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Protein

Peroxisome biogenesis factor 1

Gene

Pex1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for stability of PEX5 and protein import into the peroxisome matrix. Anchored by PEX26 to peroxisome membranes, possibly to form heteromeric AAA ATPase complexes required for the import of proteins into peroxisomes (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi600 – 607ATPSequence analysis8
Nucleotide bindingi882 – 889ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Peroxisome biogenesis, Protein transport, Transport

Keywords - Ligandi

ATP-binding, Lipid-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisome biogenesis factor 1
Alternative name(s):
Peroxin-1
Gene namesi
Name:Pex1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1918632. Pex1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi135R → A: Loss of phospholipid-binding. 1 Publication1
Mutagenesisi174K → A: No effect on phospholipid-binding. 1 Publication1

Keywords - Diseasei

Peroxisome biogenesis disorder

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003049311 – 1284Peroxisome biogenesis factor 1Add BLAST1284

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1182PhosphoserineBy similarity1
Modified residuei1210PhosphoserineBy similarity1
Modified residuei1212PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ5BL07.
PaxDbiQ5BL07.
PeptideAtlasiQ5BL07.
PRIDEiQ5BL07.

PTM databases

iPTMnetiQ5BL07.
PhosphoSitePlusiQ5BL07.

Expressioni

Gene expression databases

BgeeiENSMUSG00000005907.
CleanExiMM_PEX1.
ExpressionAtlasiQ5BL07. baseline and differential.
GenevisibleiQ5BL07. MM.

Interactioni

Subunit structurei

Interacts directly with PEX6. Interacts indirectly with PEX26, via its interaction with PEX6 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi214674. 1 interactor.
IntActiQ5BL07. 3 interactors.
STRINGi10090.ENSMUSP00000006061.

Structurei

Secondary structure

11284
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi15 – 22Combined sources8
Beta strandi25 – 27Combined sources3
Beta strandi29 – 31Combined sources3
Helixi33 – 38Combined sources6
Beta strandi47 – 53Combined sources7
Beta strandi56 – 58Combined sources3
Beta strandi60 – 62Combined sources3
Beta strandi73 – 77Combined sources5
Helixi78 – 83Combined sources6
Beta strandi91 – 96Combined sources6
Beta strandi104 – 112Combined sources9
Helixi113 – 121Combined sources9
Helixi126 – 133Combined sources8
Beta strandi142 – 150Combined sources9
Beta strandi152 – 162Combined sources11
Beta strandi165 – 168Combined sources4
Beta strandi174 – 177Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WLFX-ray2.05A3-181[»]
ProteinModelPortaliQ5BL07.
SMRiQ5BL07.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5BL07.

Family & Domainsi

Domaini

The N-terminal domain shows evolutionary conservation with that of VCP, and is able to bind phospholipids with a preference for phosphatidylinositol monophosphates.

