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Protein

E3 ubiquitin-protein ligase BRE1A

Gene

Rnf20

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of PA2G4 leading to its proteasome-mediated degradation.By similarity

Caution

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri920 – 959RING-typePROSITE-ProRule annotationAdd BLAST40

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Transferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-8866654 E3 ubiquitin ligases ubiquitinate target proteins
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase BRE1A (EC:2.3.2.27By similarity)
Short name:
BRE1-A
Alternative name(s):
RING finger protein 20
RING-type E3 ubiquitin transferase BRE1ACurated
Gene namesi
Name:Rnf20
Synonyms:Bre1a, Kiaa4116
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1925927 Rnf20

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000558371 – 973E3 ubiquitin-protein ligase BRE1AAdd BLAST973

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei21N6-acetyllysineBy similarity1
Modified residuei41PhosphoserineBy similarity1
Modified residuei136PhosphoserineCombined sources1
Modified residuei138PhosphoserineCombined sources1
Modified residuei348N6-acetyllysineBy similarity1
Modified residuei510N6-acetyllysineBy similarity1
Modified residuei522PhosphoserineBy similarity1
Modified residuei560PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ5DTM8
PaxDbiQ5DTM8
PeptideAtlasiQ5DTM8
PRIDEiQ5DTM8

PTM databases

iPTMnetiQ5DTM8
PhosphoSitePlusiQ5DTM8

Expressioni

Gene expression databases

BgeeiENSMUSG00000028309
CleanExiMM_RNF20
ExpressionAtlasiQ5DTM8 baseline and differential
GenevisibleiQ5DTM8 MM

Interactioni

Subunit structurei

Component of the RNF20/40 complex (also known as BRE1 complex) probably composed of 2 copies of RNF20/BRE1A and 2 copies of RNF40/BRE1B. Interacts with UBE2E1/UBCH6. Interacts with p53/TP53 and WAC. Interacts with PAF1; the interaction mediates the association of the PAF1 and RNF20/40 complexes which is a prerequsite for recruitment of UBE2A/B. Interacts with PA2G4.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi2246495 interactors.
IntActiQ5DTM8 2 interactors.
MINTiQ5DTM8
STRINGi10090.ENSMUSP00000029989

Structurei

3D structure databases

ProteinModelPortaliQ5DTM8
SMRiQ5DTM8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili43 – 90Sequence analysisAdd BLAST48
Coiled coili168 – 378Sequence analysisAdd BLAST211
Coiled coili429 – 896Sequence analysisAdd BLAST468

Sequence similaritiesi

Belongs to the BRE1 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri920 – 959RING-typePROSITE-ProRule annotationAdd BLAST40

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG0978 Eukaryota
ENOG410Y5C6 LUCA
GeneTreeiENSGT00390000002866
HOVERGENiHBG080312
InParanoidiQ5DTM8
KOiK10696
OMAiNRYWNQF
OrthoDBiEOG091G01SA
PhylomeDBiQ5DTM8
TreeFamiTF323183

