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Q5BLY5

- ACPH1_APIME

UniProt

Q5BLY5 - ACPH1_APIME

Protein

Venom acid phosphatase Acph-1

Gene
N/A
Organism
Apis mellifera (Honeybee)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (12 Apr 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei26 – 261NucleophileBy similarity
    Active sitei273 – 2731Proton donorBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Venom acid phosphatase Acph-1 (EC:3.1.3.2)
    Alternative name(s):
    Allergen: Api m 3
    OrganismiApis mellifera (Honeybee)
    Taxonomic identifieri7460 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataApoideaApidaeApis
    ProteomesiUP000005203: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Allergenic propertiesi

    Causes an allergic reaction in human.2 Publications

    Keywords - Diseasei

    Allergen

    Protein family/group databases

    Allergomei2778. Api m A1-A2-A3.
    3090. Api m 3.0101.
    47. Api m 3.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1515Sequence AnalysisAdd
    BLAST
    Chaini16 – 388373Venom acid phosphatase Acph-1PRO_5000095341Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi145 ↔ 355By similarity
    Glycosylationi182 – 1821N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi228 – 2281N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi330 ↔ 334By similarity
    Glycosylationi366 – 3661N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ5BLY5.

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5BLY5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the histidine acid phosphatase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG291245.
    InParanoidiQ5BLY5.
    OMAiGHENNIA.

    Family and domain databases

    Gene3Di3.40.50.1240. 1 hit.
    InterProiIPR029630. Api_m_3.
    IPR000560. His_Pase_superF_clade-2.
    IPR029033. His_PPase_superfam.
    [Graphical view]
    PANTHERiPTHR11567:SF22. PTHR11567:SF22. 1 hit.
    PfamiPF00328. His_Phos_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53254. SSF53254. 1 hit.
    PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5BLY5-1 [UniParc]FASTAAdd to Basket

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    MSVIAILAMV VGVQAELKQI NVIFRHGDRI PDEKNEMYPK DPYLYYDFYP    50
    LERGELTNSG KMREYQLGQF LRERYGDFLG DIYTEESVSA LSSFYDRTKM 100
    SLQLVLAALY PPNKLQQWNE DLNWQPIATK YLRRYEDNIF LPEDCLLFTI 150
    ELDRVLESPR GKYEFSKYDK LKKKLEEWTG KNITTPWDYY YIYHTLVAEQ 200
    SYGLTLPSWT NNIFPRGELF DATVFTYNIT NSTPLLKKLY GGPLLRIFTK 250
    HMLDVVSGTQ KKKRKIYLFS GHESNIASVL HALQLYYPHV PEYSSSIIME 300
    LHNIEGTHYV KIVYYLGIPS EARELQLPGC EVLCPLYKYL QLIENVIPSN 350
    EELICDKRFV DESANNLSIE ELDFVKLNLI RIAGTENK 388
    Length:388
    Mass (Da):45,389
    Last modified:April 12, 2005 - v1
    Checksum:iDD3327ECC2B03C6B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti152 – 1521L → F in ACI25605. 1 PublicationCurated
    Sequence conflicti278 – 2781S → A in ACI25605. 1 PublicationCurated
    Sequence conflicti323 – 3231R → G in ACI25605. 1 PublicationCurated
    Sequence conflicti360 – 3601V → A in ACI25605. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY939855 mRNA. Translation: AAX33235.1.
    FJ200211 mRNA. Translation: ACI25605.1.
    DQ058012 mRNA. Translation: AAY57281.1.
    RefSeqiNP_001013377.2. NM_001013359.2.
    UniGeneiAme.7483.

    Genome annotation databases

    EnsemblMetazoaiGB12546-RA; GB12546-PA; GB12546.
    GeneIDi411830.
    KEGGiame:411830.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY939855 mRNA. Translation: AAX33235.1 .
    FJ200211 mRNA. Translation: ACI25605.1 .
    DQ058012 mRNA. Translation: AAY57281.1 .
    RefSeqi NP_001013377.2. NM_001013359.2.
    UniGenei Ame.7483.

    3D structure databases

    ProteinModelPortali Q5BLY5.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 2778. Api m A1-A2-A3.
    3090. Api m 3.0101.
    47. Api m 3.

    Proteomic databases

    PaxDbi Q5BLY5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai GB12546-RA ; GB12546-PA ; GB12546 .
    GeneIDi 411830.
    KEGGi ame:411830.

    Organism-specific databases

    CTDi 48445.

    Phylogenomic databases

    eggNOGi NOG291245.
    InParanoidi Q5BLY5.
    OMAi GHENNIA.

    Family and domain databases

    Gene3Di 3.40.50.1240. 1 hit.
    InterProi IPR029630. Api_m_3.
    IPR000560. His_Pase_superF_clade-2.
    IPR029033. His_PPase_superfam.
    [Graphical view ]
    PANTHERi PTHR11567:SF22. PTHR11567:SF22. 1 hit.
    Pfami PF00328. His_Phos_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53254. SSF53254. 1 hit.
    PROSITEi PS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and characterization of honeybee venom acid phosphatase."
      Hoffman D.R., Weimer E.T., Sakell R.H., Schmidt M.
      J. Allergy Clin. Immunol. 115:S107-S107(2005)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 19-53; 62-72; 75-130; 134-154 AND 312-381, IDENTIFICATION BY MASS SPECTROMETRY, ALLERGEN.
      Tissue: Venom and Venom gland.
    2. "Molecular cloning of honeybee venom allergen acid phosphatase."
      Zhang Q., Liu Z., Wu Y.
      Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Venom gland.
    3. "Insights into social insects from the genome of the honeybee Apis mellifera."
      Honeybee genome sequencing consortium
      Nature 443:931-949(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Molecular cloning and expression in insect cells of honeybee venom allergen acid phosphatase (Api m 3)."
      Grunwald T., Bockisch B., Spillner E., Ring J., Bredehorst R., Ollert M.W.
      J. Allergy Clin. Immunol. 117:848-854(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-388, ALLERGEN.
      Tissue: Venom gland.
    5. Cited for: REVIEW.

    Entry informationi

    Entry nameiACPH1_APIME
    AccessioniPrimary (citable) accession number: Q5BLY5
    Secondary accession number(s): B6E2X9, Q4TUB9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 30, 2010
    Last sequence update: April 12, 2005
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Allergens
      Nomenclature of allergens and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3