 – 561PhosphotyrosineCombined sources
Modified residuei113 – 1131PhosphothreonineCombined sources
Modified residuei408 – 4081PhosphoserineCombined sources
Modified residuei413 – 4131PhosphotyrosineCombined sources
Modified residuei484 – 4841PhosphoserineCombined sources

Post-translational modificationi

Tyrosine phosphorylated (by SRC).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ5DTU0.
PaxDbiQ5DTU0.
PRIDEiQ5DTU0.

PTM databases

iPTMnetiQ5DTU0.
PhosphoSiteiQ5DTU0.

Expressioni

Gene expression databases

BgeeiQ5DTU0.
CleanExiMM_AFAP1L2.
ExpressionAtlasiQ5DTU0. baseline and differential.
GenevisibleiQ5DTU0. MM.

Interactioni

Subunit structurei

Interacts with SRC. Interacts with LCK when tyrosine phosphorylated (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ5DTU0. 1 interaction.
MINTiMINT-4117315.
STRINGi10090.ENSMUSP00000107210.

Structurei

3D structure databases

ProteinModelPortaliQ5DTU0.
SMRiQ5DTU0. Positions 354-443.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini175 – 27197PH 1PROSITE-ProRule annotationAdd
BLAST
Domaini353 – 44795PH 2PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili657 – 75498Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 2 PH domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IEU6. Eukaryota.
ENOG4111CMN. LUCA.
GeneTreeiENSGT00390000003998.
HOGENOMiHOG000033832.
HOVERGENiHBG106875.
InParanoidiQ5DTU0.
OMAiQWKSRWC.
OrthoDBiEOG7KQ217.
PhylomeDBiQ5DTU0.
TreeFamiTF332622.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR030113. AFAP.
IPR030115. AFAP1L2.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR14338. PTHR14338. 1 hit.
PTHR14338:SF4. PTHR14338:SF4. 1 hit.
PfamiPF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS50003. PH_DOMAIN. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5DTU0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERYKALEQL LTELDDFLKV LDQENLSSAA VLKKSGLSEL LRLYTKSSSS
60 70 80 90 100
DEEYIYMNKV SVNGEQNSAS PDKVPEEQGP LTNGEPSQHS SAPQKSLPDL
110 120 130 140 150
PPPKMIPERK QPTVPKIESP EGYYEEAEPF DRSINEDGEA VSSSYESYDE
160 170 180 190 200
DENSKGKAAP YQWPSPEASI ELMRDARICA FLWRKKWLGQ WAKQLCVIRD
210 220 230 240 250
TRLLCYKSSK DHSPQLDVNL RGSSVVHKEK QVRKKGHKLK ITPMNADVIV
260 270 280 290 300
LGLQSKDQAE QWLRVIQEVS GLPSEGASEG NQYTPDAQRL NCQKPDIAEK
310 320 330 340 350
YLSAAEYGIT INGHPEIPET KDVKKKCSAG LKLSNLMNLG RKKSTSLEPP
360 370 380 390 400
ERSLETSSYL NVLVNSQWKS RWCFVRDSHL HFYQDRNRSK VAQQPLSLVG
410 420 430 440 450
CDVLPDPSPD HLYSFRILHN GEELAKLEAK SSEEMGHWLG LLLSESGSKT
460 470 480 490 500
DPEELTYDYV DAERVSCIVS AAKTSLLLMQ RKFSEPNTYI DGLPSRDCQD
510 520 530 540 550
DLYDDVEVSE LIAVVEPAEE AAPAVDANSG SEPDRVYLDL TPVKSFLHSS
560 570 580 590 600
SEAQAQASLP AVPHQDDVAE TLTVDPKPGT TPEEPHTESP GDPEVQQRQP
610 620 630 640 650
EVQESSEPIE PTPRITMVKL QAEQQRISFP ANCPDTMASA PIAASPPVKE
660 670 680 690 700
KLRVTSAEIK LGKNRTEAEV KRYTEEKERL ERSKEEIRGH LAQLRREKRE
710 720 730 740 750
LKETLLRCTD KGVLAKLEQT LKKIDEECRM EESRRVDLEL SIMEVKDNLK
760 770 780 790 800
KAEAGPVTLG TTVDTTHLDN MSPRPQPKAA TPNPPPDSTP VNSASVLKNR
810 820
PLSVMVTGKG TVLQKAKEWE KKGAS
Length:825
Mass (Da):92,175
Last modified:June 21, 2005 - v2
Checksum:iE0D929590FED4764
GO
Isoform 2 (identifier: Q5DTU0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MERYKA → MERYKAQGCCCLVVQRRILQVSAS

