ID   Q5D078_MOUSE            Unreviewed;       205 AA.
AC   Q5D078;
DT   10-MAY-2005, integrated into UniProtKB/TrEMBL.
DT   10-MAY-2005, sequence version 1.
DT   27-MAR-2024, entry version 144.
DE   RecName: Full=Regulator of G-protein signaling 4 {ECO:0000256|ARBA:ARBA00020122};
GN   Name=Rgs4 {ECO:0000313|EMBL:ABC94587.1,
GN   ECO:0000313|Ensembl:ENSMUSP00000027991.6, ECO:0000313|MGI:MGI:108409};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090 {ECO:0000313|EMBL:AAH55293.1};
RN   [1] {ECO:0000313|EMBL:BAC27009.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAC27009.1};
RC   TISSUE=Hypothalamus {ECO:0000313|EMBL:BAC30249.1}, and Pituitary gland
RC   {ECO:0000313|EMBL:BAC27009.1};
RX   PubMed=10349636; DOI=10.1016/S0076-6879(99)03004-9;
RA   Carninci P., Hayashizaki Y.;
RT   "High-efficiency full-length cDNA cloning.";
RL   Methods Enzymol. 303:19-44(1999).
RN   [2] {ECO:0000313|EMBL:BAC27009.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAC27009.1};
RC   TISSUE=Hypothalamus {ECO:0000313|EMBL:BAC30249.1}, and Pituitary gland
RC   {ECO:0000313|EMBL:BAC27009.1};
RX   PubMed=11042159; DOI=10.1101/gr.145100;
RA   Carninci P., Shibata Y., Hayatsu N., Sugahara Y., Shibata K., Itoh M.,
RA   Konno H., Okazaki Y., Muramatsu M., Hayashizaki Y.;
RT   "Normalization and subtraction of cap-trapper-selected cDNAs to prepare
RT   full-length cDNA libraries for rapid discovery of new genes.";
RL   Genome Res. 10:1617-1630(2000).
RN   [3] {ECO:0000313|EMBL:BAC27009.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAC27009.1};
RC   TISSUE=Hypothalamus {ECO:0000313|EMBL:BAC30249.1}, and Pituitary gland
RC   {ECO:0000313|EMBL:BAC27009.1};
RX   PubMed=11076861; DOI=10.1101/gr.152600;
RA   Shibata K., Itoh M., Aizawa K., Nagaoka S., Sasaki N., Carninci P.,
RA   Konno H., Akiyama J., Nishi K., Kitsunai T., Tashiro H., Itoh M., Sumi N.,
RA   Ishii Y., Nakamura S., Hazama M., Nishine T., Harada A., Yamamoto R.,
RA   Matsumoto H., Sakaguchi S., Ikegami T., Kashiwagi K., Fujiwake S.,
RA   Inoue K., Togawa Y., Izawa M., Ohara E., Watahiki M., Yoneda Y.,
RA   Ishikawa T., Ozawa K., Tanaka T., Matsuura S., Kawai J., Okazaki Y.,
RA   Muramatsu M., Inoue Y., Kira A., Hayashizaki Y.;
RT   "RIKEN integrated sequence analysis (RISA) system--384-format sequencing
RT   pipeline with 384 multicapillary sequencer.";
RL   Genome Res. 10:1757-1771(2000).
RN   [4] {ECO:0000313|EMBL:BAC27009.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAC27009.1};
RC   TISSUE=Hypothalamus {ECO:0000313|EMBL:BAC30249.1}, and Pituitary gland
RC   {ECO:0000313|EMBL:BAC27009.1};
RX   PubMed=11217851; DOI=10.1038/35055500;
RG   The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium;
RT   "Functional annotation of a full-length mouse cDNA collection.";
RL   Nature 409:685-690(2001).
RN   [5] {ECO:0000313|EMBL:BAC27009.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAC27009.1};
RC   TISSUE=Hypothalamus {ECO:0000313|EMBL:BAC30249.1}, and Pituitary gland
RC   {ECO:0000313|EMBL:BAC27009.1};
RA   Adachi J., Aizawa K., Akimura T., Arakawa T., Bono H., Carninci P.,
RA   Fukuda S., Furuno M., Hanagaki T., Hara A., Hashizume W., Hayashida K.,
RA   Hayatsu N., Hiramoto K., Hiraoka T., Hirozane T., Hori F., Imotani K.,
RA   Ishii Y., Itoh M., Kagawa I., Kasukawa T., Katoh H., Kawai J., Kojima Y.,
RA   Kondo S., Konno H., Kouda M., Koya S., Kurihara C., Matsuyama T.,
RA   Miyazaki A., Murata M., Nakamura M., Nishi K., Nomura K., Numazaki R.,
RA   Ohno M., Ohsato N., Okazaki Y., Saito R., Saitoh H., Sakai C., Sakai K.,
RA   Sakazume N., Sano H., Sasaki D., Shibata K., Shinagawa A., Shiraki T.,
RA   Sogabe Y., Tagami M., Tagawa A., Takahashi F., Takaku-Akahira S.,
RA   Takeda Y., Tanaka T., Tomaru A., Toya T., Yasunishi A., Muramatsu M.,
RA   Hayashizaki Y.;
RL   Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN   [6] {ECO:0000313|EMBL:BAC27009.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAC27009.1};
RC   TISSUE=Hypothalamus {ECO:0000313|EMBL:BAC30249.1}, and Pituitary gland
RC   {ECO:0000313|EMBL:BAC27009.1};
RX   PubMed=12466851; DOI=10.1038/nature01266;
RG   The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I and II Team;
RT   "Analysis of the mouse transcriptome based on functional annotation of
RT   60,770 full-length cDNAs.";
RL   Nature 420:563-573(2002).
RN   [7] {ECO:0000313|EMBL:AAH55293.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Eye {ECO:0000313|EMBL:AAH55293.1};
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RA   Gerhard D.S., Wagner L., Feingold E.A., Shenmen C.M., Grouse L.H.,
RA   Schuler G., Klein S.L., Old S., Rasooly R., Good P., Guyer M., Peck A.M.,
RA   Derge J.G., Lipman D., Collins F.S., Jang W., Sherry S., Feolo M.,
RA   Misquitta L., Lee E., Rotmistrovsky K., Greenhut S.F., Schaefer C.F.,
RA   Buetow K., Bonner T.I., Haussler D., Kent J., Kiekhaus M., Furey T.,
RA   Brent M., Prange C., Schreiber K., Shapiro N., Bhat N.K., Hopkins R.F.,
RA   Hsie F., Driscoll T., Soares M.B., Casavant T.L., Scheetz T.E.,
RA   Brown-stein M.J., Usdin T.B., Toshiyuki S., Carninci P., Piao Y.,
RA   Dudekula D.B., Ko M.S., Kawakami K., Suzuki Y., Sugano S., Gruber C.E.,
RA   Smith M.R., Simmons B., Moore T., Waterman R., Johnson S.L., Ruan Y.,
RA   Wei C.L., Mathavan S., Gunaratne P.H., Wu J., Garcia A.M., Hulyk S.W.,
RA   Fuh E., Yuan Y., Sneed A., Kowis C., Hodgson A., Muzny D.M., McPherson J.,
RA   Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S.,
RA   Sanchez A., Whiting M., Madari A., Young A.C., Wetherby K.D., Granite S.J.,
RA   Kwong P.N., Brinkley C.P., Pearson R.L., Bouffard G.G., Blakesly R.W.,
RA   Green E.D., Dickson M.C., Rodriguez A.C., Grimwood J., Schmutz J.,
RA   Myers R.M., Butterfield Y.S., Griffith M., Griffith O.L., Krzywinski M.I.,
RA   Liao N., Morin R., Morrin R., Palmquist D., Petrescu A.S., Skalska U.,
RA   Smailus D.E., Stott J.M., Schnerch A., Schein J.E., Jones S.J., Holt R.A.,
RA   Baross A., Marra M.A., Clifton S., Makowski K.A., Bosak S., Malek J.;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [8] {ECO:0000313|EMBL:BAC27009.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAC27009.1};
RC   TISSUE=Hypothalamus {ECO:0000313|EMBL:BAC30249.1}, and Pituitary gland
RC   {ECO:0000313|EMBL:BAC27009.1};
RG   The FANTOM Consortium;
RG   Riken Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group);
RT   "The Transcriptional Landscape of the Mammalian Genome.";
RL   Science 309:1559-1563(2005).
