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Q5BL07

- PEX1_MOUSE

UniProt

Q5BL07 - PEX1_MOUSE

Protein

Peroxisome biogenesis factor 1

Gene

Pex1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 2 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    Required for stability of PEX5 and protein import into the peroxisome matrix. Anchored by PEX26 to peroxisome membranes, possibly to form heteromeric AAA ATPase complexes required for the import of proteins into peroxisomes By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi600 – 6078ATPSequence Analysis
    Nucleotide bindingi882 – 8898ATPSequence Analysis

    GO - Molecular functioni

    1. ATPase activity, coupled Source: Ensembl
    2. ATP binding Source: UniProtKB-KW
    3. lipid binding Source: UniProtKB-KW

    GO - Biological processi

    1. microtubule-based peroxisome localization Source: Ensembl
    2. protein import into peroxisome matrix Source: Ensembl

    Keywords - Biological processi

    Peroxisome biogenesis, Protein transport, Transport

    Keywords - Ligandi

    ATP-binding, Lipid-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peroxisome biogenesis factor 1
    Alternative name(s):
    Peroxin-1
    Gene namesi
    Name:Pex1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1918632. Pex1.

    Subcellular locationi

    Cytoplasm By similarity. Peroxisome membrane By similarity
    Note: Associated with peroxisomal membranes.By similarity

    GO - Cellular componenti

    1. cytosol Source: Ensembl
    2. nucleus Source: Ensembl
    3. peroxisomal membrane Source: UniProtKB-SubCell
    4. peroxisome Source: HGNC

    Keywords - Cellular componenti

    Cytoplasm, Membrane, Peroxisome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi135 – 1351R → A: Loss of phospholipid-binding. 1 Publication
    Mutagenesisi174 – 1741K → A: No effect on phospholipid-binding. 1 Publication

    Keywords - Diseasei

    Peroxisome biogenesis disorder

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12841284Peroxisome biogenesis factor 1PRO_0000304931Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki834 – 834Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Modified residuei1210 – 12101PhosphoserineBy similarity

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ5BL07.
    PaxDbiQ5BL07.
    PRIDEiQ5BL07.

    PTM databases

    PhosphoSiteiQ5BL07.

    Expressioni

    Gene expression databases

    ArrayExpressiQ5BL07.
    BgeeiQ5BL07.
    CleanExiMM_PEX1.
    GenevestigatoriQ5BL07.

    Interactioni

    Subunit structurei

    Interacts directly with PEX6. Interacts indirectly with PEX26, via its interaction with PEX6 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi214674. 1 interaction.

    Structurei

    Secondary structure

    1
    1284
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi15 – 228
    Beta strandi25 – 273
    Beta strandi29 – 313
    Helixi33 – 386
    Beta strandi47 – 537
    Beta strandi56 – 583
    Beta strandi60 – 623
    Beta strandi73 – 775
    Helixi78 – 836
    Beta strandi91 – 966
    Beta strandi104 – 1129
    Helixi113 – 1219
    Helixi126 – 1338
    Beta strandi142 – 1509
    Beta strandi152 – 16211
    Beta strandi165 – 1684
    Beta strandi174 – 1774

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1WLFX-ray2.05A3-181[»]
    ProteinModelPortaliQ5BL07.
    SMRiQ5BL07. Positions 13-179.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ5BL07.

    Family & Domainsi

    Domaini

    The N-terminal domain shows evolutionary conservation with that of VCP, and is able to bind phospholipids with a preference for phosphatidylinositol monophosphates.

    Sequence similaritiesi

    Belongs to the AAA ATPase family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0464.
    GeneTreeiENSGT00550000075032.
    HOGENOMiHOG000252959.
    HOVERGENiHBG008169.
    InParanoidiQ5BL07.
    KOiK13338.
    OMAiVHSWEKE.
    OrthoDBiEOG7W9RT0.
    PhylomeDBiQ5BL07.
    TreeFamiTF106447.

