Reviewed, UniProtKB/Swiss-Prot Q5DTK1 (CHSS3_MOUSE)

Names and origin

Protein names

Recommended name:
    Chondroitin sulfate synthase 3
    EC=2.4.1.175
    EC=2.4.1.226
Alternative name(s):
    Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II
    Chondroitin synthase 2
      Short name=ChSy-2
    N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 3
    Chondroitin glucuronyltransferase 3
    N-acetylgalactosaminyltransferase 3
    Carbohydrate synthase 2

Gene names

Name:	Chsy3
Synonyms:	Chsy2, Css3, Kiaa4168

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	884 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Specific activity is much reduced compared to CHSY1 By similarity.
Catalytic activity	UDP-N-acetyl-D-galactosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan = UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan. UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.
Cofactor	Divalent cations. Highest activities are measured with cobalt, manganese and cadmium By similarity.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein Probable.
Sequence similarities	Belongs to the chondroitin N-acetylgalactosaminyltransferase family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Metal-binding
Molecular function	Transferase
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase activity Inferred from electronic annotation. Source: EC glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 884	884	Chondroitin sulfate synthase 3		PRO_0000320145
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 28	21	Signal-anchor for type II membrane protein Potential
	Topological domain	29 – 884	856	Lumenal Potential
	Compositional bias	211 – 237	27	Pro-rich
	Compositional bias	541 – 546	6	Poly-Leu
Sites
	Metal binding	722	1	Divalent metal cation Potential
	Metal binding	836	1	Divalent metal cation Potential
Amino acid modifications
	Modified residue	413	1	Phosphoserine By similarity
	Modified residue	472	1	Phosphothreonine Ref.2
	Glycosylation	155	1	N-linked (GlcNAc...) Potential
	Glycosylation	281	1	N-linked (GlcNAc...) Potential
	Glycosylation	712	1	N-linked (GlcNAc...) Potential
	Glycosylation	880	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5DTK1-1 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: 4F7CB2DB9BCF8C1F	FASTA	884	100,055
	10 20 30 40 50 60 MAVRSRRPWV SVALGLVLGF TAASWLIAPR VAELSEKRRR GSSLCSYYGR SATGPRADAQ 70 80 90 100 110 120 QLLPQPQSRP RLEQSPPPAS HELPGPQQPE AAPGGPSFRS SPWQQPAPLP QRRRGHTPEG 130 140 150 160 170 180 ATALPGAPAA KGEPEEEDGG AADPRKGGRP GSSHNGSGDG GAAAPTSGPG DFLYVGVMTA 190 200 210 220 230 240 QKYLGSRALA AQRTWARFIP GRVEFFSSQQ SPSAALGQPP PPLPVIALPG VDDSYPPQKK 250 260 270 280 290 300 SFMMIKYMHD HYLDKYEWFM RADDDVYIKG DKLEEFLRSL NSSKPLYLGQ TGLGNTEELG 310 320 330 340 350 360 KLGLEPGENF CMGGPGMIFS REVLRRMVPH IGECLREMYT THEDVEVGRC VRRFGGTQCV 370 380 390 400 410 420 WSYEMQQLFH ENYEHNRKGY IQDLHNSKIH AAITLHPNKR PAYQYRLHNY MLSRKISELR 430 440 450 460 470 480 YRTIQLHRES ALMSKLSNSE VSKEDQQLGR TPSFNHFQPR ERNEVMEWEF LTGKLLYSAA 490 500 510 520 530 540 ENQPPRQSIN SILRSALDDT VLQVMEMINE NAKSRGRLID FKEIQYGYRR VDPMHGVEYI 550 560 570 580 590 600 LDLLLLYKRH KGRKLTVPVR RHAYLQQPFS KPFFREVEEL DVNRLVESIN SGTQSFSVIS 610 620 630 640 650 660 NSLKILSSLQ EAKDIGGHNE KKVHILVPLV GRYDIFLRFM ENFESTCLIP KQNVKLVIIL 670 680 690 700 710 720 FSRDAGQESI KHIELIQEYQ SRYPSAEMML IPMKGEFSRG LGLEMASSQF DNDTLLLFCD 730 740 750 760 770 780 VDLIFRGDFL QRCRDNTVQG QQVYYPIIFS QYDPKVTHMR NPPTEGDFVF SKETGFWRDY 790 800 810 820 830 840 GYGITCIYKS DLLGAGGFDT SIQGWGLEDV DLYNKVILSG LRPFRSQEVG VVHIFHPVHC 850 860 870 880 DPNLDPKQYK MCLGSKASTF ASTMQLAELW LEKHLGVRDN RTLS

References

[1]	"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H. Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Fetal brain.
[2]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed: 17203969] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-472, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK220519 mRNA. Translation: BAD90307.1. Different initiation.
IPI	IPI00874709.
UniGene	Mm.84007
3D structure databases
SMR	Q5DTK1. Positions 172-396, 615-835.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5DTK1.
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31. GT7. Glycosyltransferase Family 7.
PTM databases
PhosphoSite	Q5DTK1.
Proteomic databases
PRIDE	Q5DTK1.
Genome annotation databases
Ensembl	ENSMUST00000080721; ENSMUSP00000079546; ENSMUSG00000058152; Mus musculus. [Genome view]
UCSC	uc008ezw.1. mouse.
Organism-specific databases
MGI	MGI:1926173. Chsy3.
Rouge	Search...
Phylogenomic databases
HOGENOM	HBG379060.
HOVERGEN	Q5DTK1.
InParanoid	Q5DTK1.
Enzyme and pathway databases
BRENDA	2.4.1.175. 244. 2.4.1.226. 244.
Gene expression databases
ArrayExpress	Q5DTK1.
Bgee	Q5DTK1.
CleanEx	MM_CHSY3.
Genevestigator	Q5DTK1.
Family and domain databases
InterPro	IPR008428. Chond_GalNAc. [Graphical view]
PANTHER	PTHR12369. CHGN. 1 hit.
Pfam	PF05679. CHGN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

CHSS3_MOUSE

Accession

Primary (citable) accession number: Q5DTK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	February 26, 2008
Last modified:	February 9, 2010
This is version 37 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)