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Protein

E3 ubiquitin-protein ligase BRE1A

Gene

Rnf20

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of PA2G4 leading to its proteasome-mediated degradation.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri920 – 95940RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase BRE1A (EC:6.3.2.-)
Short name:
BRE1-A
Alternative name(s):
RING finger protein 20
Gene namesi
Name:Rnf20
Synonyms:Bre1a, Kiaa4116
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1925927. Rnf20.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 973973E3 ubiquitin-protein ligase BRE1APRO_0000055837Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei21 – 211N6-acetyllysineBy similarity
Modified residuei136 – 1361PhosphoserineCombined sources
Modified residuei138 – 1381PhosphoserineCombined sources
Modified residuei348 – 3481N6-acetyllysineBy similarity
Modified residuei510 – 5101N6-acetyllysineBy similarity
Modified residuei522 – 5221PhosphoserineBy similarity
Modified residuei560 – 5601PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ5DTM8.
MaxQBiQ5DTM8.
PaxDbiQ5DTM8.
PRIDEiQ5DTM8.

PTM databases

iPTMnetiQ5DTM8.
PhosphoSiteiQ5DTM8.

Expressioni

Gene expression databases

BgeeiQ5DTM8.
CleanExiMM_RNF20.
ExpressionAtlasiQ5DTM8. baseline and differential.
GenevisibleiQ5DTM8. MM.

Interactioni

Subunit structurei

Component of the RNF20/40 complex (also known as BRE1 complex) probably composed of 2 copies of RNF20/BRE1A and 2 copies of RNF40/BRE1B. Interacts with UBE2E1/UBCH6. Interacts with p53/TP53 and WAC. Interacts with PAF1; the interaction mediates the association of the PAF1 and RNF20/40 complexes which is a prerequsite for recruitment of UBE2A/B. Interacts with PA2G4.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi224649. 4 interactions.
IntActiQ5DTM8. 2 interactions.
MINTiMINT-4112835.
STRINGi10090.ENSMUSP00000029989.

Structurei

3D structure databases

ProteinModelPortaliQ5DTM8.
SMRiQ5DTM8. Positions 904-972.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili43 – 9048Sequence analysisAdd
BLAST
Coiled coili168 – 378211Sequence analysisAdd
BLAST
Coiled coili429 – 896468Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the BRE1 family.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri920 – 95940RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG0978. Eukaryota.
ENOG410Y5C6. LUCA.
GeneTreeiENSGT00390000002866.
HOVERGENiHBG080312.
InParanoidiQ5DTM8.
KOiK10696.
OMAiNKELAGN.
PhylomeDBiQ5DTM8.
TreeFamiTF323183.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5DTM8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGIGNKRAA GEPGTSMPPE KKTAVEDSGT TVETIKLGGV SSTEELDIRT
60 70 80 90 100
LQSKNRKLAE MLDQRQAIED ELREHIEKLE RRQATDDASL LIVNRYWSQF
110 120 130 140 150
DENIRIILKR YDLDQGLGDL LTERKALVVP EPEPDSDSNQ ERKDDRERGD
160 170 180 190 200
GQEPAFSFLA TLASSSSEEM ESQLQERVES SRRAVSQIVT VYDKLQEKVD
210 220 230 240 250
LLSRKLNSGD NLIVEEAVQE LNSFLAQENV RLQELTDLLQ EKHHTMSQEF
260 270 280 290 300
CKLQGKVETA ESRVSVLESM IDDLQWDIDK IRKREQRLNR HLAEVLERVN
310 320 330 340 350
SKGYKVYGAG SSLYGGTITI NARKFEEMNA ELEENKELAQ NRHCELEKLR
360 370 380 390 400
QDFEEVTTQN EKLKVELRSA VEEVVKETPE YRCMQSQFSV LYNESLQLKA
410 420 430 440 450
HLDEARTLLH GTRGTHQRQV ELIERDEVSL HKKLRTEVIQ LEDTLAQVRK
460 470 480 490 500
EYEMLRIEFE QTLAANEQAG PINREMRHLI SSLQNHNHQL KGEVLRYKRK
510 520 530 540 550
LREAQSDLNK TRLRSGSALL QSQSSTEDPK DEPTELKQDS EDLATHSSAL
560 570 580 590 600
KASQEDEVKS KRDEEERERE RREKERERER EREKEKERER EKQKLKESEK
610 620 630 640 650
ERDSVKDKEK GKHDDGRKKE AEIIKQLKIE LKKAQESQKE MKLLLDMYRS
660 670 680 690 700
APKEQRDKVQ LMAAEKKSKA ELEDLRQRLK DLEDKEKKEN KKMADEDALR
710 720 730 740 750
KIRAVEEQIE YLQKKLAMAK QEEEALLSEM DVTGQAFEDM QEQNIRLMQQ
760 770 780 790 800
LREKDDANFK LMSERIKSNQ IHKLLKEEKE ELADQVLTLK TQVDAQLQVV
810 820 830 840 850
RKLEEKEHLL QSNIGTGEKE LGLRTQALEM NKRKAMEAAQ LADDLKAQLE
860 870 880 890 900
LAQKKLHDFQ DEIVENSVTK EKDLFNFKRA QEDISRLRRK LETTKKPDNV
910 920 930 940 950
PKCDEILMEE IKDYKARLTC PCCNMRKKDA VLTKCFHVFC FECVKTRYDT
960 970
RQRKCPKCNA AFGANDFHRI YIG
Length:973
Mass (Da):113,520
Last modified:December 20, 2005 - v2
Checksum:i6A89582C7ECD556C
GO
Isoform 2 (identifier: Q5DTM8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     100-249: Missing.

