Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q5BL07 (PEX1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peroxisome biogenesis factor 1
Alternative name(s):
Peroxin-1
Gene names
Name:Pex1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1284 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for stability of PEX5 and protein import into the peroxisome matrix. Anchored by PEX26 to peroxisome membranes, possibly to form heteromeric AAA ATPase complexes required for the import of proteins into peroxisomes By similarity.

Subunit structure

Interacts directly with PEX6. Interacts indirectly with PEX26, via its interaction with PEX6 By similarity.

Subcellular location

Cytoplasm By similarity. Peroxisome membrane By similarity. Note: Associated with peroxisomal membranes By similarity.

Domain

The N-terminal domain shows evolutionary conservation with that of VCP, and is able to bind phospholipids with a preference for phosphatidylinositol monophosphates.

Sequence similarities

Belongs to the AAA ATPase family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q5BL07-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q5BL07-2)

The sequence of this isoform differs from the canonical sequence as follows:
     413-452: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12841284Peroxisome biogenesis factor 1
PRO_0000304931

Regions

Nucleotide binding600 – 6078ATP Potential
Nucleotide binding882 – 8898ATP Potential

Amino acid modifications

Modified residue12101Phosphoserine By similarity
Cross-link834Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Natural variations

Alternative sequence413 – 45240Missing in isoform 2.
VSP_028131

Experimental info

Mutagenesis1351R → A: Loss of phospholipid-binding. Ref.3
Mutagenesis1741K → A: No effect on phospholipid-binding. Ref.3

Secondary structure

................................. 1284
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 2, 2007. Version 2.
Checksum: 29B6C9B6E93FA6EF

FASTA1,284141,428
        10         20         30         40         50         60 
MWSSDRLAGA GSGGAVVTVA FTNARDCFLH LPRRLVAQLH LLQNQAIEVA SDHQPTYLSW 

        70         80         90        100        110        120 
VEGRHFNDQS ENVAEINRQV GQKLGLSSGD QVFLRPCSHV VSCQQVEVEP LSADDWEILE 

       130        140        150        160        170        180 
LHAISLEQHL LDQIRIVFPK AVVPIWVDQQ TYIFIQIVTL MPAAPYGRLE TNTKLLIQPK 

       190        200        210        220        230        240 
TRQAKESTFP KEGDAHGQVH SYGREQKGLS KELQTRQLHT NSEGITASNG RDPKVPGGPL 

       250        260        270        280        290        300 
KPSWWAVLGS MLSFGPDSKQ ESAWGSLELG AFKNMQSQAA PLEGTFRVCQ VQPPSARTTT 

       310        320        330        340        350        360 
ATSVFHKHCT AHVFPWDQEY FDVEPSFTVT YGKLVKLHSP KQQQDKSKQG VLLPDKEKQL 

       370        380        390        400        410        420 
SKSPDHKQIS SNRSEEAAEA CVLKVVWNGL EELKNATEFT ESLELLHRGK VWIPDDLRKR 

       430        440        450        460        470        480 
LNIEMHAVVR ITPLETTPKI PRSLKLQPRE NLPKDVNEET IKTVFSSWVQ QSATTMLPLV 

       490        500        510        520        530        540 
ISKEERIKLE IKDGLREFSL STVHSQEKEK EEGKTVFVLS SILLQKISVQ VLLEPMIKEE 

       550        560        570        580        590        600 
QSAEIDFLLP SLTLSSLGGV SALGASAMEH ITHSLLGRPL SRQLMALVAG LRNGALLITG 

       610        620        630        640        650        660 
GKGSGKSTFA KAICKEAQDT LDARVETVDC KALRGKRLES IQKALEVAFS EAAWRQPSVI 

       670        680        690        700        710        720 
LLDDLDLIAG LPSVPEQEHS PEAVQSQRLA HALNDMIKEF VSTGSLVALI ATSQLQQSLH 

       730        740        750        760        770        780 
PSLVSAQGIH TFQCVQHLQP PNPEQRCEIL HSVVKNKLGC DISNFPDLDL QCIAKDTEAF 

       790        800        810        820        830        840 
VARDFTVLVD RAIHSSLSRQ HSSSREDLTL TTSDFQKALR GFLPASLRNV NLHKPRDLGW 

       850        860        870        880        890        900 
DKIGGLHEVR QILMDTIQLP AKYPELFANL PIRQRTGILL YGPPGTGKTL LAGVVARESG 

       910        920        930        940        950        960 
MNFISIKGPE LLSKYIGASE QAVRDVFIRA QAAKPCILFF DEFESIAPRR GHDNTGVTDR 

