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Q5BLY5 (ACPH1_APIME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Venom acid phosphatase Acph-1

EC=3.1.3.2
Alternative name(s):
Allergen=Api m 3
OrganismApis mellifera (Honeybee) [Reference proteome]
Taxonomic identifier7460 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataApoideaApidaeApis

Protein attributes

Sequence length388 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Subcellular location

Secreted.

Tissue specificity

Expressed by the venom gland.

Allergenic properties

Causes an allergic reaction in human. Ref.1 Ref.4

Sequence similarities

Belongs to the histidine acid phosphatase family.

Ontologies

Keywords
   Cellular componentSecreted
   DiseaseAllergen
   DomainSignal
   Molecular functionHydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionacid phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1515 Potential
Chain16 – 388373Venom acid phosphatase Acph-1
PRO_5000095341

Sites

Active site261Nucleophile By similarity
Active site2731Proton donor By similarity

Amino acid modifications

Glycosylation1821N-linked (GlcNAc...) Potential
Glycosylation2281N-linked (GlcNAc...) Potential
Glycosylation3661N-linked (GlcNAc...) Potential
Disulfide bond145 ↔ 355 By similarity
Disulfide bond330 ↔ 334 By similarity

Experimental info

Sequence conflict1521L → F in ACI25605. Ref.2
Sequence conflict2781S → A in ACI25605. Ref.2
Sequence conflict3231R → G in ACI25605. Ref.2
Sequence conflict3601V → A in ACI25605. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q5BLY5 [UniParc].

Last modified April 12, 2005. Version 1.
Checksum: DD3327ECC2B03C6B

FASTA38845,389
        10         20         30         40         50         60 
MSVIAILAMV VGVQAELKQI NVIFRHGDRI PDEKNEMYPK DPYLYYDFYP LERGELTNSG 

        70         80         90        100        110        120 
KMREYQLGQF LRERYGDFLG DIYTEESVSA LSSFYDRTKM SLQLVLAALY PPNKLQQWNE 

       130        140        150        160        170        180 
DLNWQPIATK YLRRYEDNIF LPEDCLLFTI ELDRVLESPR GKYEFSKYDK LKKKLEEWTG 

       190        200        210        220        230        240 
KNITTPWDYY YIYHTLVAEQ SYGLTLPSWT NNIFPRGELF DATVFTYNIT NSTPLLKKLY 

       250        260        270        280        290        300 
GGPLLRIFTK HMLDVVSGTQ KKKRKIYLFS GHESNIASVL HALQLYYPHV PEYSSSIIME 

       310        320        330        340        350        360 
LHNIEGTHYV KIVYYLGIPS EARELQLPGC EVLCPLYKYL QLIENVIPSN EELICDKRFV 

       370        380 
DESANNLSIE ELDFVKLNLI RIAGTENK 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and characterization of honeybee venom acid phosphatase."
Hoffman D.R., Weimer E.T., Sakell R.H., Schmidt M.
J. Allergy Clin. Immunol. 115:S107-S107(2005)
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 19-53; 62-72; 75-130; 134-154 AND 312-381, IDENTIFICATION BY MASS SPECTROMETRY, ALLERGEN.
Tissue: Venom and Venom gland.
[2]"Molecular cloning of honeybee venom allergen acid phosphatase."
Zhang Q., Liu Z., Wu Y.
Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Venom gland.
[3]"Insights into social insects from the genome of the honeybee Apis mellifera."
Honeybee genome sequencing consortium
Nature 443:931-949(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Molecular cloning and expression in insect cells of honeybee venom allergen acid phosphatase (Api m 3)."
Grunwald T., Bockisch B., Spillner E., Ring J., Bredehorst R., Ollert M.W.
J. Allergy Clin. Immunol. 117:848-854(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-388, ALLERGEN.
Tissue: Venom gland.
[5]"Hymenoptera venom allergens."
Hoffman D.R.
Clin. Rev. Allergy Immunol. 30:109-128(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY939855 mRNA. Translation: AAX33235.1.
FJ200211 mRNA. Translation: ACI25605.1.
DQ058012 mRNA. Translation: AAY57281.1.
RefSeqNP_001013377.2. NM_001013359.2.
UniGeneAme.7483.

3D structure databases

ProteinModelPortalQ5BLY5.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

Allergome2778. Api m A1-A2-A3.
3090. Api m 3.0101.
47. Api m 3.

Proteomic databases

PaxDbQ5BLY5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaGB12546-RA; GB12546-PA; GB12546.
GeneID411830.
KEGGame:411830.

Organism-specific databases

CTD48445.

Phylogenomic databases

eggNOGNOG291245.
InParanoidQ5BLY5.
OMAGHENNIA.

Family and domain databases

InterProIPR000560. His_Pase_superF_clade-2.
[Graphical view]
PfamPF00328. His_Phos_2. 1 hit.
[Graphical view]
PROSITEPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameACPH1_APIME
AccessionPrimary (citable) accession number: Q5BLY5
Secondary accession number(s): B6E2X9, Q4TUB9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: April 12, 2005
Last modified: April 16, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Allergens

Nomenclature of allergens and list of entries