Show »
Length:843
Mass (Da):94,120
Checksum:iB5C643244D3827BB
GO

Sequence cautioni

The sequence AAH31515.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAC39879.1 differs from that shown. Reason: Frameshift at position 701. Curated
The sequence BAD90264.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti124 – 1241Y → N in BAC39879 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 66MERYKA → MERYKAQGCCCLVVQRRILQ VSAS in isoform 2. 2 PublicationsVSP_014257

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK087449 mRNA. Translation: BAC39879.1. Frameshift.
AK220430 mRNA. Translation: BAD90264.1. Different initiation.
BC031515 mRNA. Translation: AAH31515.1. Different initiation.
CCDSiCCDS50476.1. [Q5DTU0-1]
CCDS50477.1. [Q5DTU0-2]
RefSeqiNP_001171267.1. NM_001177796.1. [Q5DTU0-2]
NP_001171268.1. NM_001177797.1. [Q5DTU0-1]
NP_666214.1. NM_146102.2.
UniGeneiMm.226284.

Genome annotation databases

EnsembliENSMUST00000111584; ENSMUSP00000107210; ENSMUSG00000025083. [Q5DTU0-2]
ENSMUST00000118800; ENSMUSP00000113745; ENSMUSG00000025083. [Q5DTU0-1]
GeneIDi226250.
KEGGimmu:226250.
UCSCiuc008hzm.2. mouse. [Q5DTU0-2]
uc008hzn.2. mouse. [Q5DTU0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK087449 mRNA. Translation: BAC39879.1. Frameshift.
AK220430 mRNA. Translation: BAD90264.1. Different initiation.
BC031515 mRNA. Translation: AAH31515.1. Different initiation.
CCDSiCCDS50476.1. [Q5DTU0-1]
CCDS50477.1. [Q5DTU0-2]
RefSeqiNP_001171267.1. NM_001177796.1. [Q5DTU0-2]
NP_001171268.1. NM_001177797.1. [Q5DTU0-1]
NP_666214.1. NM_146102.2.
UniGeneiMm.226284.

3D structure databases

ProteinModelPortaliQ5DTU0.
SMRiQ5DTU0. Positions 354-443.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ5DTU0. 1 interaction.
MINTiMINT-4117315.
STRINGi10090.ENSMUSP00000107210.

PTM databases

iPTMnetiQ5DTU0.
PhosphoSiteiQ5DTU0.

Proteomic databases

MaxQBiQ5DTU0.
PaxDbiQ5DTU0.
PRIDEiQ5DTU0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000111584; ENSMUSP00000107210; ENSMUSG00000025083. [Q5DTU0-2]
ENSMUST00000118800; ENSMUSP00000113745; ENSMUSG00000025083. [Q5DTU0-1]
GeneIDi226250.
KEGGimmu:226250.
UCSCiuc008hzm.2. mouse. [Q5DTU0-2]
uc008hzn.2. mouse. [Q5DTU0-1]

Organism-specific databases

CTDi84632.
MGIiMGI:2147658. Afap1l2.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IEU6. Eukaryota.
ENOG4111CMN. LUCA.
GeneTreeiENSGT00390000003998.
HOGENOMiHOG000033832.
HOVERGENiHBG106875.
InParanoidiQ5DTU0.
OMAiQWKSRWC.
OrthoDBiEOG7KQ217.
PhylomeDBiQ5DTU0.
TreeFamiTF332622.

Miscellaneous databases

NextBioi378068.
PROiQ5DTU0.
SOURCEiSearch...

Gene expression databases

BgeeiQ5DTU0.
CleanExiMM_AFAP1L2.
ExpressionAtlasiQ5DTU0. baseline and differential.
GenevisibleiQ5DTU0. MM.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR030113. AFAP.
IPR030115. AFAP1L2.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR14338. PTHR14338. 1 hit.
PTHR14338:SF4. PTHR14338:SF4. 1 hit.
PfamiPF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS50003. PH_DOMAIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Eye.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Fetal brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: FVB/N.
    Tissue: Mammary gland.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-56 AND TYR-413, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-113; SER-408 AND SER-484, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Lung, Pancreas and Spleen.

Entry informationi

Entry nameiAF1L2_MOUSE
AccessioniPrimary (citable) accession number: Q5DTU0
Secondary accession number(s): Q8BID1, Q8K2G0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 21, 2005
Last modified: January 20, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.