Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Hemagglutinin

Gene

HA

Organism
Influenza A virus (A/black-headed gull/Sweden/2/99(H16N3))
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.UniRule annotationSAAS annotation
Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHemagglutininUniRule annotationSAAS annotation
Biological processClathrin- and caveolin-independent endocytosis of virus by hostUniRule annotationSAAS annotation, Clathrin-mediated endocytosis of virus by hostUniRule annotationSAAS annotation, Fusion of virus membrane with host endosomal membraneUniRule annotationSAAS annotation, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cellUniRule annotationSAAS annotation, Viral penetration into host cytoplasm, Virus endocytosis by host, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
HemagglutininUniRule annotation
Cleaved into the following 2 chains:
Hemagglutinin HA2 chainUniRule annotation
Hemagglutinin HA1 chainUniRule annotation
Gene namesi
Name:HAUniRule annotationImported
OrganismiInfluenza A virus (A/black-headed gull/Sweden/2/99(H16N3))Imported
Taxonomic identifieri304362 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A

Subcellular locationi

  • Host apical cell membrane UniRule annotationSAAS annotation; Single-pass type I membrane protein UniRule annotationSAAS annotation
  • Virion membrane UniRule annotation; Single-pass type I membrane protein UniRule annotation
  • Note: Targeted to the apical plasma membrane in epithelial polarized cells through a signal present in the transmembrane domain. Associated with glycosphingolipid- and cholesterol-enriched detergent-resistant lipid rafts.UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei529 – 551HelicalUniRule annotationAdd BLAST23

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membraneUniRule annotationSAAS annotation, Host membrane, Membrane, Viral envelope proteinUniRule annotationSAAS annotation, Virion

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi22 ↔ 478Combined sources
Disulfide bondi60 ↔ 289Combined sources
Disulfide bondi73 ↔ 85UniRule annotationCombined sources
Disulfide bondi108 ↔ 151Combined sources
Disulfide bondi293 ↔ 317UniRule annotationCombined sources
Disulfide bondi485 ↔ 489UniRule annotationCombined sources
Lipidationi553S-palmitoyl cysteine; by hostUniRule annotation1
Lipidationi563S-palmitoyl cysteine; by hostUniRule annotation1

Post-translational modificationi

In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. One identified protease that may be involved in this process is secreted in lungs by Clara cells.UniRule annotation
Palmitoylated.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei341 – 342Cleavage; by hostUniRule annotation2

Keywords - PTMi

Disulfide bondUniRule annotationSAAS annotation, GlycoproteinUniRule annotation, Lipoprotein, PalmitateUniRule annotation

Interactioni

Subunit structurei

Homotrimer of disulfide-linked HA1-HA2.UniRule annotationSAAS annotation

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4F23X-ray1.70A/B/C14-519[»]
4FIUX-ray2.00A/B/C14-519[»]
ProteinModelPortaliQ5DL24.
SMRiQ5DL24.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili397 – 417Sequence analysisAdd BLAST21
Coiled coili449 – 469Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the influenza viruses hemagglutinin family.UniRule annotationSAAS annotation

Keywords - Domaini

Coiled coilSequence analysis, SignalUniRule annotation, Transmembrane, Transmembrane helixUniRule annotationSAAS annotation

Family and domain databases

Gene3Di3.90.209.20. 1 hit.
HAMAPiMF_04072. INFV_HEMA. 1 hit.
InterProiView protein in InterPro
IPR008980. Capsid_hemagglutn.
IPR013828. Hemagglutn_HA1_a/b_dom_sf.
IPR000149. Hemagglutn_influenz_A.
IPR001364. Hemagglutn_influenz_A/B.
PfamiView protein in Pfam
PF00509. Hemagglutinin. 1 hit.
PRINTSiPR00330. HEMAGGLUTN1.
PR00329. HEMAGGLUTN12.
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5DL24-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKTLYLLVL IFVKYSRADK ICIGYLSNNS TDTVDTLTEN GVPVTSSIDL
60 70 80 90 100
VETNHTGTYC SLNGVSPIHL GDCSFEGWIV GNPSCASNIN IREWSYLIED
110 120 130 140 150
PNAPHKLCFP GEVDNNGELR HLFSGVNSFS RTELIPPSKW GDILEGTTAS
160 170 180 190 200
CQNRGANSFY RNLIWLVNKL NKYPVVKGEY NNTTGRDVLV LWGIHHPDTE
210 220 230 240 250
ATANKLYVNK NPYTLVSTKE WSRRYELEIG TRIGDGQRSW MKIYWHLMHP
260 270 280 290 300
GERITFESSG GLLAPRYGYI IEKYGTGRIF QSGVRLAKCN TKCQTSMGGI
310 320 330 340 350
NTNKTFQNIE RNALGDCPKY IKSGQLKLAT GLRNVPSIVE RGLFGAIAGF
360 370 380 390 400
IEGGWPGLIN GWYGFQHQNE QGTGIAADKT STQKAINEIT TKINNIIEKM
410 420 430 440 450
NGNYDSIRGE FNQVEKRINM IADRVDDAVT DIWSYNAKLL VLIENDRTLD
460 470 480 490 500
LHDANVRNLH EQIKRALKDN AIDEGDGCFS ILHKCNDSCM ETIRNGTYNH
510 520 530 540 550
EDYKEESQLK RQEIEGIKLK TEDNVYKILS IYSCIASSVV LVGLILAFIL
560
WACSSGNCRF NVCI
Length:564
Mass (Da):63,266
Last modified:March 29, 2005 - v1
Checksum:i24ECBDF6D2233971
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY684888 Genomic RNA. Translation: AAV91214.1.

Similar proteinsi

Entry informationi

Entry nameiQ5DL24_9INFA
AccessioniPrimary (citable) accession number: Q5DL24
Entry historyiIntegrated into UniProtKB/TrEMBL: March 29, 2005
Last sequence update: March 29, 2005
Last modified: January 31, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

3D-structureCombined sources