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Protein

Venom acid phosphatase Acph-1

Gene
N/A
Organism
Apis mellifera (Honeybee)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei26 – 261NucleophileBy similarity
Active sitei273 – 2731Proton donorBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BRENDAi3.1.3.2. 387.

Names & Taxonomyi

Protein namesi
Recommended name:
Venom acid phosphatase Acph-1 (EC:3.1.3.2)
Alternative name(s):
Allergen: Api m 3
OrganismiApis mellifera (Honeybee)
Taxonomic identifieri7460 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataApoideaApidaeApis
ProteomesiUP000005203 Componentsi: Unassembled WGS sequence, Unplaced

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.2 Publications

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei2778. Api m A1-A2-A3.
3090. Api m 3.0101.
47. Api m 3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1515Sequence AnalysisAdd
BLAST
Chaini16 – 388373Venom acid phosphatase Acph-1PRO_5000095341Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi145 ↔ 355By similarity
Glycosylationi182 – 1821N-linked (GlcNAc...)Sequence Analysis
Glycosylationi228 – 2281N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi330 ↔ 334By similarity
Glycosylationi366 – 3661N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ5BLY5.

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

3D structure databases

ProteinModelPortaliQ5BLY5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the histidine acid phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG291245.
InParanoidiQ5BLY5.
OMAiIPEWAKP.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
InterProiIPR029630. Api_m_3.
IPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view]
PANTHERiPTHR11567:SF22. PTHR11567:SF22. 1 hit.
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5BLY5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVIAILAMV VGVQAELKQI NVIFRHGDRI PDEKNEMYPK DPYLYYDFYP
60 70 80 90 100
LERGELTNSG KMREYQLGQF LRERYGDFLG DIYTEESVSA LSSFYDRTKM
110 120 130 140 150
SLQLVLAALY PPNKLQQWNE DLNWQPIATK YLRRYEDNIF LPEDCLLFTI
160 170 180 190 200
ELDRVLESPR GKYEFSKYDK LKKKLEEWTG KNITTPWDYY YIYHTLVAEQ
210 220 230 240 250
SYGLTLPSWT NNIFPRGELF DATVFTYNIT NSTPLLKKLY GGPLLRIFTK
260 270 280 290 300
HMLDVVSGTQ KKKRKIYLFS GHESNIASVL HALQLYYPHV PEYSSSIIME
310 320 330 340 350
LHNIEGTHYV KIVYYLGIPS EARELQLPGC EVLCPLYKYL QLIENVIPSN
360 370 380
EELICDKRFV DESANNLSIE ELDFVKLNLI RIAGTENK
Length:388
Mass (Da):45,389
Last modified:April 11, 2005 - v1
Checksum:iDD3327ECC2B03C6B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti152 – 1521L → F in ACI25605 (Ref. 2) Curated
Sequence conflicti278 – 2781S → A in ACI25605 (Ref. 2) Curated
Sequence conflicti323 – 3231R → G in ACI25605 (Ref. 2) Curated
Sequence conflicti360 – 3601V → A in ACI25605 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY939855 mRNA. Translation: AAX33235.1.
FJ200211 mRNA. Translation: ACI25605.1.
DQ058012 mRNA. Translation: AAY57281.1.
UniGeneiAme.7483.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY939855 mRNA. Translation: AAX33235.1.
FJ200211 mRNA. Translation: ACI25605.1.
DQ058012 mRNA. Translation: AAY57281.1.
UniGeneiAme.7483.

3D structure databases

ProteinModelPortaliQ5BLY5.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei2778. Api m A1-A2-A3.
3090. Api m 3.0101.
47. Api m 3.

Proteomic databases

PaxDbiQ5BLY5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiNOG291245.
InParanoidiQ5BLY5.
OMAiIPEWAKP.

Enzyme and pathway databases

BRENDAi3.1.3.2. 387.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
InterProiIPR029630. Api_m_3.
IPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view]
PANTHERiPTHR11567:SF22. PTHR11567:SF22. 1 hit.
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and characterization of honeybee venom acid phosphatase."
    Hoffman D.R., Weimer E.T., Sakell R.H., Schmidt M.
    J. Allergy Clin. Immunol. 115:S107-S107(2004)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 19-53; 62-72; 75-130; 134-154 AND 312-381, IDENTIFICATION BY MASS SPECTROMETRY, ALLERGEN.
    Tissue: Venom and Venom gland.
  2. "Molecular cloning of honeybee venom allergen acid phosphatase."
    Zhang Q., Liu Z., Wu Y.
    Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Venom gland.
  3. "Insights into social insects from the genome of the honeybee Apis mellifera."
    Honeybee genome sequencing consortium
    Nature 443:931-949(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Molecular cloning and expression in insect cells of honeybee venom allergen acid phosphatase (Api m 3)."
    Grunwald T., Bockisch B., Spillner E., Ring J., Bredehorst R., Ollert M.W.
    J. Allergy Clin. Immunol. 117:848-854(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-388, ALLERGEN.
    Tissue: Venom gland.
  5. Cited for: REVIEW.

Entry informationi

Entry nameiACPH1_APIME
AccessioniPrimary (citable) accession number: Q5BLY5
Secondary accession number(s): B6E2X9, Q4TUB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 29, 2010
Last sequence update: April 11, 2005
Last modified: March 31, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.