Reviewed, UniProtKB/Swiss-Prot Q5BLE8 (RETST_DANRE)

Names and origin

Protein names

Recommended name:
    Putative all-trans-retinol 13,14-reductase
    EC=1.3.99.23
Alternative name(s):
    All-trans-13,14-dihydroretinol saturase
      Short name=RetSat

Gene names

Name:	retsat
ORF Names:	zgc:113107

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	607 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Retinol saturase carrying out the saturation of the 13-14 double bond of all-trans-retinol to produce all-trans-13,14-dihydroretinol By similarity.
Catalytic activity	All-trans-13,14-dihydroretinol + acceptor = all-trans-retinol + reduced acceptor.
Cofactor	NAD, NADP, or FAD By similarity.
Subcellular location	Membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the carotenoid/retinoid oxidoreductase family. CrtISO subfamily.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	all-trans-retinol 13,14-reductase activity Inferred from direct assay. Source: ZFIN
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 607	585	Putative all-trans-retinol 13,14-reductase		PRO_0000225669
Regions
	Nucleotide binding	65 – 93	29	FAD or NAD or NADP Potential
Experimental info
	Sequence conflict	572	1	L → S in AAH78372. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q5BLE8-1 [UniParc]. Last modified April 12, 2005. Version 1. Checksum: 4B387672D3AA2E02	FASTA	607	67,937
	10 20 30 40 50 60 MWFAVVAIFL ALVAFLYRYV VGSGPNPFAI DTREPLKPMV FDRKLKNKVL KQGFLASRVP 70 80 90 100 110 120 EDLDAVVVGS GIGGLAIAVL LAKVGKKVLV LEQHDRAGGC CHTFKEQGFE FDVGIHYIGE 130 140 150 160 170 180 LSNHKPLRCI IDQMTNGQLQ WDPLENPFDN VVIGPPENRR IYQIYSGRKR YMDELKKCFP 190 200 210 220 230 240 GEEKAIDEYV RLCKEVGQGV WVMVLLKFLP TPIANFLVRT GLANRLTSFS RYASRSLTDV 250 260 270 280 290 300 VNELTQNKDL RAVLSYIFGT YGKIPKEASF SMHSLIVNHY MNGAWYPKGG ATEIAYHMIP 310 320 330 340 350 360 IIEKAGGAVL VRAPVNRILL NDAKEAIGVS VLKGQEEVHV RAPIVISDAG IFNTYEYLLP 370 380 390 400 410 420 KDVQTMPAIQ KQLSMLQHGD SGLSIFIGLD GTKEELGLKA DNYFIYPENN IDELLEDYRS 430 440 450 460 470 480 GNREESAKKN PLIFVASPSA KDSTWPERTP GKSTLTVVSF ANYEWFEEWK DDKVKNRSTD 490 500 510 520 530 540 YKQLKELFIN YILEAVTEIY PKIKDRIEYV DAGTPITNQH YIAAPRGEIY GADHGIPRFS 550 560 570 580 590 600 AELNATIRAQ TPIKNLYLTG QDLMLCGFAG ALTGALTCGS VILNRNLHLE AFSLAKRVQN GNNKKKT

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC078372 mRNA. Translation: AAH78372.1. BC090469 mRNA. Translation: AAH90469.1.
IPI	IPI00495382.
RefSeq	NP_001015061.1.
UniGene	Dr.80063
3D structure databases
SMR	Q5BLE8. Positions 18-120, 59-365.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5BLE8.
Genome annotation databases
Ensembl	ENSDART00000013167; ENSDARP00000024120; ENSDARG00000018600; Danio rerio. [Genome view]
GeneID	325922.
KEGG	dre:325922.
Organism-specific databases
CTD	325922.
ZFIN	ZDB-GENE-050320-11. retsat.
Phylogenomic databases
eggNOG	fiNOG06874.
HOGENOM	HBG443934.
HOVERGEN	Q5BLE8.
InParanoid	Q5BLE8.
OMA	GQDIFTC.
OrthoDB	EOG912PQT.
Enzyme and pathway databases
BRENDA	1.3.99.23. 96826.
Gene expression databases
ArrayExpress	Q5BLE8.
Bgee	Q5BLE8.
Family and domain databases
InterPro	IPR002937. Amino_oxidase. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RETST_DANRE

Accession

Primary (citable) accession number: Q5BLE8
Secondary accession number(s): Q6DBT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	April 12, 2005
Last modified:	January 19, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project