Q5DRK1 (ENTP8_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ectonucleoside triphosphate diphosphohydrolase 8
Short name=E-NTPDase 8
Short name=NTPDase 8
Short name=NTPDase8
EC=3.6.1.5

Gene names

Name:

Entpd8

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Canalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity. Ectonucleoside NTPDases catalyze the hydrolysis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentration of extracellular nucleotides. Has activity toward ATP, ADP, UTP and UDP, but not toward AMP By similarity.
Catalytic activity	ATP + 2 H2O = AMP + 2 phosphate.
Cofactor	Calcium By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Ref.1.
Tissue specificity	Present in liver, and at lower level in jejunum and kidney. Limited to the canalicular domain of hepathocytes (at protein level). Ref.1
Domain	The transmembranous domains are involved in regulation of enzyme activity By similarity.
Post-translational modification	N-glycosylated By similarity.
Sequence similarities	Belongs to the GDA1/CD39 NTPase family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Calcium Nucleotide-binding
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	Ectonucleoside triphosphate diphosphohydrolase 8		PRO_0000306884
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 29	21	Helical; Potential
	Topological domain	30 – 465	436	Extracellular Potential
	Transmembrane	466 – 486	21	Helical; Potential
	Topological domain	487 – 494	8	Cytoplasmic Potential
Sites
	Active site	168	1	Proton acceptor By similarity
Amino acid modifications
	Glycosylation	299	1	N-linked (GlcNAc...) Potential
	Glycosylation	303	1	N-linked (GlcNAc...) Potential
	Glycosylation	362	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5DRK1 [UniParc]. Last modified March 29, 2005. Version 1. Checksum: 1261896D24D06458	FASTA	494	54,330
	10 20 30 40 50 60 MRLSWKERVF MVLLGVAAAS GLTMLILILV KATNVLLPAD TKFGILFDAG SSHTSLFVYQ 70 80 90 100 110 120 WPANKEKDTG VVSQALACQV EGPGISSYTS DPTQAGESLK SCLQEALALI PQTQHPVTPA 130 140 150 160 170 180 FLGATAGMRL LSQKNSSQAQ DILAAVSQTL SRAPVDFWGA RILAGQDEGA FGWITVNYVL 190 200 210 220 230 240 GMLLKYSSGQ WILPEDGTLV GALDLGGAST QISFVPQGPI LDQSTQVTFR LYGANYSVYT 250 260 270 280 290 300 HSYLCFGRDQ ILRRLLAELV QSSQVARVRH PCYHSGYQAT LSLASLYDSP CVHTPDSLNY 310 320 330 340 350 360 TQNLTVEGIG NPGNCVAALR GLFNFSSCKG QEDCAFNGVY QPPVHGQFYA FSNFYYTFQF 370 380 390 400 410 420 LNLTSRQPLN IVNDTIWKFC QKPWRLVEDS YPGQERWLRD YCASGLYILV LLLEGYKFSE 430 440 450 460 470 480 ETWPNIQFQK QAGGTDIGWT LGFMLNLTGM IPAEALTQWR AQSYSIWIAG VVFAVLTLVA 490 ILGAAAVQLF WTQD

References

[1]

"Cloning, purification, and identification of the liver canalicular ecto-ATPase as NTPDase8." Fausther M., Lecka J., Kukulski F., Levesque S.A., Pelletier J., Zimmermann H., Dranoff J.A., Sevigny J. Am. J. Physiol. 292:G785-G795(2007) [PubMed: 17095758] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY536920 mRNA. Translation: AAT08794.1.
IPI	IPI00369830.
RefSeq	NP_001028737.1. NM_001033565.1.
UniGene	Rn.160067.
3D structure databases
ProteinModelPortal	Q5DRK1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5DRK1.
Proteomic databases
PRIDE	Q5DRK1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	613267.
KEGG	rno:613267.
Organism-specific databases
CTD	377841.
RGD	1561793. Entpd8.
Phylogenomic databases
eggNOG	roNOG14650.
GeneTree	ENSGT00550000074435.
HOVERGEN	HBG018982.
InParanoid	Q5DRK1.
OrthoDB	EOG48WC1R.
PhylomeDB	Q5DRK1.
Gene expression databases
Genevestigator	Q5DRK1.
Family and domain databases
InterPro	IPR000407. GDA1_CD39_NTPase. [Graphical view]
KO	K01510.
PANTHER	PTHR11782. GDA1_CD39_NTPase. 1 hit.
Pfam	PF01150. GDA1_CD39. 1 hit. [Graphical view]
PROSITE	PS01238. GDA1_CD39_NTPASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	714602.

Entry information

Entry name

ENTP8_RAT

Accession

Primary (citable) accession number: Q5DRK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	March 29, 2005
Last modified:	November 16, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program