Q5D7H4 (HUGAB_VESVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Inactive hyaluronidase B Short name=Hya B Alternative name(s): Hyaluronoglucosaminidase B Allergen=Ves v 2b
Organism	Vespula vulgaris (Yellow jacket) (Wasp)
Taxonomic identifier	7454 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Vespoidea › Vespidae › Vespinae › Vespula

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has no hyaluronidase activity. Ref.2
Subcellular location	Secreted Ref.1.
Tissue specificity	Expressed by the venom gland. Ref.1
Post-translational modification	N-glycosylated on at least two Asn residues by identical heptasaccharide units composed of Man, GlcNAc, and Fuc residues in the molar ration of 3:2:2. Ref.1
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Belongs to the glycosyl hydrolase 56 family.
Caution	Lacks the typical Glu active site in position 111 that is replaced by a His residue, preventing the hyaluronidase activity.

Ontologies

Keywords
Cellular component	Secreted
Disease	Allergen
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	hyalurononglucosaminidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Inactive hyaluronidase B		PRO_0000271764
Amino acid modifications
	Glycosylation	66	1	N-linked (GlcNAc...) Ref.1
	Glycosylation	81	1	N-linked (GlcNAc...) Ref.1
	Disulfide bond	21 ↔ 310		By similarity UniProtKB Q08169
	Disulfide bond	187 ↔ 199		By similarity UniProtKB Q08169
Experimental info
	Sequence conflict	10	1	F → S AA sequence Ref.1
	Sequence conflict	273	1	I → L AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q5D7H4 [UniParc]. Last modified March 29, 2005. Version 1. Checksum: 9E922056BCFC1CA1	FASTA	340	40,072
	10 20 30 40 50 60 DRTIWPKKGF SIYWNIPTHF CHNFGVYFKE LKQFNIKYNS MNNFRGETIS LFYDPGNFPS 70 80 90 100 110 120 MVLLKNGTYE IRNEGVPQKG NLTIHLEQFT KELDEIYPKK IAGGIGVIHF HNWRPIFRRN 130 140 150 160 170 180 VDNLKINKDI SIDLVRKEHP KWDKSMIEKE ASNRFETSAK IFMEKTLKLA KEIRKKTEWG 190 200 210 220 230 240 YHGYPHCLSG STDKPSFDCD ALSMSENDKM SWLFNNQNVL LPSIYLKNVL KPDEKIHLVQ 250 260 270 280 290 300 ERLKEAIRIS KNFKHLPKVL PYWWYTYQDK ESIFLTEADV KNTFKEILTN GADGIIIWGV 310 320 330 340 SYELTDRKRC EKLKEYLMKI LGPIAFKVTK AVKENTPLNF

References

[1]

"The N-glycans of yellow jacket venom hyaluronidases and the protein sequence of its major isoform in Vespula vulgaris." Kolarich D., Leonard R., Hemmer W., Altmann F. FEBS J. 272:5182-5190(2005) [PubMed: 16218950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-15; 53-59; 92-99; 129-136; 228-242; 259-281 AND 320-327, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION AT ASN-66 AND ASN-81. Tissue: Venom and Venom gland.

[2]

"Dissecting cross-reactivity in hymenoptera venom allergy by circumvention of alpha-1,3-core fucosylation." Seismann H., Blank S., Braren I., Greunke K., Cifuentes L., Grunwald T., Bredehorst R., Ollert M., Spillner E. Mol. Immunol. 47:799-808(2010) [PubMed: 19896717] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY845866 mRNA. Translation: AAX14718.1. AJ920395 mRNA. Translation: CAI77218.1.
3D structure databases
HSSP	HSSP built from PDB template 2ATM based on UniProtKB P49370.
ProteinModelPortal	Q5D7H4.
SMR	Q5D7H4. Positions 8-331.
ModBase	Search...
Protein family/group databases
Allergome	3522. Ves v 2.0201. 669. Ves v 2.
CAZy	GH56. Glycoside Hydrolase Family 56.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR001329. Glyco_hydro_56_Api/Dol. IPR017853. Glycoside_hydrolase_SF. IPR018155. Hyaluronidase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11769. Glyco_hydro_56. 1 hit. PTHR11769:SF2. Glyco_hydro_56_Api/Dol. 1 hit.
Pfam	PF01630. Glyco_hydro_56. 1 hit. [Graphical view]
PRINTS	PR00846. GLHYDRLASE56. PR00847. HYALURONDASE.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUGAB_VESVU

Accession

Primary (citable) accession number: Q5D7H4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	March 29, 2005
Last modified:	May 31, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)