Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ectonucleoside triphosphate diphosphohydrolase 8

Gene

Entpd8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Canalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity. Ectonucleoside NTPDases catalyze the hydrolysis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentration of extracellular nucleotides. Has activity toward ATP, ADP, UTP and UDP, but not toward AMP (By similarity).By similarity

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

Ca2+By similarity, Mg2+By similarityNote: Ca(2+) or Mg(2+). Has lower efficiency with Mg2+.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei168 – 1681Proton acceptorBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrolase activity Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleoside triphosphate diphosphohydrolase 8 (EC:3.6.1.5)
Short name:
E-NTPDase 8
Short name:
NTPDase 8
Short name:
NTPDase8
Gene namesi
Name:Entpd8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi1561793. Entpd8.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88CytoplasmicSequence Analysis
Transmembranei9 – 2921HelicalSequence AnalysisAdd
BLAST
Topological domaini30 – 465436ExtracellularSequence AnalysisAdd
BLAST
Transmembranei466 – 48621HelicalSequence AnalysisAdd
BLAST
Topological domaini487 – 4948CytoplasmicSequence Analysis

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Ectonucleoside triphosphate diphosphohydrolase 8PRO_0000306884Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi78 ↔ 102By similarity
Disulfide bondi245 ↔ 291By similarity
Glycosylationi299 – 2991N-linked (GlcNAc...)Sequence Analysis
Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi328 ↔ 334By similarity
Glycosylationi362 – 3621N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi380 ↔ 402By similarity

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ5DRK1.
PRIDEiQ5DRK1.

Expressioni

Tissue specificityi

Present in liver, and at lower level in jejunum and kidney. Limited to the canalicular domain of hepathocytes (at protein level).1 Publication

Gene expression databases

GenevestigatoriQ5DRK1.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012289.

Structurei

3D structure databases

ProteinModelPortaliQ5DRK1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The transmembranous domains are involved in regulation of enzyme activity.By similarity

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5371.
HOGENOMiHOG000059572.
HOVERGENiHBG018982.
InParanoidiQ5DRK1.
KOiK01510.
PhylomeDBiQ5DRK1.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5DRK1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLSWKERVF MVLLGVAAAS GLTMLILILV KATNVLLPAD TKFGILFDAG
60 70 80 90 100
SSHTSLFVYQ WPANKEKDTG VVSQALACQV EGPGISSYTS DPTQAGESLK
110 120 130 140 150
SCLQEALALI PQTQHPVTPA FLGATAGMRL LSQKNSSQAQ DILAAVSQTL
160 170 180 190 200
SRAPVDFWGA RILAGQDEGA FGWITVNYVL GMLLKYSSGQ WILPEDGTLV
210 220 230 240 250
GALDLGGAST QISFVPQGPI LDQSTQVTFR LYGANYSVYT HSYLCFGRDQ
260 270 280 290 300
ILRRLLAELV QSSQVARVRH PCYHSGYQAT LSLASLYDSP CVHTPDSLNY
310 320 330 340 350
TQNLTVEGIG NPGNCVAALR GLFNFSSCKG QEDCAFNGVY QPPVHGQFYA
360 370 380 390 400
FSNFYYTFQF LNLTSRQPLN IVNDTIWKFC QKPWRLVEDS YPGQERWLRD
410 420 430 440 450
YCASGLYILV LLLEGYKFSE ETWPNIQFQK QAGGTDIGWT LGFMLNLTGM
460 470 480 490
IPAEALTQWR AQSYSIWIAG VVFAVLTLVA ILGAAAVQLF WTQD
Length:494
Mass (Da):54,330
Last modified:March 29, 2005 - v1
Checksum:i1261896D24D06458
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY536920 mRNA. Translation: AAT08794.1.
RefSeqiNP_001028737.1. NM_001033565.1.
UniGeneiRn.160067.

Genome annotation databases

GeneIDi613267.
KEGGirno:613267.
UCSCiRGD:1561793. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY536920 mRNA. Translation: AAT08794.1.
RefSeqiNP_001028737.1. NM_001033565.1.
UniGeneiRn.160067.

3D structure databases

ProteinModelPortaliQ5DRK1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012289.

Proteomic databases

PaxDbiQ5DRK1.
PRIDEiQ5DRK1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi613267.
KEGGirno:613267.
UCSCiRGD:1561793. rat.

Organism-specific databases

CTDi377841.
RGDi1561793. Entpd8.

Phylogenomic databases

eggNOGiCOG5371.
HOGENOMiHOG000059572.
HOVERGENiHBG018982.
InParanoidiQ5DRK1.
KOiK01510.
PhylomeDBiQ5DRK1.

Miscellaneous databases

NextBioi714602.

Gene expression databases

GenevestigatoriQ5DRK1.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning, purification, and identification of the liver canalicular ecto-ATPase as NTPDase8."
    Fausther M., Lecka J., Kukulski F., Levesque S.A., Pelletier J., Zimmermann H., Dranoff J.A., Sevigny J.
    Am. J. Physiol. 292:G785-G795(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Liver.

Entry informationi

Entry nameiENTP8_RAT
AccessioniPrimary (citable) accession number: Q5DRK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: March 29, 2005
Last modified: January 7, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.