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Protein

PH and SEC7 domain-containing protein 1

Gene

Psd

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor for ARF6. Isoform 2 and isoform 3 induce cytoskeletal remodeling, but lead to distinct morphological changes in HeLa cells: isoform 2 induces cell elongation and formation of actin-rich protrusions, whereas isoform 3 promotes the formation of membrane ruffles and loss of stress fibers.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
PH and SEC7 domain-containing protein 1
Alternative name(s):
Exchange factor for ARF6
Pleckstrin homology and SEC7 domain-containing protein 1
Gene namesi
Name:Psd
Synonyms:Efa6a, Kiaa2011, Psd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1920978. Psd.

Subcellular locationi

Isoform 1 :
Isoform 2 :
Isoform 3 :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi621 – 6211E → K: Significantly impaired guanine nucleotide exchange factor activity on ARF6 in PC12 cells. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10241024PH and SEC7 domain-containing protein 1PRO_0000334161Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei126 – 1261PhosphoserineCombined sources
Modified residuei156 – 1561PhosphoserineCombined sources
Modified residuei720 – 7201PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ5DTT2.
PaxDbiQ5DTT2.
PeptideAtlasiQ5DTT2.
PRIDEiQ5DTT2.

PTM databases

iPTMnetiQ5DTT2.
PhosphoSiteiQ5DTT2.

Expressioni

Tissue specificityi

Isoform 1 is highly expressed in the strata oriens, radiatum, lacunosum-moleculare of the hippocampal CA1-3 regions and the dentate molecular layer of the hippocampal formation, with lower levels detected in the neuronal cell layers and the stratum lucidum (at protein level). Highest expression detected in brain and some expression detected also in uterus, stomach, ovary and intestine, with isoform 2 being expressed at the highest levels. Not detected in tongue, thymus, spleen, lung, heart, liver and kidney.2 Publications

Developmental stagei

Expressed in embryonic, early postnatal and adult brain, with expression up-regulated at postnatal day 4-8 and down-regulated in adults. Isoform 2 expression is up-regulated in adults.1 Publication

Gene expression databases

BgeeiENSMUSG00000037126.
GenevisibleiQ5DTT2. MM.

Interactioni

Subunit structurei

Interacts with ACTN1 (PubMed:17298598). Interacts (ARF6-bound form) with KCNK1; does not interact with KCNK1 in the absence of ARF6 (PubMed:15540117).2 Publications

Protein-protein interaction databases

BioGridi216216. 1 interaction.
STRINGi10090.ENSMUSP00000039728.

Structurei

3D structure databases

ProteinModelPortaliQ5DTT2.
SMRiQ5DTT2. Positions 559-709.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini512 – 706195SEC7PROSITE-ProRule annotationAdd
BLAST
Domaini756 – 869114PHPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili898 – 92427Sequence analysisAdd
BLAST
Coiled coili956 – 98328Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi18 – 9477Pro-richAdd
BLAST
Compositional biasi432 – 46635Pro-richAdd
BLAST
Compositional biasi739 – 7446Poly-Ser

