Q5BLY5 (ACPH1_APIME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Venom acid phosphatase Acph-1 EC=3.1.3.2 Alternative name(s): Allergen=Api m 3
Organism	Apis mellifera (Honeybee) [Reference proteome]
Taxonomic identifier	7460 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Apoidea › Apidae › Apinae › Apini › Apis

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Allergenic properties	Causes an allergic reaction in human. Ref.1 Ref.4
Sequence similarities	Belongs to the histidine acid phosphatase family.

Ontologies

Keywords
Cellular component	Secreted
Disease	Allergen
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acid phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	Potential
	Chain	16 – 388	373	Venom acid phosphatase Acph-1		PRO_5000095341
Sites
	Active site	26	1	Nucleophile By similarity
	Active site	273	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	182	1	N-linked (GlcNAc...) Potential
	Glycosylation	228	1	N-linked (GlcNAc...) Potential
	Glycosylation	366	1	N-linked (GlcNAc...) Potential
	Disulfide bond	145 ↔ 355		By similarity
	Disulfide bond	330 ↔ 334		By similarity
Experimental info
	Sequence conflict	152	1	L → F in ACI25605. Ref.2
	Sequence conflict	278	1	S → A in ACI25605. Ref.2
	Sequence conflict	323	1	R → G in ACI25605. Ref.2
	Sequence conflict	360	1	V → A in ACI25605. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q5BLY5 [UniParc]. Last modified April 12, 2005. Version 1. Checksum: DD3327ECC2B03C6B	FASTA	388	45,389
	10 20 30 40 50 60 MSVIAILAMV VGVQAELKQI NVIFRHGDRI PDEKNEMYPK DPYLYYDFYP LERGELTNSG 70 80 90 100 110 120 KMREYQLGQF LRERYGDFLG DIYTEESVSA LSSFYDRTKM SLQLVLAALY PPNKLQQWNE 130 140 150 160 170 180 DLNWQPIATK YLRRYEDNIF LPEDCLLFTI ELDRVLESPR GKYEFSKYDK LKKKLEEWTG 190 200 210 220 230 240 KNITTPWDYY YIYHTLVAEQ SYGLTLPSWT NNIFPRGELF DATVFTYNIT NSTPLLKKLY 250 260 270 280 290 300 GGPLLRIFTK HMLDVVSGTQ KKKRKIYLFS GHESNIASVL HALQLYYPHV PEYSSSIIME 310 320 330 340 350 360 LHNIEGTHYV KIVYYLGIPS EARELQLPGC EVLCPLYKYL QLIENVIPSN EELICDKRFV 370 380 DESANNLSIE ELDFVKLNLI RIAGTENK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and characterization of honeybee venom acid phosphatase." Hoffman D.R., Weimer E.T., Sakell R.H., Schmidt M. J. Allergy Clin. Immunol. 115:S107-S107(2005) Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 19-53; 62-72; 75-130; 134-154 AND 312-381, MASS SPECTROMETRY, ALLERGEN. Tissue: Venom and Venom gland.
[2]	"Molecular cloning of honeybee venom allergen acid phosphatase." Zhang Q., Liu Z., Wu Y. Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Venom gland.
[3]	"Insights into social insects from the genome of the honeybee Apis mellifera." Honeybee genome sequencing consortium Nature 443:931-949(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Molecular cloning and expression in insect cells of honeybee venom allergen acid phosphatase (Api m 3)." Grunwald T., Bockisch B., Spillner E., Ring J., Bredehorst R., Ollert M.W. J. Allergy Clin. Immunol. 117:848-854(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-388, ALLERGEN. Tissue: Venom gland.
[5]	"Hymenoptera venom allergens." Hoffman D.R. Clin. Rev. Allergy Immunol. 30:109-128(2006) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY939855 mRNA. Translation: AAX33235.1. FJ200211 mRNA. Translation: ACI25605.1. DQ058012 mRNA. Translation: AAY57281.1.
RefSeq	NP_001013377.2. NM_001013359.2.
UniGene	Ame.7483.
3D structure databases
ProteinModelPortal	Q5BLY5.
ModBase	Search...
Protein family/group databases
Allergome	2778. Api m A1-A2-A3. 3090. Api m 3.0101. 47. Api m 3.
Proteomic databases
PaxDb	Q5BLY5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	GB12546-RA; GB12546-PA; GB12546.
GeneID	411830.
KEGG	ame:411830.
Organism-specific databases
CTD	48445.
Phylogenomic databases
eggNOG	NOG291245.
InParanoid	Q5BLY5.
OMA	GHENNIA.
OrthoDB	EOG4F4QT6.
Family and domain databases
InterPro	IPR000560. His_Pase_superF_clade-2. [Graphical view]
Pfam	PF00328. His_Phos_2. 1 hit. [Graphical view]
PROSITE	PS00616. HIS_ACID_PHOSPHAT_1. 1 hit. PS00778. HIS_ACID_PHOSPHAT_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACPH1_APIME

Accession

Primary (citable) accession number: Q5BLY5
Secondary accession number(s): B6E2X9, Q4TUB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 30, 2010
Last sequence update:	April 12, 2005
Last modified:	March 6, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)