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Q5DTK1 (CHSS3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chondroitin sulfate synthase 3
EC=2.4.1.175
EC=2.4.1.226
Alternative name(s):
Carbohydrate synthase 2
Chondroitin glucuronyltransferase 3
Chondroitin synthase 2
Short name=ChSy-2
Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II
N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 3
N-acetylgalactosaminyltransferase 3
Gene names
Name:Chsy3
Synonyms:Chsy2, Css3, Kiaa4168
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length884 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Specific activity is much reduced compared to CHSY1 By similarity.

Catalytic activity

UDP-N-acetyl-D-galactosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan = UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan.

UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.

Cofactor

Divalent cations. Highest activities are measured with cobalt, manganese and cadmium By similarity.

Subcellular location

Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein Probable.

Sequence similarities

Belongs to the chondroitin N-acetylgalactosaminyltransferase family.

Sequence caution

The sequence BAD90307.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 884884Chondroitin sulfate synthase 3
PRO_0000320145

Regions

Topological domain1 – 77Cytoplasmic Potential
Transmembrane8 – 2821Helical; Signal-anchor for type II membrane protein; Potential
Topological domain29 – 884856Lumenal Potential
Compositional bias211 – 23727Pro-rich
Compositional bias541 – 5466Poly-Leu

Sites

Metal binding7221Divalent metal cation Potential
Metal binding8361Divalent metal cation Potential

Amino acid modifications

Modified residue4131Phosphoserine By similarity
Modified residue4721Phosphothreonine Ref.4
Glycosylation1551N-linked (GlcNAc...) Potential
Glycosylation2811N-linked (GlcNAc...) Potential
Glycosylation7121N-linked (GlcNAc...) Potential
Glycosylation8801N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict1081L → P in BAD90307. Ref.1
Sequence conflict1641V → A in BAD90307. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q5DTK1 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: D1D9E9EAA6D2FB3E

FASTA884100,099
        10         20         30         40         50         60 
MAVRSRRPWV SVALGLVLGF TAASWLIAPR VAELSEKRRR GSSLCSYYGR SATGPRADAQ 

        70         80         90        100        110        120 
QLLPQPQSRP RLEQSPPPAS HELPGPQQPE AAPGGPSFRS SPWQQPALLP QRRRGHTPEG 

       130        140        150        160        170        180 
ATALPGAPAA KGEPEEEDGG AADPRKGGRP GSSHNGSGDG GAAVPTSGPG DFLYVGVMTA 

       190        200        210        220        230        240 
QKYLGSRALA AQRTWARFIP GRVEFFSSQQ SPSAALGQPP PPLPVIALPG VDDSYPPQKK 

       250        260        270        280        290        300 
SFMMIKYMHD HYLDKYEWFM RADDDVYIKG DKLEEFLRSL NSSKPLYLGQ TGLGNTEELG 

       310        320        330        340        350        360 
KLGLEPGENF CMGGPGMIFS REVLRRMVPH IGECLREMYT THEDVEVGRC VRRFGGTQCV 

       370        380        390        400        410        420 
WSYEMQQLFH ENYEHNRKGY IQDLHNSKIH AAITLHPNKR PAYQYRLHNY MLSRKISELR 

       430        440        450        460        470        480 
YRTIQLHRES ALMSKLSNSE VSKEDQQLGR TPSFNHFQPR ERNEVMEWEF LTGKLLYSAA 

       490        500        510        520        530        540 
ENQPPRQSIN SILRSALDDT VLQVMEMINE NAKSRGRLID FKEIQYGYRR VDPMHGVEYI 

       550        560        570        580        590        600 
LDLLLLYKRH KGRKLTVPVR RHAYLQQPFS KPFFREVEEL DVNRLVESIN SGTQSFSVIS 

       610        620        630        640        650        660 
NSLKILSSLQ EAKDIGGHNE KKVHILVPLV GRYDIFLRFM ENFESTCLIP KQNVKLVIIL 

       670        680        690        700        710        720 
FSRDAGQESI KHIELIQEYQ SRYPSAEMML IPMKGEFSRG LGLEMASSQF DNDTLLLFCD 

       730        740        750        760        770        780 
VDLIFRGDFL QRCRDNTVQG QQVYYPIIFS QYDPKVTHMR NPPTEGDFVF SKETGFWRDY 

       790        800        810        820        830        840 
GYGITCIYKS DLLGAGGFDT SIQGWGLEDV DLYNKVILSG LRPFRSQEVG VVHIFHPVHC 

       850        860        870        880 
DPNLDPKQYK MCLGSKASTF ASTMQLAELW LEKHLGVRDN RTLS

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References

[1]"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Fetal brain.
[2]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry."
Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R.
J. Proteome Res. 6:250-262(2007) [PubMed: 17203969] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-472, MASS SPECTROMETRY.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AK220519 mRNA. Translation: BAD90307.1. Different initiation.
CH466528 Genomic DNA. Translation: EDL09842.1.
BC151164 mRNA. Translation: AAI51165.1.
BC151165 mRNA. Translation: AAI51166.1.
BC157985 mRNA. Translation: AAI57986.1.
IPIIPI00874709.
RefSeqNP_001074797.1. NM_001081328.1.
UniGeneMm.485185.
Mm.84007.

3D structure databases

ProteinModelPortalQ5DTK1.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ5DTK1.

Protein family/group databases

CAZyGT31. Glycosyltransferase Family 31.
GT7. Glycosyltransferase Family 7.

PTM databases

PhosphoSiteQ5DTK1.

Proteomic databases

PRIDEQ5DTK1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000080721; ENSMUSP00000079546; ENSMUSG00000058152.
GeneID78923.
KEGGmmu:78923.

Organism-specific databases

CTD337876.
MGIMGI:1926173. Chsy3.
RougeSearch...

Phylogenomic databases

HOGENOMHBG379060.
HOVERGENHBG050948.
InParanoidQ5DTK1.
OrthoDBEOG43XV2V.

Gene expression databases

ArrayExpressQ5DTK1.
BgeeQ5DTK1.
CleanExMM_CHSY3.
GenevestigatorQ5DTK1.

Family and domain databases

InterProIPR008428. Chond_GalNAc.
[Graphical view]
KOK13499.
PANTHERPTHR12369. CHGN. 1 hit.
PfamPF05679. CHGN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio349766.
SOURCESearch...

Entry information

Entry nameCHSS3_MOUSE
AccessionPrimary (citable) accession number: Q5DTK1
Secondary accession number(s): B2RXU7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: July 27, 2011
Last modified: December 14, 2011
This is version 53 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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