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Protein

Chondroitin sulfate synthase 3

Gene

Chsy3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Specific activity is much reduced compared to CHSY1 (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan = UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan.
UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.

Cofactori

Co2+By similarity, Mn2+By similarity, Cd2+By similarityNote: Divalent metal cations. Highest activities are measured with Co2+, Mn2+ and Cd2+.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi722 – 7221Divalent metal cationSequence analysis
Metal bindingi836 – 8361Divalent metal cationSequence analysis

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2022870. Chondroitin sulfate biosynthesis.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.
GT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Chondroitin sulfate synthase 3 (EC:2.4.1.175, EC:2.4.1.226)
Alternative name(s):
Carbohydrate synthase 2
Chondroitin glucuronyltransferase 3
Chondroitin synthase 2
Short name:
ChSy-2
Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II
N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 3
N-acetylgalactosaminyltransferase 3
Gene namesi
Name:Chsy3
Synonyms:Chsy2, Css3, Kiaa4168
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1926173. Chsy3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence analysis
Transmembranei8 – 2821Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini29 – 884856LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 884884Chondroitin sulfate synthase 3PRO_0000320145Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi155 – 1551N-linked (GlcNAc...)Sequence analysis
Glycosylationi281 – 2811N-linked (GlcNAc...)Sequence analysis
Glycosylationi712 – 7121N-linked (GlcNAc...)Sequence analysis
Glycosylationi880 – 8801N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ5DTK1.
PaxDbiQ5DTK1.
PRIDEiQ5DTK1.

PTM databases

PhosphoSiteiQ5DTK1.

Expressioni

Gene expression databases

BgeeiQ5DTK1.
CleanExiMM_CHSY3.
GenevisibleiQ5DTK1. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000079546.

Structurei

3D structure databases

ProteinModelPortaliQ5DTK1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi211 – 23727Pro-richAdd
BLAST
Compositional biasi541 – 5466Poly-Leu

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3588. Eukaryota.
ENOG410XNYM. LUCA.
GeneTreeiENSGT00760000119143.
HOGENOMiHOG000220809.
HOVERGENiHBG050948.
InParanoidiQ5DTK1.
KOiK13499.
OMAiDRVNCPR.
OrthoDBiEOG74TWXX.
TreeFamiTF318303.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR008428. Chond_GalNAc.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF05679. CHGN. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 3 hits.

Sequencei

Sequence statusi: Complete.

Q5DTK1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVRSRRPWV SVALGLVLGF TAASWLIAPR VAELSEKRRR GSSLCSYYGR
60 70 80 90 100
SATGPRADAQ QLLPQPQSRP RLEQSPPPAS HELPGPQQPE AAPGGPSFRS
110 120 130 140 150
SPWQQPALLP QRRRGHTPEG ATALPGAPAA KGEPEEEDGG AADPRKGGRP
160 170 180 190 200
GSSHNGSGDG GAAVPTSGPG DFLYVGVMTA QKYLGSRALA AQRTWARFIP
210 220 230 240 250
GRVEFFSSQQ SPSAALGQPP PPLPVIALPG VDDSYPPQKK SFMMIKYMHD
260 270 280 290 300
HYLDKYEWFM RADDDVYIKG DKLEEFLRSL NSSKPLYLGQ TGLGNTEELG
310 320 330 340 350
KLGLEPGENF CMGGPGMIFS REVLRRMVPH IGECLREMYT THEDVEVGRC
360 370 380 390 400
VRRFGGTQCV WSYEMQQLFH ENYEHNRKGY IQDLHNSKIH AAITLHPNKR
410 420 430 440 450
PAYQYRLHNY MLSRKISELR YRTIQLHRES ALMSKLSNSE VSKEDQQLGR
460 470 480 490 500
TPSFNHFQPR ERNEVMEWEF LTGKLLYSAA ENQPPRQSIN SILRSALDDT
510 520 530 540 550
VLQVMEMINE NAKSRGRLID FKEIQYGYRR VDPMHGVEYI LDLLLLYKRH
560 570 580 590 600
KGRKLTVPVR RHAYLQQPFS KPFFREVEEL DVNRLVESIN SGTQSFSVIS
610 620 630 640 650
NSLKILSSLQ EAKDIGGHNE KKVHILVPLV GRYDIFLRFM ENFESTCLIP
660 670 680 690 700
KQNVKLVIIL FSRDAGQESI KHIELIQEYQ SRYPSAEMML IPMKGEFSRG
710 720 730 740 750
LGLEMASSQF DNDTLLLFCD VDLIFRGDFL QRCRDNTVQG QQVYYPIIFS
760 770 780 790 800
QYDPKVTHMR NPPTEGDFVF SKETGFWRDY GYGITCIYKS DLLGAGGFDT
810 820 830 840 850
SIQGWGLEDV DLYNKVILSG LRPFRSQEVG VVHIFHPVHC DPNLDPKQYK
860 870 880
MCLGSKASTF ASTMQLAELW LEKHLGVRDN RTLS
Length:884
Mass (Da):100,099
Last modified:July 27, 2011 - v3
Checksum:iD1D9E9EAA6D2FB3E
GO