RN   [9] {ECO:0000313|EMBL:BAC27009.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAC27009.1};
RC   TISSUE=Hypothalamus {ECO:0000313|EMBL:BAC30249.1}, and Pituitary gland
RC   {ECO:0000313|EMBL:BAC27009.1};
RX   PubMed=16141073; DOI=10.1126/science.1112009;
RG   RIKEN Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group) and the FANTOM Consortium;
RT   "Antisense Transcription in the Mammalian Transcriptome.";
RL   Science 309:1564-1566(2005).
RN   [10] {ECO:0000313|EMBL:ABC94587.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6 {ECO:0000313|EMBL:ABC94587.1};
RX   PubMed=17707117; DOI=10.1016/j.gene.2007.07.002;
RA   Ding L., Mychaleckyj J.C., Hegde A.N.;
RT   "Full length cloning and expression analysis of splice variants of
RT   regulator of G-protein signaling RGS4 in human and murine brain.";
RL   Gene 401:46-60(2007).
RN   [11] {ECO:0000313|Ensembl:ENSMUSP00000027991.6, ECO:0000313|Proteomes:UP000000589}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000027991.6,
RC   ECO:0000313|Proteomes:UP000000589};
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [12] {ECO:0000313|Ensembl:ENSMUSP00000027991.6}
RP   IDENTIFICATION.
RC   STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000027991.6};
RG   Ensembl;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- FUNCTION: Inhibits signal transduction by increasing the GTPase
CC       activity of G protein alpha subunits thereby driving them into their
CC       inactive GDP-bound form. Activity on G(z)-alpha is inhibited by
CC       phosphorylation of the G-protein. Activity on G(z)-alpha and G(i)-
CC       alpha-1 is inhibited by palmitoylation of the G-protein.
CC       {ECO:0000256|ARBA:ARBA00025480}.
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DR   EMBL; BC055293; AAH55293.1; -; mRNA.
DR   EMBL; DQ346659; ABC94587.1; -; mRNA.
DR   EMBL; DQ346660; ABC94588.1; -; mRNA.
DR   EMBL; DQ346661; ABC94589.1; -; mRNA.
DR   EMBL; AK030536; BAC27009.1; -; mRNA.
DR   EMBL; AK039134; BAC30249.1; -; mRNA.
DR   RefSeq; NP_033088.2; NM_009062.3.
DR   ProteomicsDB; 341325; -.
DR   Antibodypedia; 20521; 308 antibodies from 33 providers.
DR   DNASU; 19736; -.
DR   Ensembl; ENSMUST00000027991.12; ENSMUSP00000027991.6; ENSMUSG00000038530.12.
DR   GeneID; 19736; -.
DR   KEGG; mmu:19736; -.
DR   UCSC; uc007dln.1; mouse.
DR   AGR; MGI:108409; -.
DR   CTD; 5999; -.
DR   MGI; MGI:108409; Rgs4.
DR   VEuPathDB; HostDB:ENSMUSG00000038530; -.
DR   GeneTree; ENSGT00940000159036; -.
DR   HOGENOM; CLU_059863_3_0_1; -.
DR   OMA; LIQDLCQ; -.
DR   OrthoDB; 22856at2759; -.
DR   TreeFam; TF315837; -.
DR   BioGRID-ORCS; 19736; 2 hits in 76 CRISPR screens.
DR   ChiTaRS; Rgs4; mouse.
DR   Proteomes; UP000000589; Chromosome 1.
DR   Bgee; ENSMUSG00000038530; Expressed in superior cervical ganglion and 203 other cell types or tissues.