Family and domain databases

Gene3Di3.30.40.101 hit
InterProiView protein in InterPro
IPR013956 E3_ubiquit_lig_Bre1
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR23163 PTHR23163, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5DTM8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGIGNKRAA GEPGTSMPPE KKTAVEDSGT TVETIKLGGV SSTEELDIRT
60 70 80 90 100
LQSKNRKLAE MLDQRQAIED ELREHIEKLE RRQATDDASL LIVNRYWSQF
110 120 130 140 150
DENIRIILKR YDLDQGLGDL LTERKALVVP EPEPDSDSNQ ERKDDRERGD
160 170 180 190 200
GQEPAFSFLA TLASSSSEEM ESQLQERVES SRRAVSQIVT VYDKLQEKVD
210 220 230 240 250
LLSRKLNSGD NLIVEEAVQE LNSFLAQENV RLQELTDLLQ EKHHTMSQEF
260 270 280 290 300
CKLQGKVETA ESRVSVLESM IDDLQWDIDK IRKREQRLNR HLAEVLERVN
310 320 330 340 350
SKGYKVYGAG SSLYGGTITI NARKFEEMNA ELEENKELAQ NRHCELEKLR
360 370 380 390 400
QDFEEVTTQN EKLKVELRSA VEEVVKETPE YRCMQSQFSV LYNESLQLKA
410 420 430 440 450
HLDEARTLLH GTRGTHQRQV ELIERDEVSL HKKLRTEVIQ LEDTLAQVRK
460 470 480 490 500
EYEMLRIEFE QTLAANEQAG PINREMRHLI SSLQNHNHQL KGEVLRYKRK
510 520 530 540 550
LREAQSDLNK TRLRSGSALL QSQSSTEDPK DEPTELKQDS EDLATHSSAL
560 570 580 590 600
KASQEDEVKS KRDEEERERE RREKERERER EREKEKERER EKQKLKESEK
610 620 630 640 650
ERDSVKDKEK GKHDDGRKKE AEIIKQLKIE LKKAQESQKE MKLLLDMYRS
660 670 680 690 700
APKEQRDKVQ LMAAEKKSKA ELEDLRQRLK DLEDKEKKEN KKMADEDALR
710 720 730 740 750
KIRAVEEQIE YLQKKLAMAK QEEEALLSEM DVTGQAFEDM QEQNIRLMQQ
760 770 780 790 800
LREKDDANFK LMSERIKSNQ IHKLLKEEKE ELADQVLTLK TQVDAQLQVV
810 820 830 840 850
RKLEEKEHLL QSNIGTGEKE LGLRTQALEM NKRKAMEAAQ LADDLKAQLE
860 870 880 890 900
LAQKKLHDFQ DEIVENSVTK EKDLFNFKRA QEDISRLRRK LETTKKPDNV
910 920 930 940 950
PKCDEILMEE IKDYKARLTC PCCNMRKKDA VLTKCFHVFC FECVKTRYDT
960 970
RQRKCPKCNA AFGANDFHRI YIG
Length:973
Mass (Da):113,520
Last modified:December 20, 2005 - v2
Checksum:i6A89582C7ECD556C
GO
Isoform 2 (identifier: Q5DTM8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     100-249: Missing.

Note: No experimental confirmation available.
Show »
Length:823
Mass (Da):96,349
Checksum:i2163D9DFF8F048AC
GO

Sequence cautioni

The sequence BAD90290 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti199V → L in BAC36367 (PubMed:16141072).Curated1
Sequence conflicti506S → F in BAC36367 (PubMed:16141072).Curated1
Sequence conflicti540S → F in BAC36367 (PubMed:16141072).Curated1
Sequence conflicti607D → G in BAE43337 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016679100 – 249Missing in isoform 2. 1 PublicationAdd BLAST150

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220492 mRNA Translation: BAD90290.1 Different initiation.
AL732521 Genomic DNA Translation: CAM14732.1
BC052482 mRNA Translation: AAH52482.1
AK048862 mRNA Translation: BAE43337.1
AK051278 mRNA Translation: BAC34590.2
AK053783 mRNA Translation: BAC35521.1
AK076501 mRNA Translation: BAC36367.1
AK085436 mRNA Translation: BAC39446.1
AK139883 mRNA Translation: BAE24170.1
CCDSiCCDS18178.1 [Q5DTM8-1]
RefSeqiNP_001156735.1, NM_001163263.1 [Q5DTM8-1]
NP_892044.1, NM_182999.2 [Q5DTM8-1]
XP_006537616.1, XM_006537553.3 [Q5DTM8-1]
XP_011248208.1, XM_011249906.2 [Q5DTM8-1]
UniGeneiMm.24765

Genome annotation databases

EnsembliENSMUST00000029989; ENSMUSP00000029989; ENSMUSG00000028309 [Q5DTM8-1]
ENSMUST00000167496; ENSMUSP00000128546; ENSMUSG00000028309 [Q5DTM8-1]
GeneIDi109331
KEGGimmu:109331
UCSCiuc008swa.2 mouse [Q5DTM8-1]
uc012dec.1 mouse [Q5DTM8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiBRE1A_MOUSE
AccessioniPrimary (citable) accession number: Q5DTM8
Secondary accession number(s): A2AIR3
, Q3UT10, Q3V350, Q7TT11, Q8BKA8, Q8BKN8, Q8BUF7, Q8BVU4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: February 28, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome