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Q5DRK1

- ENTP8_RAT

UniProt

Q5DRK1 - ENTP8_RAT

Protein

Ectonucleoside triphosphate diphosphohydrolase 8

Gene

Entpd8

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (29 Mar 2005)
      Previous versions | rss
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    Functioni

    Canalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity. Ectonucleoside NTPDases catalyze the hydrolysis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentration of extracellular nucleotides. Has activity toward ATP, ADP, UTP and UDP, but not toward AMP By similarity.By similarity

    Catalytic activityi

    A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

    Cofactori

    Ca2+ or Mg2+. Has lower efficiency with Mg2+ By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei168 – 1681Proton acceptorBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. hydrolase activity Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ectonucleoside triphosphate diphosphohydrolase 8 (EC:3.6.1.5)
    Short name:
    E-NTPDase 8
    Short name:
    NTPDase 8
    Short name:
    NTPDase8
    Gene namesi
    Name:Entpd8
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi1561793. Entpd8.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 494494Ectonucleoside triphosphate diphosphohydrolase 8PRO_0000306884Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi78 ↔ 102By similarity
    Disulfide bondi245 ↔ 291By similarity
    Glycosylationi299 – 2991N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi328 ↔ 334By similarity
    Glycosylationi362 – 3621N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi380 ↔ 402By similarity

    Post-translational modificationi

    N-glycosylated.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ5DRK1.
    PRIDEiQ5DRK1.

    Expressioni

    Tissue specificityi

    Present in liver, and at lower level in jejunum and kidney. Limited to the canalicular domain of hepathocytes (at protein level).1 Publication

    Gene expression databases

    GenevestigatoriQ5DRK1.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000012289.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5DRK1.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 88CytoplasmicSequence Analysis
    Topological domaini30 – 465436ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini487 – 4948CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei9 – 2921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei466 – 48621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domaini

    The transmembranous domains are involved in regulation of enzyme activity.By similarity

    Sequence similaritiesi

    Belongs to the GDA1/CD39 NTPase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5371.
    HOGENOMiHOG000059572.
    HOVERGENiHBG018982.
    InParanoidiQ5DRK1.
    KOiK01510.
    PhylomeDBiQ5DRK1.

    Family and domain databases

    InterProiIPR000407. GDA1_CD39_NTPase.
    [Graphical view]
    PANTHERiPTHR11782. PTHR11782. 1 hit.
    PfamiPF01150. GDA1_CD39. 1 hit.
    [Graphical view]
    PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5DRK1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRLSWKERVF MVLLGVAAAS GLTMLILILV KATNVLLPAD TKFGILFDAG    50
    SSHTSLFVYQ WPANKEKDTG VVSQALACQV EGPGISSYTS DPTQAGESLK 100
    SCLQEALALI PQTQHPVTPA FLGATAGMRL LSQKNSSQAQ DILAAVSQTL 150
    SRAPVDFWGA RILAGQDEGA FGWITVNYVL GMLLKYSSGQ WILPEDGTLV 200
    GALDLGGAST QISFVPQGPI LDQSTQVTFR LYGANYSVYT HSYLCFGRDQ 250
    ILRRLLAELV QSSQVARVRH PCYHSGYQAT LSLASLYDSP CVHTPDSLNY 300
    TQNLTVEGIG NPGNCVAALR GLFNFSSCKG QEDCAFNGVY QPPVHGQFYA 350
    FSNFYYTFQF LNLTSRQPLN IVNDTIWKFC QKPWRLVEDS YPGQERWLRD 400
    YCASGLYILV LLLEGYKFSE ETWPNIQFQK QAGGTDIGWT LGFMLNLTGM 450
    IPAEALTQWR AQSYSIWIAG VVFAVLTLVA ILGAAAVQLF WTQD 494
    Length:494
    Mass (Da):54,330
    Last modified:March 29, 2005 - v1
    Checksum:i1261896D24D06458
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY536920 mRNA. Translation: AAT08794.1.
    RefSeqiNP_001028737.1. NM_001033565.1.
    UniGeneiRn.160067.

    Genome annotation databases

    GeneIDi613267.
    KEGGirno:613267.
    UCSCiRGD:1561793. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY536920 mRNA. Translation: AAT08794.1 .
    RefSeqi NP_001028737.1. NM_001033565.1.
    UniGenei Rn.160067.

    3D structure databases

    ProteinModelPortali Q5DRK1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000012289.

    Proteomic databases

    PaxDbi Q5DRK1.
    PRIDEi Q5DRK1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 613267.
    KEGGi rno:613267.
    UCSCi RGD:1561793. rat.

    Organism-specific databases

    CTDi 377841.
    RGDi 1561793. Entpd8.

    Phylogenomic databases

    eggNOGi COG5371.
    HOGENOMi HOG000059572.
    HOVERGENi HBG018982.
    InParanoidi Q5DRK1.
    KOi K01510.
    PhylomeDBi Q5DRK1.

    Miscellaneous databases

    NextBioi 714602.

    Gene expression databases

    Genevestigatori Q5DRK1.

    Family and domain databases

    InterProi IPR000407. GDA1_CD39_NTPase.
    [Graphical view ]
    PANTHERi PTHR11782. PTHR11782. 1 hit.
    Pfami PF01150. GDA1_CD39. 1 hit.
    [Graphical view ]
    PROSITEi PS01238. GDA1_CD39_NTPASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, purification, and identification of the liver canalicular ecto-ATPase as NTPDase8."
      Fausther M., Lecka J., Kukulski F., Levesque S.A., Pelletier J., Zimmermann H., Dranoff J.A., Sevigny J.
      Am. J. Physiol. 292:G785-G795(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Liver.

    Entry informationi

    Entry nameiENTP8_RAT
    AccessioniPrimary (citable) accession number: Q5DRK1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: March 29, 2005
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3