DR   GO; GO:0005737; C:cytoplasm; IEA:Ensembl.
DR   GO; GO:0005634; C:nucleus; IEA:Ensembl.
DR   GO; GO:0032991; C:protein-containing complex; IEA:Ensembl.
DR   GO; GO:0001965; F:G-protein alpha-subunit binding; IEA:Ensembl.
DR   GO; GO:0005096; F:GTPase activator activity; IEA:Ensembl.
DR   GO; GO:0019901; F:protein kinase binding; IEA:Ensembl.
DR   GO; GO:1990791; P:dorsal root ganglion development; IEA:Ensembl.
DR   GO; GO:0061052; P:negative regulation of cell growth involved in cardiac muscle cell development; IEA:Ensembl.
DR   GO; GO:0060160; P:negative regulation of dopamine receptor signaling pathway; IEA:Ensembl.
DR   GO; GO:0045744; P:negative regulation of G protein-coupled receptor signaling pathway; IEA:Ensembl.
DR   GO; GO:1900924; P:negative regulation of glycine import across plasma membrane; IEA:Ensembl.
DR   GO; GO:1901380; P:negative regulation of potassium ion transmembrane transport; IEA:Ensembl.
DR   GO; GO:2000463; P:positive regulation of excitatory postsynaptic potential; IEA:Ensembl.
DR   GO; GO:0010460; P:positive regulation of heart rate; IEA:Ensembl.
DR   GO; GO:0110053; P:regulation of actin filament organization; IEA:Ensembl.
DR   GO; GO:0051924; P:regulation of calcium ion transport; IEA:Ensembl.
DR   GO; GO:0001975; P:response to amphetamine; IEA:Ensembl.
DR   GO; GO:0042220; P:response to cocaine; IEA:Ensembl.
DR   GO; GO:0045471; P:response to ethanol; IEA:Ensembl.
DR   CDD; cd08714; RGS_RGS4; 1.
DR   Gene3D; 1.10.196.10; -; 1.
DR   Gene3D; 1.10.167.10; Regulator of G-protein Signalling 4, domain 2; 1.
DR   InterPro; IPR016137; RGS.
DR   InterPro; IPR034953; RGS_RGS4.
DR   InterPro; IPR036305; RGS_sf.
DR   InterPro; IPR024066; RGS_subdom1/3.
DR   InterPro; IPR044926; RGS_subdomain_2.
DR   PANTHER; PTHR10845; REGULATOR OF G PROTEIN SIGNALING; 1.
DR   PANTHER; PTHR10845:SF184; REGULATOR OF G-PROTEIN SIGNALING 4; 1.
DR   Pfam; PF00615; RGS; 1.
DR   PRINTS; PR01301; RGSPROTEIN.
DR   SMART; SM00315; RGS; 1.
DR   SUPFAM; SSF48097; Regulator of G-protein signaling, RGS; 1.
DR   PROSITE; PS50132; RGS; 1.
PE   2: Evidence at transcript level;
KW   Lipoprotein {ECO:0000256|ARBA:ARBA00023288};
KW   Palmitate {ECO:0000256|ARBA:ARBA00023139};
KW   Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000589};
KW   Signal transduction inhibitor {ECO:0000256|ARBA:ARBA00022700}.
FT   DOMAIN          62..178
FT                   /note="RGS"
FT                   /evidence="ECO:0000259|PROSITE:PS50132"
SQ   SEQUENCE   205 AA;  23261 MW;  5D795809DE9D1B7C CRC64;
     MCKGLAGLPA SCLRSAKDMK HRLGFLLQKS DSCEHSSSHS KKDKVVTCQR VSQEEVKKWA
     ESLENLIHHE CGLAAFKAFL KSEYSEENID FWISCEEYKK IKSPSKLSPK AKKIYNEFIS
     VQATKEVNLD SCTREETSRN MLQPTITCFD EAQKKIFNLM EKDSYRRFLK SRFYLDLTNP
     SSCGAEKQKG AKSSADCTSL VSQCA
//