    Family and domain databases

    Gene3Di3.10.330.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR029067. CDC48_domain_2-like.
    IPR027417. P-loop_NTPase.
    IPR015343. Peroxisome_synth_fac_1_a/b.
    IPR015342. PEX-1N.
    IPR025653. Pex1.
    [Graphical view]
    PANTHERiPTHR23077:SF12. PTHR23077:SF12. 1 hit.
    PfamiPF00004. AAA. 2 hits.
    PF09262. PEX-1N. 1 hit.
    PF09263. PEX-2N. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 2 hits.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF54585. SSF54585. 1 hit.
    PROSITEiPS00674. AAA. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q5BL07-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MWSSDRLAGA GSGGAVVTVA FTNARDCFLH LPRRLVAQLH LLQNQAIEVA     50
    SDHQPTYLSW VEGRHFNDQS ENVAEINRQV GQKLGLSSGD QVFLRPCSHV 100
    VSCQQVEVEP LSADDWEILE LHAISLEQHL LDQIRIVFPK AVVPIWVDQQ 150
    TYIFIQIVTL MPAAPYGRLE TNTKLLIQPK TRQAKESTFP KEGDAHGQVH 200
    SYGREQKGLS KELQTRQLHT NSEGITASNG RDPKVPGGPL KPSWWAVLGS 250
    MLSFGPDSKQ ESAWGSLELG AFKNMQSQAA PLEGTFRVCQ VQPPSARTTT 300
    ATSVFHKHCT AHVFPWDQEY FDVEPSFTVT YGKLVKLHSP KQQQDKSKQG 350
    VLLPDKEKQL SKSPDHKQIS SNRSEEAAEA CVLKVVWNGL EELKNATEFT 400
    ESLELLHRGK VWIPDDLRKR LNIEMHAVVR ITPLETTPKI PRSLKLQPRE 450
    NLPKDVNEET IKTVFSSWVQ QSATTMLPLV ISKEERIKLE IKDGLREFSL 500
    STVHSQEKEK EEGKTVFVLS SILLQKISVQ VLLEPMIKEE QSAEIDFLLP 550
    SLTLSSLGGV SALGASAMEH ITHSLLGRPL SRQLMALVAG LRNGALLITG 600
    GKGSGKSTFA KAICKEAQDT LDARVETVDC KALRGKRLES IQKALEVAFS 650
    EAAWRQPSVI LLDDLDLIAG LPSVPEQEHS PEAVQSQRLA HALNDMIKEF 700
    VSTGSLVALI ATSQLQQSLH PSLVSAQGIH TFQCVQHLQP PNPEQRCEIL 750
    HSVVKNKLGC DISNFPDLDL QCIAKDTEAF VARDFTVLVD RAIHSSLSRQ 800
    HSSSREDLTL TTSDFQKALR GFLPASLRNV NLHKPRDLGW DKIGGLHEVR 850
    QILMDTIQLP AKYPELFANL PIRQRTGILL YGPPGTGKTL LAGVVARESG 900
    MNFISIKGPE LLSKYIGASE QAVRDVFIRA QAAKPCILFF DEFESIAPRR 950
    GHDNTGVTDR VVNQLLTQLD GVEGLQGVYV LAATSRPDLI DPALLRPGRL 1000
    DKCVYCPPPD QVSRLEILTV LSKSLALADD VDLQHVASVT DSFTGADLKA 1050
    LLYNAQLEAL QGRLLPSGLP DGGSSSDSDL SLSSMVFLNH SSGSDDSAGD 1100
    GECGLEQSLL SLEMSEILPD ESKFNMYRLY FGSSYESELG NGTPSDLSSH 1150
    CLSAPSSVTQ DLPAAPGKDP LFTQHPVFRT PSQEGCQDLT QEQRDQLRAE 1200
    ISIIKGRYRS QSGEDESLNQ PGPIKTTFAI SQAHLMTALA HTRPSISEDE 1250
    GKEFAELYEN FQNPKKRKNQ SGTVFRTGQK VTLA 1284
    Length:1,284
    Mass (Da):141,428
    Last modified:October 2, 2007 - v2
    Checksum:i29B6C9B6E93FA6EF
    GO
    Isoform 2 (identifier: Q5BL07-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         413-452: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,244
    Mass (Da):136,743
    Checksum:i9B1073BBA4CC4106
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei413 – 45240Missing in isoform 2. 1 PublicationVSP_028131Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC090845 mRNA. Translation: AAH90845.1.
    AK017309 mRNA. Translation: BAB30684.1.
    AK149599 mRNA. Translation: BAE28984.1.
    CCDSiCCDS19065.1. [Q5BL07-2]
    RefSeqiNP_082053.1. NM_027777.1. [Q5BL07-2]
    XP_006503668.1. XM_006503605.1. [Q5BL07-1]
    UniGeneiMm.379196.