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0735. Eukaryota.
COG0464. LUCA.
GeneTreeiENSGT00550000075032.
HOGENOMiHOG000252959.
HOVERGENiHBG008169.
InParanoidiQ5BL07.
KOiK13338.
OMAiNIEMHAV.
OrthoDBiEOG091G08H7.
PhylomeDBiQ5BL07.
TreeFamiTF106447.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR029067. CDC48_domain_2-like.
IPR027417. P-loop_NTPase.
IPR015343. PEX-N_a/b.
IPR015342. PEX-N_psi_beta-barrel.
IPR025653. Pex1.
[Graphical view]
PANTHERiPTHR23077:SF12. PTHR23077:SF12. 4 hits.
PfamiPF00004. AAA. 2 hits.
PF09262. PEX-1N. 1 hit.
PF09263. PEX-2N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5BL07-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWSSDRLAGA GSGGAVVTVA FTNARDCFLH LPRRLVAQLH LLQNQAIEVA
60 70 80 90 100
SDHQPTYLSW VEGRHFNDQS ENVAEINRQV GQKLGLSSGD QVFLRPCSHV
110 120 130 140 150
VSCQQVEVEP LSADDWEILE LHAISLEQHL LDQIRIVFPK AVVPIWVDQQ
160 170 180 190 200
TYIFIQIVTL MPAAPYGRLE TNTKLLIQPK TRQAKESTFP KEGDAHGQVH
210 220 230 240 250
SYGREQKGLS KELQTRQLHT NSEGITASNG RDPKVPGGPL KPSWWAVLGS
260 270 280 290 300
MLSFGPDSKQ ESAWGSLELG AFKNMQSQAA PLEGTFRVCQ VQPPSARTTT
310 320 330 340 350
ATSVFHKHCT AHVFPWDQEY FDVEPSFTVT YGKLVKLHSP KQQQDKSKQG
360 370 380 390 400
VLLPDKEKQL SKSPDHKQIS SNRSEEAAEA CVLKVVWNGL EELKNATEFT
410 420 430 440 450
ESLELLHRGK VWIPDDLRKR LNIEMHAVVR ITPLETTPKI PRSLKLQPRE
460 470 480 490 500
NLPKDVNEET IKTVFSSWVQ QSATTMLPLV ISKEERIKLE IKDGLREFSL
510 520 530 540 550
STVHSQEKEK EEGKTVFVLS SILLQKISVQ VLLEPMIKEE QSAEIDFLLP
560 570 580 590 600
SLTLSSLGGV SALGASAMEH ITHSLLGRPL SRQLMALVAG LRNGALLITG
610 620 630 640 650
GKGSGKSTFA KAICKEAQDT LDARVETVDC KALRGKRLES IQKALEVAFS
660 670 680 690 700
EAAWRQPSVI LLDDLDLIAG LPSVPEQEHS PEAVQSQRLA HALNDMIKEF
710 720 730 740 750
VSTGSLVALI ATSQLQQSLH PSLVSAQGIH TFQCVQHLQP PNPEQRCEIL
760 770 780 790 800
HSVVKNKLGC DISNFPDLDL QCIAKDTEAF VARDFTVLVD RAIHSSLSRQ
810 820 830 840 850
HSSSREDLTL TTSDFQKALR GFLPASLRNV NLHKPRDLGW DKIGGLHEVR
860 870 880 890 900
QILMDTIQLP AKYPELFANL PIRQRTGILL YGPPGTGKTL LAGVVARESG
910 920 930 940 950
MNFISIKGPE LLSKYIGASE QAVRDVFIRA QAAKPCILFF DEFESIAPRR
960 970 980 990 1000
GHDNTGVTDR VVNQLLTQLD GVEGLQGVYV LAATSRPDLI DPALLRPGRL
1010 1020 1030 1040 1050
DKCVYCPPPD QVSRLEILTV LSKSLALADD VDLQHVASVT DSFTGADLKA
1060 1070 1080 1090 1100
LLYNAQLEAL QGRLLPSGLP DGGSSSDSDL SLSSMVFLNH SSGSDDSAGD
1110 1120 1130 1140 1150
GECGLEQSLL SLEMSEILPD ESKFNMYRLY FGSSYESELG NGTPSDLSSH
1160 1170 1180 1190 1200
CLSAPSSVTQ DLPAAPGKDP LFTQHPVFRT PSQEGCQDLT QEQRDQLRAE
1210 1220 1230 1240 1250
ISIIKGRYRS QSGEDESLNQ PGPIKTTFAI SQAHLMTALA HTRPSISEDE
1260 1270 1280
GKEFAELYEN FQNPKKRKNQ SGTVFRTGQK VTLA
Length:1,284
Mass (Da):141,428
Last modified:October 2, 2007 - v2
Checksum:i29B6C9B6E93FA6EF
GO
Isoform 2 (identifier: Q5BL07-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     413-452: Missing.