Sequence cautioni

The sequence BAD90307.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti108 – 1081L → P in BAD90307 (Ref. 1) Curated
Sequence conflicti164 – 1641V → A in BAD90307 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220519 mRNA. Translation: BAD90307.1. Different initiation.
CH466528 Genomic DNA. Translation: EDL09842.1.
BC151164 mRNA. Translation: AAI51165.1.
BC151165 mRNA. Translation: AAI51166.1.
BC157985 mRNA. Translation: AAI57986.1.
CCDSiCCDS37829.1.
RefSeqiNP_001074797.1. NM_001081328.1.
UniGeneiMm.485185.
Mm.84007.

Genome annotation databases

EnsembliENSMUST00000080721; ENSMUSP00000079546; ENSMUSG00000058152.
GeneIDi78923.
KEGGimmu:78923.
UCSCiuc008ezw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220519 mRNA. Translation: BAD90307.1. Different initiation.
CH466528 Genomic DNA. Translation: EDL09842.1.
BC151164 mRNA. Translation: AAI51165.1.
BC151165 mRNA. Translation: AAI51166.1.
BC157985 mRNA. Translation: AAI57986.1.
CCDSiCCDS37829.1.
RefSeqiNP_001074797.1. NM_001081328.1.
UniGeneiMm.485185.
Mm.84007.

3D structure databases

ProteinModelPortaliQ5DTK1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000079546.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.
GT7. Glycosyltransferase Family 7.

PTM databases

PhosphoSiteiQ5DTK1.

Proteomic databases

MaxQBiQ5DTK1.
PaxDbiQ5DTK1.
PRIDEiQ5DTK1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000080721; ENSMUSP00000079546; ENSMUSG00000058152.
GeneIDi78923.
KEGGimmu:78923.
UCSCiuc008ezw.1. mouse.

Organism-specific databases

CTDi337876.
MGIiMGI:1926173. Chsy3.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG3588. Eukaryota.
ENOG410XNYM. LUCA.
GeneTreeiENSGT00760000119143.
HOGENOMiHOG000220809.
HOVERGENiHBG050948.
InParanoidiQ5DTK1.
KOiK13499.
OMAiDRVNCPR.
OrthoDBiEOG74TWXX.
TreeFamiTF318303.

Enzyme and pathway databases

ReactomeiR-MMU-2022870. Chondroitin sulfate biosynthesis.

Miscellaneous databases

NextBioi349766.
PROiQ5DTK1.
SOURCEiSearch...

Gene expression databases

BgeeiQ5DTK1.
CleanExiMM_CHSY3.
GenevisibleiQ5DTK1. MM.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR008428. Chond_GalNAc.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF05679. CHGN. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 3 hits.
ProtoNetiSearch...

Publicationsi

  1. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Fetal brain.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiCHSS3_MOUSE
AccessioniPrimary (citable) accession number: Q5DTK1
Secondary accession number(s): B2RXU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: July 27, 2011
Last modified: November 11, 2015
This is version 78 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.