Sequence similaritiesi

Belongs to the PSD family.Curated
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SEC7 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0932. Eukaryota.
COG5307. LUCA.
GeneTreeiENSGT00760000119036.
HOGENOMiHOG000069986.
HOVERGENiHBG106661.
InParanoidiQ5DTT2.
KOiK12494.
OMAiDAPWTER.
OrthoDBiEOG091G0187.
PhylomeDBiQ5DTT2.
TreeFamiTF319755.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001605. PH_dom-spectrin-type.
IPR001849. PH_domain.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PfamiPF01369. Sec7. 1 hit.
[Graphical view]
PRINTSiPR00683. SPECTRINPH.
SMARTiSM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48425. SSF48425. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative promoter usage and alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5DTT2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQGAMRFCS EGDCAISPPR CPRRWLPEGP VPQSPPASMY GSTGSLIRRV
60 70 80 90 100
VGPGPRGRDL GRVTAPCTPL RAPPSPHIAP SPWGPSSPTG QPPPGAQSSV
110 120 130 140 150
VIFRFVEKAS VRPLNGLPAS GGLSRSWDLG GISAPRPTPA LGPGCNRKLR
160 170 180 190 200
LEASTSDPLP AGGGSVLPGS RDPSRGPLVP PQIGADGLYS SLPNGLGGTP
210 220 230 240 250
EHLAMHFRGP ADTGFLNQGD TWSSPREVSS HAQRIARAKW EFFYGSLDAP
260 270 280 290 300
SSGAKPPEQV LPSRGVGSKQ GSGVAVGRAA KYSETDLDKV PLRCYRETDI
310 320 330 340 350
DEVLAEREEA DSAIESQPSS EGPHGTAQPP ASRPSPCPGP SSSLGSGNED
360 370 380 390 400
DEAGGEEDVD DEVFEASEGA RPGDHMPHSG LLKSPVPFLL GTSPSADGPD
410 420 430 440 450
SFSCVFEAIL ESHRAKGTSY SSLASLEALA SPGPTQSPFF TFEMPPQPPA
460 470 480 490 500
PRPDPPAPAP LAPLEPDSGT SSAADGPWTQ RREVEESDAG ATLAPRKELP
510 520 530 540 550
SPSHSEDSFG LGAAPLGSEP PLSQLVSDSD SELDSTERLA LGSTDTLSNG
560 570 580 590 600
QKADLEAAQR LAKRLYRLDG FRKADVARHL GKNNDFSKLV AGEYLKFFVF
610 620 630 640 650
TGMTLDQALR VFLKELALMG ETQERERVLA HFSQRYFQCN PEALSSEDGA
660 670 680 690 700
HTLTCALMLL NTDLHGHNIG KRMTCGDFIG NLEGLNDGGD FPRELLKALY
710 720 730 740 750
SSIKNEKLQW AIDEEELRRS LSELADPNPK VIKRVSGGSG SSSSPFLDLT
760 770 780 790 800
PEPGAAVYKH GALVRKVHAD PDCRKTPRGK RGWKSFHGIL KGMILYLQKE
810 820 830 840 850
EYQPGKALSE AELKNAISIH HALATRASDY SKRPHVFYLR TADWRVFLFQ
860 870 880 890 900
APSLEQMQSW ITRINVVAAM FSAPPFPAAV SSQKKFSRPL LPSAATRLSQ
910 920 930 940 950
EEQVRTHEAK LKAMASELRE HRAAHLGKKA RGKEADEQRQ KEAYLEFEKS
960 970 980 990 1000
RYGTYAALLR VKMKAASEEL DTIEAALAQA GSTEDGCPPP HSSPSLRPKP
1010 1020
TSQPRAQRPG SETRAGAGST RPKP
Length:1,024
Mass (Da):109,687
Last modified:May 20, 2008 - v2
Checksum:i207A889E6D16CC99
GO
Isoform 2 (identifier: Q5DTT2-2) [UniParc]FASTAAdd to basket
Also known as: EFA6As

The sequence of this isoform differs from the canonical sequence as follows:
     1-631: Missing.
     632-697: FSQRYFQCNP...GGDFPRELLK → MIGVNSIHSS...RLQSRKKKKA

Note: Produced by alternative promoter usage.
Show »
Length:393
Mass (Da):43,715
Checksum:i57B83FA12E6D4035
GO
Isoform 3 (identifier: Q5DTT2-3) [UniParc]FASTAAdd to basket
Also known as: EFA6A

The sequence of this isoform differs from the canonical sequence as follows:
     518-518: S → SS

Note: Produced by alternative splicing of isoform 1.
Show »
Length:1,025
Mass (Da):109,774
Checksum:i5AF3EAC9C00976A4
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 631631Missing in isoform 2. 2 PublicationsVSP_033637Add
BLAST
Alternative sequencei518 – 5181S → SS in isoform 3. 2 PublicationsVSP_041569
Alternative sequencei632 – 69766FSQRY…RELLK → MIGVNSIHSSAGRLRSRSLC SVRYGRTHRGAETLCYGWPQ RSRSLKPVLYTDLVVSRLQS RKKKKA in isoform 2. 2 PublicationsVSP_033638Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC114539 Genomic DNA. No translation available.
AK158851 mRNA. Translation: BAE34694.1.
BC058352 mRNA. Translation: AAH58352.1.
BC138652 mRNA. Translation: AAI38653.1.
BC145352 mRNA. Translation: AAI45353.1.
AK220438 Transcribed RNA. Translation: BAD90268.1.
CCDSiCCDS29875.1. [Q5DTT2-1]
RefSeqiNP_082903.2. NM_028627.2. [Q5DTT2-1]
XP_006527449.1. XM_006527386.2. [Q5DTT2-3]
XP_006527451.1. XM_006527388.2. [Q5DTT2-3]
XP_006527452.1. XM_006527389.2. [Q5DTT2-3]
XP_006527453.1. XM_006527390.2. [Q5DTT2-3]
XP_006527454.1. XM_006527391.2. [Q5DTT2-3]
XP_006527456.1. XM_006527393.2. [Q5DTT2-2]
XP_011245677.1. XM_011247375.1. [Q5DTT2-3]
XP_011245678.1. XM_011247376.1. [Q5DTT2-3]
XP_011245679.1. XM_011247377.1. [Q5DTT2-1]
UniGeneiMm.268532.

