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Q5DRK1

- ENTP8_RAT

UniProt

Q5DRK1 - ENTP8_RAT

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Protein

Ectonucleoside triphosphate diphosphohydrolase 8

Gene
Entpd8
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Canalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity. Ectonucleoside NTPDases catalyze the hydrolysis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentration of extracellular nucleotides. Has activity toward ATP, ADP, UTP and UDP, but not toward AMP By similarity.

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

Ca2+ or Mg2+. Has lower efficiency with Mg2+ By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei168 – 1681Proton acceptor By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrolase activity Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleoside triphosphate diphosphohydrolase 8 (EC:3.6.1.5)
Short name:
E-NTPDase 8
Short name:
NTPDase 8
Short name:
NTPDase8
Gene namesi
Name:Entpd8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi1561793. Entpd8.

Subcellular locationi

Cell membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88Cytoplasmic Reviewed prediction
Transmembranei9 – 2921Helical; Reviewed predictionAdd
BLAST
Topological domaini30 – 465436Extracellular Reviewed predictionAdd
BLAST
Transmembranei466 – 48621Helical; Reviewed predictionAdd
BLAST
Topological domaini487 – 4948Cytoplasmic Reviewed prediction

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Ectonucleoside triphosphate diphosphohydrolase 8PRO_0000306884Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi78 ↔ 102 By similarity
Disulfide bondi245 ↔ 291 By similarity
Glycosylationi299 – 2991N-linked (GlcNAc...) Reviewed prediction
Glycosylationi303 – 3031N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi328 ↔ 334 By similarity
Glycosylationi362 – 3621N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi380 ↔ 402 By similarity

Post-translational modificationi

N-glycosylated By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ5DRK1.
PRIDEiQ5DRK1.

Expressioni

Tissue specificityi

Present in liver, and at lower level in jejunum and kidney. Limited to the canalicular domain of hepathocytes (at protein level).1 Publication

Gene expression databases

GenevestigatoriQ5DRK1.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012289.

Structurei

3D structure databases

ProteinModelPortaliQ5DRK1.

Family & Domainsi

Domaini

The transmembranous domains are involved in regulation of enzyme activity By similarity.

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5371.
HOGENOMiHOG000059572.
HOVERGENiHBG018982.
InParanoidiQ5DRK1.
KOiK01510.
PhylomeDBiQ5DRK1.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5DRK1-1 [UniParc]FASTAAdd to Basket

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MRLSWKERVF MVLLGVAAAS GLTMLILILV KATNVLLPAD TKFGILFDAG    50
SSHTSLFVYQ WPANKEKDTG VVSQALACQV EGPGISSYTS DPTQAGESLK 100
SCLQEALALI PQTQHPVTPA FLGATAGMRL LSQKNSSQAQ DILAAVSQTL 150
SRAPVDFWGA RILAGQDEGA FGWITVNYVL GMLLKYSSGQ WILPEDGTLV 200
GALDLGGAST QISFVPQGPI LDQSTQVTFR LYGANYSVYT HSYLCFGRDQ 250
ILRRLLAELV QSSQVARVRH PCYHSGYQAT LSLASLYDSP CVHTPDSLNY 300
TQNLTVEGIG NPGNCVAALR GLFNFSSCKG QEDCAFNGVY QPPVHGQFYA 350
FSNFYYTFQF LNLTSRQPLN IVNDTIWKFC QKPWRLVEDS YPGQERWLRD 400
YCASGLYILV LLLEGYKFSE ETWPNIQFQK QAGGTDIGWT LGFMLNLTGM 450
IPAEALTQWR AQSYSIWIAG VVFAVLTLVA ILGAAAVQLF WTQD 494
Length:494
Mass (Da):54,330
Last modified:March 29, 2005 - v1
Checksum:i1261896D24D06458
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY536920 mRNA. Translation: AAT08794.1.
RefSeqiNP_001028737.1. NM_001033565.1.
UniGeneiRn.160067.

Genome annotation databases

GeneIDi613267.
KEGGirno:613267.
UCSCiRGD:1561793. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY536920 mRNA. Translation: AAT08794.1 .
RefSeqi NP_001028737.1. NM_001033565.1.
UniGenei Rn.160067.

3D structure databases

ProteinModelPortali Q5DRK1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000012289.

Proteomic databases

PaxDbi Q5DRK1.
PRIDEi Q5DRK1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 613267.
KEGGi rno:613267.
UCSCi RGD:1561793. rat.

Organism-specific databases

CTDi 377841.
RGDi 1561793. Entpd8.

Phylogenomic databases

eggNOGi COG5371.
HOGENOMi HOG000059572.
HOVERGENi HBG018982.
InParanoidi Q5DRK1.
KOi K01510.
PhylomeDBi Q5DRK1.

Miscellaneous databases

NextBioi 714602.

Gene expression databases

Genevestigatori Q5DRK1.

Family and domain databases

InterProi IPR000407. GDA1_CD39_NTPase.
[Graphical view ]
PANTHERi PTHR11782. PTHR11782. 1 hit.
Pfami PF01150. GDA1_CD39. 1 hit.
[Graphical view ]
PROSITEi PS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, purification, and identification of the liver canalicular ecto-ATPase as NTPDase8."
    Fausther M., Lecka J., Kukulski F., Levesque S.A., Pelletier J., Zimmermann H., Dranoff J.A., Sevigny J.
    Am. J. Physiol. 292:G785-G795(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Liver.

Entry informationi

Entry nameiENTP8_RAT
AccessioniPrimary (citable) accession number: Q5DRK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: March 29, 2005
Last modified: April 16, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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