Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyruvate kinase

Gene

cgd1_2040

Organism
Cryptosporidium parvum (strain Iowa II)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (cgd6_3790)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Pyruvate kinase (cgd1_2040)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, PyruvateImported

Enzyme and pathway databases

BRENDAi2.7.1.40. 1728.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinaseUniRule annotation (EC:2.7.1.40UniRule annotation)
Gene namesi
ORF Names:cgd1_2040Imported
OrganismiCryptosporidium parvum (strain Iowa II)Imported
Taxonomic identifieri353152 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaConoidasidaCoccidiaEucoccidioridaEimeriorinaCryptosporidiidaeCryptosporidium
Proteomesi
  • UP000006726 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiCryptoDB:cgd1_2040.

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi32 – 32Interchain (with C-318)Combined sources
Disulfide bondi318 – 318Interchain (with C-32)Combined sources

Interactioni

Protein-protein interaction databases

STRINGi353152.XP_628040.1.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MA8X-ray2.64A/B17-532[»]
4DRSX-ray2.50A/B7-532[»]
ProteinModelPortaliQ5CSM7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5CSM7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini50 – 397348PKInterPro annotationAdd
BLAST
Domaini416 – 529114PK_CInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the pyruvate kinase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2323. Eukaryota.
COG0469. LUCA.
HOGENOMiHOG000021559.
InParanoidiQ5CSM7.
KOiK00873.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Q5CSM7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
IKKFTKMISN DHLKRLASTS AVMSCTLGKA TCLGMDKICS PLADNDVTQR
60 70 80 90 100
KTQIICTIGP SCNNVESLIG LIDKGMSVAR LNFSHGDHES HFKTLQNIRE
110 120 130 140 150
AAKARPHSTV GIMLDTKGPE IRTGMLEGGK PIELKAGQTL KITTDYSMLG
160 170 180 190 200
NSECISCSYS LLPKSVQIGS TVLIADGSLS TQVLEIGDDF IVCKVLNSVT
210 220 230 240 250
IGERKNMNLP GCKVHLPIIG DKDRHDIVDF ALKYNLDFIA LSFVQNGADV
260 270 280 290 300
QLCRQIISEN TQYSNGIPSS IKIISKIENL EGVINFDSIC SESDGIMVAR
310 320 330 340 350
GDLGMEIPPE KIFVAQKCMI SKCNVAGKPV VTATQMLESM IKSNRPTRAE
360 370 380 390 400
MTDVANAVLD GSDCVMLSGE TANGAFPFDA VNVMSRVCAQ AETCIDYPVL
410 420 430 440 450
YHAIHSSVPK PVAVPEAIAC SAVESAHDVN AKLIITITET GNTARLISKY
460 470 480 490 500
RPSQTIIACT AKPEVARGLK IARGVKTYVL NSIHHSEVVI SNALALAKEE
510 520 530
SLIESGDFAI AVHGVKESCP GSCNLMKIVR CP
Length:532
Mass (Da):57,172
Last modified:April 12, 2005 - v1
Checksum:iF2950B843B61A41A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11Imported

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAEE01000006 Genomic DNA. Translation: EAK88569.1.
RefSeqiXP_628040.1. XM_628040.1.

Genome annotation databases

EnsemblProtistsiEAK88569; EAK88569; cgd1_2040.
GeneIDi3371357.
KEGGicpv:cgd1_2040.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAEE01000006 Genomic DNA. Translation: EAK88569.1.
RefSeqiXP_628040.1. XM_628040.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MA8X-ray2.64A/B17-532[»]
4DRSX-ray2.50A/B7-532[»]
ProteinModelPortaliQ5CSM7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi353152.XP_628040.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAK88569; EAK88569; cgd1_2040.
GeneIDi3371357.
KEGGicpv:cgd1_2040.

Organism-specific databases

EuPathDBiCryptoDB:cgd1_2040.

Phylogenomic databases

eggNOGiKOG2323. Eukaryota.
COG0469. LUCA.
HOGENOMiHOG000021559.
InParanoidiQ5CSM7.
KOiK00873.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.
BRENDAi2.7.1.40. 1728.

Miscellaneous databases

EvolutionaryTraceiQ5CSM7.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Iowa IIImported.
  2. "Crystal structure of CGD1_2040, a pyruvate kinase from cryptosporidium Parvum."
    Wernimont A.K., Hutchinson A., Hassanali A., Kozieradzki I., Cossar D., Bochkarev A., Arrowsmith C.H., Edwards A.M., Bountra C., Weigelt J., Hui R., Hills T., Pizarro J.C.
    Submitted (MAR-2010) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.64 ANGSTROMS) OF 17-532.
  3. "Crystal structure of Cryptosporidium parvum pyruvate kinase."
    Cook W.J., Senkovich O., Aleem K., Chattopadhyay D.
    PLoS ONE 7:e46875-e46875(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 7-532, DISULFIDE BONDS.

Entry informationi

Entry nameiQ5CSM7_CRYPI
AccessioniPrimary (citable) accession number: Q5CSM7
Entry historyi
Integrated into UniProtKB/TrEMBL: April 12, 2005
Last sequence update: April 12, 2005
Last modified: June 8, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.