Note: No experimental confirmation available.
Show »
Length:1,244
Mass (Da):136,743
Checksum:i9B1073BBA4CC4106
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_028131413 – 452Missing in isoform 2. 1 PublicationAdd BLAST40

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC090845 mRNA. Translation: AAH90845.1.
AK017309 mRNA. Translation: BAB30684.1.
AK149599 mRNA. Translation: BAE28984.1.
CCDSiCCDS19065.1. [Q5BL07-2]
CCDS80201.1. [Q5BL07-1]
RefSeqiNP_001280735.1. NM_001293806.1. [Q5BL07-1]
NP_082053.1. NM_027777.2. [Q5BL07-2]
UniGeneiMm.379196.

Genome annotation databases

EnsembliENSMUST00000006061; ENSMUSP00000006061; ENSMUSG00000005907. [Q5BL07-2]
ENSMUST00000121291; ENSMUSP00000113304; ENSMUSG00000005907. [Q5BL07-1]
GeneIDi71382.
KEGGimmu:71382.
UCSCiuc008whe.2. mouse. [Q5BL07-1]
uc008whf.2. mouse. [Q5BL07-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC090845 mRNA. Translation: AAH90845.1.
AK017309 mRNA. Translation: BAB30684.1.
AK149599 mRNA. Translation: BAE28984.1.
CCDSiCCDS19065.1. [Q5BL07-2]
CCDS80201.1. [Q5BL07-1]
RefSeqiNP_001280735.1. NM_001293806.1. [Q5BL07-1]
NP_082053.1. NM_027777.2. [Q5BL07-2]
UniGeneiMm.379196.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WLFX-ray2.05A3-181[»]
ProteinModelPortaliQ5BL07.
SMRiQ5BL07.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214674. 1 interactor.
IntActiQ5BL07. 3 interactors.
STRINGi10090.ENSMUSP00000006061.

PTM databases

iPTMnetiQ5BL07.
PhosphoSitePlusiQ5BL07.

Proteomic databases

EPDiQ5BL07.
PaxDbiQ5BL07.
PeptideAtlasiQ5BL07.
PRIDEiQ5BL07.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000006061; ENSMUSP00000006061; ENSMUSG00000005907. [Q5BL07-2]
ENSMUST00000121291; ENSMUSP00000113304; ENSMUSG00000005907. [Q5BL07-1]
GeneIDi71382.
KEGGimmu:71382.
UCSCiuc008whe.2. mouse. [Q5BL07-1]
uc008whf.2. mouse. [Q5BL07-2]

Organism-specific databases

CTDi5189.
MGIiMGI:1918632. Pex1.

Phylogenomic databases

eggNOGiKOG0735. Eukaryota.
COG0464. LUCA.
GeneTreeiENSGT00550000075032.
HOGENOMiHOG000252959.
HOVERGENiHBG008169.
InParanoidiQ5BL07.
KOiK13338.
OMAiNIEMHAV.
OrthoDBiEOG091G08H7.
PhylomeDBiQ5BL07.
TreeFamiTF106447.

Miscellaneous databases

EvolutionaryTraceiQ5BL07.
PROiQ5BL07.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000005907.
CleanExiMM_PEX1.
ExpressionAtlasiQ5BL07. baseline and differential.
GenevisibleiQ5BL07. MM.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR029067. CDC48_domain_2-like.
IPR027417. P-loop_NTPase.
IPR015343. PEX-N_a/b.
IPR015342. PEX-N_psi_beta-barrel.
IPR025653. Pex1.
[Graphical view]
PANTHERiPTHR23077:SF12. PTHR23077:SF12. 4 hits.
PfamiPF00004. AAA. 2 hits.
PF09262. PEX-1N. 1 hit.
PF09263. PEX-2N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPEX1_MOUSE
AccessioniPrimary (citable) accession number: Q5BL07
Secondary accession number(s): Q3UEC7, Q9CU85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: November 30, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.