Genome annotation databases

EnsembliENSMUST00000041391; ENSMUSP00000039728; ENSMUSG00000037126. [Q5DTT2-1]
ENSMUST00000096029; ENSMUSP00000093729; ENSMUSG00000037126. [Q5DTT2-3]
GeneIDi73728.
KEGGimmu:73728.
UCSCiuc008htb.1. mouse. [Q5DTT2-2]
uc008htd.1. mouse. [Q5DTT2-1]
uc012bmt.1. mouse. [Q5DTT2-3]

Keywords - Coding sequence diversityi

Alternative promoter usage, Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC114539 Genomic DNA. No translation available.
AK158851 mRNA. Translation: BAE34694.1.
BC058352 mRNA. Translation: AAH58352.1.
BC138652 mRNA. Translation: AAI38653.1.
BC145352 mRNA. Translation: AAI45353.1.
AK220438 Transcribed RNA. Translation: BAD90268.1.
CCDSiCCDS29875.1. [Q5DTT2-1]
RefSeqiNP_082903.2. NM_028627.2. [Q5DTT2-1]
XP_006527449.1. XM_006527386.2. [Q5DTT2-3]
XP_006527451.1. XM_006527388.2. [Q5DTT2-3]
XP_006527452.1. XM_006527389.2. [Q5DTT2-3]
XP_006527453.1. XM_006527390.2. [Q5DTT2-3]
XP_006527454.1. XM_006527391.2. [Q5DTT2-3]
XP_006527456.1. XM_006527393.2. [Q5DTT2-2]
XP_011245677.1. XM_011247375.1. [Q5DTT2-3]
XP_011245678.1. XM_011247376.1. [Q5DTT2-3]
XP_011245679.1. XM_011247377.1. [Q5DTT2-1]
UniGeneiMm.268532.

3D structure databases

ProteinModelPortaliQ5DTT2.
SMRiQ5DTT2. Positions 559-709.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi216216. 1 interaction.
STRINGi10090.ENSMUSP00000039728.

PTM databases

iPTMnetiQ5DTT2.
PhosphoSiteiQ5DTT2.

Proteomic databases

MaxQBiQ5DTT2.
PaxDbiQ5DTT2.
PeptideAtlasiQ5DTT2.
PRIDEiQ5DTT2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041391; ENSMUSP00000039728; ENSMUSG00000037126. [Q5DTT2-1]
ENSMUST00000096029; ENSMUSP00000093729; ENSMUSG00000037126. [Q5DTT2-3]
GeneIDi73728.
KEGGimmu:73728.
UCSCiuc008htb.1. mouse. [Q5DTT2-2]
uc008htd.1. mouse. [Q5DTT2-1]
uc012bmt.1. mouse. [Q5DTT2-3]

Organism-specific databases

CTDi5662.
MGIiMGI:1920978. Psd.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0932. Eukaryota.
COG5307. LUCA.
GeneTreeiENSGT00760000119036.
HOGENOMiHOG000069986.
HOVERGENiHBG106661.
InParanoidiQ5DTT2.
KOiK12494.
OMAiDAPWTER.
OrthoDBiEOG091G0187.
PhylomeDBiQ5DTT2.
TreeFamiTF319755.

Miscellaneous databases

PROiQ5DTT2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037126.
GenevisibleiQ5DTT2. MM.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001605. PH_dom-spectrin-type.
IPR001849. PH_domain.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PfamiPF01369. Sec7. 1 hit.
[Graphical view]
PRINTSiPR00683. SPECTRINPH.
SMARTiSM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48425. SSF48425. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSD1_MOUSE
AccessioniPrimary (citable) accession number: Q5DTT2
Secondary accession number(s): B2RS07
, B7ZNN7, Q3TY69, Q6PE10
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: September 7, 2016
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.