Reviewed, UniProtKB/Swiss-Prot Q5D189 (TYSY_BACNA)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45

Gene names

Name:

thyA

Organism

Bacillus subtilis subsp. natto

Taxonomic identifier

86029 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity. HAMAP MF_00008
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	Thymidylate synthase HAMAP MF_00008		PRO_0000140929
Sites
	Active site	161	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5D189-1 [UniParc]. Last modified March 29, 2005. Version 1. Checksum: 37D44AEA7057CFE7	FASTA	279	32,823
	10 20 30 40 50 60 MTQFDKQYNS IIKDIINNGI SDEEFDVRTK WDSDGTPAHT LSVMSKQMRF DNSEVPILTT 70 80 90 100 110 120 KKVAWKTAIK ELLWIWQLKS NDVNDLNKMG VHIWDQWKQE DGTIGHAYGF QLGKKNRSLN 130 140 150 160 170 180 GEKVDQVDYL LHQLKNNPSS RRHITMLWNP DDLDAMSLTP CVYETQWYVK HGKLHLEVRA 190 200 210 220 230 240 RSNDMALGNP FNVFQYNVLQ RMIAQVTGYE LGEYIFNIGD CHVYTRHIDN LKIQMEREQF 250 260 270 EAPELWINPE VKDFYNFTID DFKLINYKHG DKLLFEVAV

References

[1]	Qin Z.H., Cai J.P., Ye X.H., Wang X., Wang P.Y., Chen W., Xie M.Q. Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: KX.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY920747 Genomic DNA. Translation: AAX18246.1.
3D structure databases
SMR	Q5D189. Positions 1-279.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.1.1.45. 298357.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_BACNA

Accession

Primary (citable) accession number: Q5D189

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	March 29, 2005
Last modified:	February 9, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)