Reviewed, UniProtKB/Swiss-Prot Q5CB04 (B3GA1_PANTR)

Names and origin

Protein names

Recommended name:
    Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
    EC=2.4.1.135
Alternative name(s):
    Beta-1,3-glucuronyltransferase 1
    Glucuronosyltransferase-P
      Short name=GlcAT-P
    UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase
      Short name=GlcUAT-P

Gene names

Name:

B3GAT1

Organism

Pan troglodytes (Chimpanzee)

Taxonomic identifier

9598 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pan

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group By similarity.
Catalytic activity	UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Homodimer Potential.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 43 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Manganese Metal-binding
Molecular function	Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1		PRO_0000195169
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Signal-anchor for type II membrane protein Potential
	Topological domain	28 – 332	305	Lumenal Potential
Sites
	Active site	282	1	Proton acceptor By similarity
	Metal binding	197	1	Manganese By similarity
Amino acid modifications
	Glycosylation	140	1	N-linked (GlcNAc...) Potential
	Glycosylation	184	1	N-linked (GlcNAc...) Potential
	Glycosylation	301	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5CB04-1 [UniParc]. Last modified April 12, 2005. Version 1. Checksum: 6ECBA46DB961D149	FASTA	332	38,005
	10 20 30 40 50 60 MPKRRDILAI VLIVLPWTLL ITVWHQSTLA PLLAVHKDEG SDPRRETPPG ADPREYCMSD 70 80 90 100 110 120 RDIVEVVRTE YVYTRPPPWS DTLPTIHVVT PTYSRPVQKA ELTRMANTLL HVPNLHWLVV 130 140 150 160 170 180 EDAPRRTPLT ARLLRDTGLN YTHLHVETPR NYKLRGDARD PRIPRGTMQR NLALRWLRET 190 200 210 220 230 240 FPRNSSQPGV VYFADDDNPY SLELFQKVTR RVSVWPVAFV GGLRYEAPRV NGAGKVVGWK 250 260 270 280 290 300 TVFDPHRPFA IDMAGFAVNL RLILQRSQAY FKLRGVKGGY QESSLLRELV TLNDLEPKAA 310 320 330 NCTKILVWHT RTEKPVLVNE GKKGFTDPSV EI

References

[1]	"Phylogeny of beta3-glucuronyltransferases." Ouzzine M., Magdalou J., Fournel-Gigleux S., Mollicone R., Oriol R. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ888974 mRNA. Translation: CAI62039.1.
RefSeq	NP_001026790.1.
3D structure databases
SMR	Q5CB04. Positions 83-332.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5CB04.
Protein family/group databases
CAZy	GT43. Glycosyltransferase Family 43.
Genome annotation databases
Ensembl	ENSPTRT00000061611; ENSPTRP00000054150; ENSPTRG00000004501; Pan troglodytes. [Genome view] ENSPTRT00000061613; ENSPTRP00000054152; ENSPTRG00000004501; Pan troglodytes. [Genome view]
GeneID	466859.
KEGG	ptr:466859.
Organism-specific databases
CTD	466859.
Phylogenomic databases
eggNOG	prNOG12174.
HOVERGEN	Q5CB04.
Enzyme and pathway databases
BRENDA	2.4.1.135. 264977.
Family and domain databases
InterPro	IPR005027. Glyco_trans_43. [Graphical view]
PANTHER	PTHR10896. Glyco_trans_43. 1 hit.
Pfam	PF03360. Glyco_transf_43. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B3GA1_PANTR

Accession

Primary (citable) accession number: Q5CB04

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	April 12, 2005
Last modified:	January 19, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)