Note: No experimental confirmation available.
Show »
Length:823
Mass (Da):96,349
Checksum:i2163D9DFF8F048AC
GO

Sequence cautioni

The sequence BAD90290.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti199 – 1991V → L in BAC36367 (PubMed:16141072).Curated
Sequence conflicti506 – 5061S → F in BAC36367 (PubMed:16141072).Curated
Sequence conflicti540 – 5401S → F in BAC36367 (PubMed:16141072).Curated
Sequence conflicti607 – 6071D → G in BAE43337 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei100 – 249150Missing in isoform 2. 1 PublicationVSP_016679Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220492 mRNA. Translation: BAD90290.1. Different initiation.
AL732521 Genomic DNA. Translation: CAM14732.1.
BC052482 mRNA. Translation: AAH52482.1.
AK048862 mRNA. Translation: BAE43337.1.
AK051278 mRNA. Translation: BAC34590.2.
AK053783 mRNA. Translation: BAC35521.1.
AK076501 mRNA. Translation: BAC36367.1.
AK085436 mRNA. Translation: BAC39446.1.
AK139883 mRNA. Translation: BAE24170.1.
CCDSiCCDS18178.1. [Q5DTM8-1]
RefSeqiNP_001156735.1. NM_001163263.1. [Q5DTM8-1]
NP_892044.1. NM_182999.2. [Q5DTM8-1]
XP_006537616.1. XM_006537553.2. [Q5DTM8-1]
XP_011248208.1. XM_011249906.1. [Q5DTM8-1]
UniGeneiMm.24765.

Genome annotation databases

EnsembliENSMUST00000029989; ENSMUSP00000029989; ENSMUSG00000028309. [Q5DTM8-1]
ENSMUST00000167496; ENSMUSP00000128546; ENSMUSG00000028309. [Q5DTM8-1]
GeneIDi109331.
KEGGimmu:109331.
UCSCiuc008swa.2. mouse. [Q5DTM8-1]
uc012dec.1. mouse. [Q5DTM8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220492 mRNA. Translation: BAD90290.1. Different initiation.
AL732521 Genomic DNA. Translation: CAM14732.1.
BC052482 mRNA. Translation: AAH52482.1.
AK048862 mRNA. Translation: BAE43337.1.
AK051278 mRNA. Translation: BAC34590.2.
AK053783 mRNA. Translation: BAC35521.1.
AK076501 mRNA. Translation: BAC36367.1.
AK085436 mRNA. Translation: BAC39446.1.
AK139883 mRNA. Translation: BAE24170.1.
CCDSiCCDS18178.1. [Q5DTM8-1]
RefSeqiNP_001156735.1. NM_001163263.1. [Q5DTM8-1]
NP_892044.1. NM_182999.2. [Q5DTM8-1]
XP_006537616.1. XM_006537553.2. [Q5DTM8-1]
XP_011248208.1. XM_011249906.1. [Q5DTM8-1]
UniGeneiMm.24765.

3D structure databases

ProteinModelPortaliQ5DTM8.
SMRiQ5DTM8. Positions 904-972.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi224649. 4 interactions.
IntActiQ5DTM8. 2 interactions.
MINTiMINT-4112835.
STRINGi10090.ENSMUSP00000029989.

PTM databases

iPTMnetiQ5DTM8.
PhosphoSiteiQ5DTM8.

Proteomic databases

EPDiQ5DTM8.
MaxQBiQ5DTM8.
PaxDbiQ5DTM8.
PRIDEiQ5DTM8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029989; ENSMUSP00000029989; ENSMUSG00000028309. [Q5DTM8-1]
ENSMUST00000167496; ENSMUSP00000128546; ENSMUSG00000028309. [Q5DTM8-1]
GeneIDi109331.
KEGGimmu:109331.
UCSCiuc008swa.2. mouse. [Q5DTM8-1]
uc012dec.1. mouse. [Q5DTM8-2]

Organism-specific databases

CTDi56254.
MGIiMGI:1925927. Rnf20.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0978. Eukaryota.
ENOG410Y5C6. LUCA.
GeneTreeiENSGT00390000002866.
HOVERGENiHBG080312.
InParanoidiQ5DTM8.
KOiK10696.
OMAiNKELAGN.
PhylomeDBiQ5DTM8.
TreeFamiTF323183.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

ChiTaRSiRnf20. mouse.
NextBioi361921.
PROiQ5DTM8.
SOURCEiSearch...

Gene expression databases

BgeeiQ5DTM8.
CleanExiMM_RNF20.
ExpressionAtlasiQ5DTM8. baseline and differential.
GenevisibleiQ5DTM8. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Fetal brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Olfactory epithelium.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-607 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-616 (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Cerebellum, Egg, Eye, Head, Kidney and Spinal ganglion.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-136 AND SER-138, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiBRE1A_MOUSE
AccessioniPrimary (citable) accession number: Q5DTM8
Secondary accession number(s): A2AIR3
, Q3UT10, Q3V350, Q7TT11, Q8BKA8, Q8BKN8, Q8BUF7, Q8BVU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 16, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.