       970        980        990       1000       1010       1020 
VVNQLLTQLD GVEGLQGVYV LAATSRPDLI DPALLRPGRL DKCVYCPPPD QVSRLEILTV 

      1030       1040       1050       1060       1070       1080 
LSKSLALADD VDLQHVASVT DSFTGADLKA LLYNAQLEAL QGRLLPSGLP DGGSSSDSDL 

      1090       1100       1110       1120       1130       1140 
SLSSMVFLNH SSGSDDSAGD GECGLEQSLL SLEMSEILPD ESKFNMYRLY FGSSYESELG 

      1150       1160       1170       1180       1190       1200 
NGTPSDLSSH CLSAPSSVTQ DLPAAPGKDP LFTQHPVFRT PSQEGCQDLT QEQRDQLRAE 

      1210       1220       1230       1240       1250       1260 
ISIIKGRYRS QSGEDESLNQ PGPIKTTFAI SQAHLMTALA HTRPSISEDE GKEFAELYEN 

      1270       1280 
FQNPKKRKNQ SGTVFRTGQK VTLA 

« Hide

Isoform 2 [UniParc].

Checksum: 9B1073BBA4CC4106
Show »

FASTA1,244136,743

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6.
Tissue: Brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-505 AND 795-1284 (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Head and Liver.
[3]"The common phospholipid-binding activity of the N-terminal domains of PEX1 and VCP/p97."
Shiozawa K., Goda N., Shimizu T., Mizuguchi K., Kondo N., Shimozawa N., Shirakawa M., Hiroaki H.
FEBS J. 273:4959-4971(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHOLIPID BINDING, MUTAGENESIS OF ARG-135 AND LYS-174.
[4]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[5]"Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[6]"Structure of the N-terminal domain of PEX1 AAA-ATPase. Characterization of a putative adaptor-binding domain."
Shiozawa K., Maita N., Tomii K., Seto A., Goda N., Akiyama Y., Shimizu T., Shirakawa M., Hiroaki H.
J. Biol. Chem. 279:50060-50068(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 3-181.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC090845 mRNA. Translation: AAH90845.1.
AK017309 mRNA. Translation: BAB30684.1.
AK149599 mRNA. Translation: BAE28984.1.
CCDSCCDS19065.1. [Q5BL07-2]
RefSeqNP_082053.1. NM_027777.1. [Q5BL07-2]
XP_006503668.1. XM_006503605.1. [Q5BL07-1]
UniGeneMm.379196.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1WLFX-ray2.05A3-181[»]
ProteinModelPortalQ5BL07.
SMRQ5BL07. Positions 13-179.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid214674. 1 interaction.

PTM databases

PhosphoSiteQ5BL07.

Proteomic databases

MaxQBQ5BL07.
PaxDbQ5BL07.
PRIDEQ5BL07.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000006061; ENSMUSP00000006061; ENSMUSG00000005907. [Q5BL07-2]
ENSMUST00000121291; ENSMUSP00000113304; ENSMUSG00000005907. [Q5BL07-1]
GeneID71382.
KEGGmmu:71382.
UCSCuc008whf.1. mouse. [Q5BL07-2]

Organism-specific databases

CTD5189.
MGIMGI:1918632. Pex1.

Phylogenomic databases

eggNOGCOG0464.
GeneTreeENSGT00550000075032.
HOGENOMHOG000252959.
HOVERGENHBG008169.
InParanoidQ5BL07.
KOK13338.
OMAVHSWEKE.
OrthoDBEOG7W9RT0.
PhylomeDBQ5BL07.
TreeFamTF106447.

Gene expression databases

ArrayExpressQ5BL07.
BgeeQ5BL07.
CleanExMM_PEX1.
GenevestigatorQ5BL07.

Family and domain databases

Gene3D3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR029067. CDC48_domain_2-like.
IPR027417. P-loop_NTPase.
IPR015343. Peroxisome_synth_fac_1_a/b.
IPR015342. PEX-1N.
IPR025653. Pex1.
[Graphical view]
PANTHERPTHR23077:SF12. PTHR23077:SF12. 1 hit.
PfamPF00004. AAA. 2 hits.
PF09262. PEX-1N. 1 hit.
PF09263. PEX-2N. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ5BL07.
NextBio333669.
PROQ5BL07.
SOURCESearch...

Entry information

Entry namePEX1_MOUSE
AccessionPrimary (citable) accession number: Q5BL07
Secondary accession number(s): Q3UEC7, Q9CU85
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: July 9, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot