Q5D1E8 (ZC12A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease ZC3H12A
EC=3.1.-.-

Alternative name(s):
MCP-induced protein 1
Zinc finger CCCH domain-containing protein 12A

Gene names

Name:	ZC3H12A
Synonyms:	MCPIP, MCPIP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	599 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has RNase activity and selectively degrades specific target mRNA species. Modulates the immune response and inflammation by regulating the decay of specific mRNA molecules. Recognizes the 3'-untranslated region (UTR) of the mRNA for IL6, CALCR and IL12B. Required for normal decay of IL6 mRNA By similarity. Triggers apoptosis and promotes angiogenesis in response to the binding of CCL2 to CCR2. Regulates expression of CDH12 and CHD19. Ref.1 Ref.8
Cofactor	Magnesium By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus Ref.1.
Induction	By cytokines (TNF-alpha and interleukin-1) in acute monocytic leukemia cell line THP-1 cells. Ref.7
Miscellaneous	Increased expression of ZC3H12A is associated with ischemic heart disease.
Sequence similarities	Belongs to the ZC3H12 family. Contains 1 C3H1-type zinc finger. UniProtKB A6QQJ8
Caution	Was originally (Ref.8) proposed to bind to DNA and act as transcription factor.

Ontologies

Keywords
Biological process	Angiogenesis Apoptosis Differentiation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
Domain	Zinc-finger
Ligand	Magnesium Metal-binding Zinc
Molecular function	Developmental protein Endonuclease Hydrolase Nuclease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	angiogenesis Inferred from electronic annotation. Source: UniProtKB-KW apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: HPA nucleus Inferred from direct assay. Source: HPA plasma membrane Inferred from direct assay. Source: HPA
Molecular function	endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SMAD3	P84022	2	EBI-747793,EBI-347161

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 599	599	Ribonuclease ZC3H12A		PRO_0000341512
Regions
	Zinc finger	301 – 324	24	C3H1-type UniProtKB A6QQJ8
	Compositional bias	458 – 536	79	Pro-rich
Amino acid modifications
	Modified residue	386	1	Phosphoserine Ref.9
Natural variations
	Natural variant	240	1	V → M. Corresponds to variant rs16824179 [ dbSNP | Ensembl ].		VAR_052968
	Natural variant	547	1	G → D. Ref.2 Ref.3 Ref.6 Corresponds to variant rs17849897 [ dbSNP | Ensembl ].		VAR_044082
Experimental info
	Sequence conflict	248	1	D → G in CAG33645. Ref.3
	Sequence conflict	599	1	E → D in CAG33645. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q5D1E8 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 9213139FA7DCA443	FASTA	599	65,699
	10 20 30 40 50 60 MSGPCGEKPV LEASPTMSLW EFEDSHSRQG TPRPGQELAA EEASALELQM KVDFFRKLGY 70 80 90 100 110 120 SSTEIHSVLQ KLGVQADTNT VLGELVKHGT ATERERQTSP DPCPQLPLVP RGGGTPKAPN 130 140 150 160 170 180 LEPPLPEEEK EGSDLRPVVI DGSNVAMSHG NKEVFSCRGI LLAVNWFLER GHTDITVFVP 190 200 210 220 230 240 SWRKEQPRPD VPITDQHILR ELEKKKILVF TPSRRVGGKR VVCYDDRFIV KLAYESDGIV 250 260 270 280 290 300 VSNDTYRDLQ GERQEWKRFI EERLLMYSFV NDKFMPPDDP LGRHGPSLDN FLRKKPLTLE 310 320 330 340 350 360 HRKQPCPYGR KCTYGIKCRF FHPERPSCPQ RSVADELRAN ALLSPPRAPS KDKNGRRPSP 370 380 390 400 410 420 SSQSSSLLTE SEQCSLDGKK LGAQASPGSR QEGLTQTYAP SGRSLAPSGG SGSSFGPTDW 430 440 450 460 470 480 LPQTLDSLPY VSQDCLDSGI GSLESQMSEL WGVRGGGPGE PGPPRAPYTG YSPYGSELPA 490 500 510 520 530 540 TAAFSAFGRA MGAGHFSVPA DYPPAPPAFP PREYWSEPYP LPPPTSVLQE PPVQSPGAGR 550 560 570 580 590 SPWGRAGSLA KEQASVYTKL CGVFPPHLVE AVMGRFPQLL DPQQLAAEIL SYKSQHPSE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Monocyte chemoattractant protein-1 induces a novel transcription factor that causes cardiac myocyte apoptosis and ventricular dysfunction." Zhou L., Azfer A., Niu J., Graham S., Choudhury M., Adamski F.M., Younce C., Binkley P.F., Kolattukudy P.E. Circ. Res. 98:1177-1185(2006) [PubMed: 16574901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, POSSIBLE INVOLVEMENT IN ISCHEMIC HEART DISEASE.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASP-547. Tissue: Artery.
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASP-547.
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASP-547. Tissue: Kidney.
[7]	"A novel CCCH-zinc finger protein family regulates proinflammatory activation of macrophages." Liang J., Wang J., Azfer A., Song W., Tromp G., Kolattukudy P.E., Fu M. J. Biol. Chem. 283:6337-6346(2008) [PubMed: 18178554] [Abstract] Cited for: INDUCTION.
[8]	"Monocyte chemotactic protein (MCP)-1 promotes angiogenesis via a novel transcription factor, MCP-1-induced protein (MCPIP)." Niu J., Azfer A., Zhelyabovska O., Fatma S., Kolattukudy P.E. J. Biol. Chem. 283:14542-14551(2008) [PubMed: 18364357] [Abstract] Cited for: FUNCTION.
[9]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-386, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY920403 mRNA. Translation: AAX14017.1. AK026884 mRNA. Translation: BAB15581.1. CR457364 mRNA. Translation: CAG33645.1. AL034379, AL449284 Genomic DNA. Translation: CAI20551.1. AL449284, AL034379 Genomic DNA. Translation: CAH73689.1. CH471059 Genomic DNA. Translation: EAX07346.1. CH471059 Genomic DNA. Translation: EAX07347.1. BC005001 mRNA. Translation: AAH05001.1.
IPI	IPI00018002.
RefSeq	NP_079355.2. NM_025079.2.
UniGene	Hs.656294.
3D structure databases
ProteinModelPortal	Q5D1E8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5D1E8. 7 interactions.
STRING	Q5D1E8.
Polymorphism databases
DMDM	190479827.
Proteomic databases
PRIDE	Q5D1E8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000373082; ENSP00000362174; ENSG00000163874. ENST00000373087; ENSP00000362179; ENSG00000163874.
GeneID	80149.
KEGG	hsa:80149.
UCSC	uc001cbb.2. human.
Organism-specific databases
CTD	80149.
GeneCards	GC01P037940.
H-InvDB	HIX0000432.
HGNC	HGNC:26259. ZC3H12A.
HPA	HPA032052.
MIM	610562. gene.
neXtProt	NX_Q5D1E8.
PharmGKB	PA142670537.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG17279.
GeneTree	ENSGT00550000074448.
HOGENOM	HBG714846.
HOVERGEN	HBG108758.
InParanoid	Q5D1E8.
OMA	FPPREYW.
OrthoDB	EOG4Z62NZ.
PhylomeDB	Q5D1E8.
Gene expression databases
ArrayExpress	Q5D1E8.
Bgee	Q5D1E8.
CleanEx	HS_ZC3H12A.
Genevestigator	Q5D1E8.
Family and domain databases
InterPro	IPR021869. RNase_Zc3h12. [Graphical view]
Pfam	PF11977. RNase_Zc3h12a. 1 hit. [Graphical view]
PROSITE	PS50103. ZF_C3H1. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	70430.
SOURCE	Search...

Entry information

Entry name

ZC12A_HUMAN

Accession

Primary (citable) accession number: Q5D1E8
Secondary accession number(s): D3DPT0, Q6I9Z1, Q9H5P1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.