    Genome annotation databases

    EnsembliENSMUST00000006061; ENSMUSP00000006061; ENSMUSG00000005907. [Q5BL07-2]
    ENSMUST00000121291; ENSMUSP00000113304; ENSMUSG00000005907. [Q5BL07-1]
    GeneIDi71382.
    KEGGimmu:71382.
    UCSCiuc008whf.1. mouse. [Q5BL07-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC090845 mRNA. Translation: AAH90845.1 .
    AK017309 mRNA. Translation: BAB30684.1 .
    AK149599 mRNA. Translation: BAE28984.1 .
    CCDSi CCDS19065.1. [Q5BL07-2 ]
    RefSeqi NP_082053.1. NM_027777.1. [Q5BL07-2 ]
    XP_006503668.1. XM_006503605.1. [Q5BL07-1 ]
    UniGenei Mm.379196.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1WLF X-ray 2.05 A 3-181 [» ]
    ProteinModelPortali Q5BL07.
    SMRi Q5BL07. Positions 13-179.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 214674. 1 interaction.

    PTM databases

    PhosphoSitei Q5BL07.

    Proteomic databases

    MaxQBi Q5BL07.
    PaxDbi Q5BL07.
    PRIDEi Q5BL07.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000006061 ; ENSMUSP00000006061 ; ENSMUSG00000005907 . [Q5BL07-2 ]
    ENSMUST00000121291 ; ENSMUSP00000113304 ; ENSMUSG00000005907 . [Q5BL07-1 ]
    GeneIDi 71382.
    KEGGi mmu:71382.
    UCSCi uc008whf.1. mouse. [Q5BL07-2 ]

    Organism-specific databases

    CTDi 5189.
    MGIi MGI:1918632. Pex1.

    Phylogenomic databases

    eggNOGi COG0464.
    GeneTreei ENSGT00550000075032.
    HOGENOMi HOG000252959.
    HOVERGENi HBG008169.
    InParanoidi Q5BL07.
    KOi K13338.
    OMAi VHSWEKE.
    OrthoDBi EOG7W9RT0.
    PhylomeDBi Q5BL07.
    TreeFami TF106447.

    Miscellaneous databases

    EvolutionaryTracei Q5BL07.
    NextBioi 333669.
    PROi Q5BL07.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q5BL07.
    Bgeei Q5BL07.
    CleanExi MM_PEX1.
    Genevestigatori Q5BL07.

    Family and domain databases

    Gene3Di 3.10.330.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR029067. CDC48_domain_2-like.
    IPR027417. P-loop_NTPase.
    IPR015343. Peroxisome_synth_fac_1_a/b.
    IPR015342. PEX-1N.
    IPR025653. Pex1.
    [Graphical view ]
    PANTHERi PTHR23077:SF12. PTHR23077:SF12. 1 hit.
    Pfami PF00004. AAA. 2 hits.
    PF09262. PEX-1N. 1 hit.
    PF09263. PEX-2N. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 2 hits.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF54585. SSF54585. 1 hit.
    PROSITEi PS00674. AAA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6.
      Tissue: Brain.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-505 AND 795-1284 (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Head and Liver.
    3. "The common phospholipid-binding activity of the N-terminal domains of PEX1 and VCP/p97."
      Shiozawa K., Goda N., Shimizu T., Mizuguchi K., Kondo N., Shimozawa N., Shirakawa M., Hiroaki H.
      FEBS J. 273:4959-4971(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHOLIPID BINDING, MUTAGENESIS OF ARG-135 AND LYS-174.
    4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    5. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
      Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
      J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "Structure of the N-terminal domain of PEX1 AAA-ATPase. Characterization of a putative adaptor-binding domain."
      Shiozawa K., Maita N., Tomii K., Seto A., Goda N., Akiyama Y., Shimizu T., Shirakawa M., Hiroaki H.
      J. Biol. Chem. 279:50060-50068(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 3-181.

    Entry informationi

    Entry nameiPEX1_MOUSE
    AccessioniPrimary (citable) accession number: Q5BL07
    Secondary accession number(s): Q3UEC7, Q9CU85
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 90 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3