Q5CB03 (B3GA1_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
EC=2.4.1.135

Alternative name(s):
Beta-1,3-glucuronyltransferase 1
Glucuronosyltransferase P
Short name=GlcAT-P
UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase
Short name=GlcUAT-P

Gene names

Name:

B3GAT1

Organism

Canis familiaris (Dog) (Canis lupus familiaris)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group By similarity.
Catalytic activity	UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Homodimer Potential.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 43 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese Metal-binding
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1		PRO_0000195166
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	28 – 335	308	Lumenal Potential
Sites
	Active site	285	1	Proton acceptor By similarity
	Metal binding	198	1	Manganese By similarity
Amino acid modifications
	Glycosylation	141	1	N-linked (GlcNAc...) Potential
	Glycosylation	185	1	N-linked (GlcNAc...) Potential
	Glycosylation	304	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5CB03 [UniParc]. Last modified April 12, 2005. Version 1. Checksum: 194B8FE05DBE4864	FASTA	335	38,361
	10 20 30 40 50 60 MPKRRDILAI VLIVLPWTLL VTVWHQSTIA PLLTTHKGEP LTDSRREAAP GADPREYCMS 70 80 90 100 110 120 DRDIVEVVRT EYVYTRPPPW SDTLPTIHVV TPTYSRPVQK AELTRMANTL LHVPNLHWLV 130 140 150 160 170 180 VEDAPRRTPL TARLLRDTGL NYTHLHVETP RNYKLRGDAR DPRIPRGTMQ RNLALRWLRE 190 200 210 220 230 240 TFPRNSSQPG VVYFADDDNT YSLELFEEMR STRRVSVWPV AFVGGLRYEA PRVNGAGKVV 250 260 270 280 290 300 GWKTVFDPHR PFAIDMAGFA VNLRLILQRS QAYFKLRGVK GGYQESSLLR ELVTLNDLEP 310 320 330 KAANCTKILV WHTRTEKPVL VNEGKKGFTD PTVEI

References

[1]	"Phylogeny of beta3-glucuronyltransferases." Ouzzine M., Magdalou J., Fournel-Gigleux S., Mollicone R., Oriol R. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ888975 mRNA. Translation: CAI62040.1.
RefSeq	NP_001026804.1. NM_001031634.1.
UniGene	Cfa.16342.
3D structure databases
ProteinModelPortal	Q5CB03.
SMR	Q5CB03. Positions 84-335.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5CB03.
Protein family/group databases
CAZy	GT43. Glycosyltransferase Family 43.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	489265.
KEGG	cfa:489265.
Organism-specific databases
CTD	27087.
Phylogenomic databases
eggNOG	maNOG04873.
HOVERGEN	HBG050650.
InParanoid	Q5CB03.
Family and domain databases
InterPro	IPR005027. Glyco_trans_43. [Graphical view]
KO	K00735.
PANTHER	PTHR10896. Glyco_trans_43. 1 hit.
Pfam	PF03360. Glyco_transf_43. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B3GA1_CANFA

Accession

Primary (citable) accession number: Q5CB03

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	April 12, 2005
Last modified:	November 16, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program