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Protein

DBIRD complex subunit ZNF326

Gene

ZNF326

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. May play a role in neuronal differentiation and is able to bind DNA and activate expression in vitro.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri314 – 336C2H2 AKAP95-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri407 – 430C2H2 AKAP95-type 2PROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • poly(A) RNA binding Source: UniProtKB
  • RNA polymerase II core binding Source: UniProtKB

GO - Biological processi

  • mRNA processing Source: UniProtKB-KW
  • regulation of DNA-templated transcription, elongation Source: UniProtKB
  • regulation of RNA splicing Source: UniProtKB
  • RNA splicing Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

mRNA processing, mRNA splicing, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DBIRD complex subunit ZNF326
Alternative name(s):
Zinc finger protein 326
Zinc finger protein interacting with mRNPs and DBC1
Gene namesi
Name:ZNF326
Synonyms:ZIRD
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:14104. ZNF326.

Subcellular locationi

GO - Cellular componenti

  • DBIRD complex Source: UniProtKB
  • intracellular membrane-bounded organelle Source: HPA
  • nuclear matrix Source: GO_Central
  • nucleoplasm Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi284695.
OpenTargetsiENSG00000162664.
PharmGKBiPA37842.

Polymorphism and mutation databases

BioMutaiZNF326.
DMDMi73622105.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000753861 – 582DBIRD complex subunit ZNF326Add BLAST582

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei48PhosphoserineCombined sources1
Modified residuei56PhosphoserineCombined sources1
Modified residuei63PhosphoserineCombined sources1
Modified residuei69PhosphoserineCombined sources1
Modified residuei81PhosphoserineCombined sources1
Modified residuei82PhosphoserineCombined sources1
Modified residuei91PhosphoserineCombined sources1
Modified residuei106PhosphoserineCombined sources1
Modified residuei114PhosphoserineCombined sources1
Modified residuei118PhosphoserineCombined sources1
Modified residuei121PhosphoserineCombined sources1
Modified residuei137PhosphoserineCombined sources1
Modified residuei173Omega-N-methylarginineCombined sources1
Modified residuei212PhosphoserineCombined sources1
Modified residuei235Omega-N-methylarginineCombined sources1
Modified residuei247N6-acetyllysineCombined sources1
Modified residuei249PhosphoserineCombined sources1
Modified residuei251PhosphothreonineCombined sources1
Modified residuei270PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ5BKZ1.
MaxQBiQ5BKZ1.
PaxDbiQ5BKZ1.
PeptideAtlasiQ5BKZ1.
PRIDEiQ5BKZ1.

PTM databases

iPTMnetiQ5BKZ1.
PhosphoSitePlusiQ5BKZ1.

Expressioni

Gene expression databases

BgeeiENSG00000162664.
CleanExiHS_ZNF326.
ExpressionAtlasiQ5BKZ1. baseline and differential.
GenevisibleiQ5BKZ1. HS.

Organism-specific databases

HPAiHPA028450.
HPA028461.
HPA028466.

Interactioni

Subunit structurei

Component of the DBIRD complex. Interacts with CCAR2; the interaction is direct.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
BRINP1O604775EBI-2560158,EBI-3904864

GO - Molecular functioni

  • RNA polymerase II core binding Source: UniProtKB

Protein-protein interaction databases

BioGridi129936. 57 interactors.
IntActiQ5BKZ1. 31 interactors.
MINTiMINT-4826604.
STRINGi9606.ENSP00000340796.

Structurei

3D structure databases

ProteinModelPortaliQ5BKZ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 124Mediates transcriptional activationBy similarityAdd BLAST124

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi238 – 260Bipartite nuclear localization signalBy similarityAdd BLAST23

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi18 – 211Gly-richAdd BLAST194
Compositional biasi484 – 581Glu-richAdd BLAST98

Sequence similaritiesi

Belongs to the AKAP95 family.PROSITE-ProRule annotation
Contains 2 C2H2 AKAP95-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri314 – 336C2H2 AKAP95-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri407 – 430C2H2 AKAP95-type 2PROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IGVY. Eukaryota.
ENOG4111H5U. LUCA.
GeneTreeiENSGT00530000063777.
HOGENOMiHOG000234349.
HOVERGENiHBG080752.
InParanoidiQ5BKZ1.
KOiK13204.
OMAiPFEIQDH.
OrthoDBiEOG091G0836.
PhylomeDBiQ5BKZ1.
TreeFamiTF105407.

Family and domain databases

InterProiIPR007071. AKAP95.
[Graphical view]
PANTHERiPTHR12190. PTHR12190. 1 hit.
PfamiPF04988. AKAP95. 1 hit.
[Graphical view]
PROSITEiPS51799. ZF_C2H2_AKAP95. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5BKZ1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDFEDDYTHS ACRNTYQGFN GMDRDYGPGS YGGMDRDYGH GSYGGQRSMD
60 70 80 90 100
SYLNQSYGMD NHSGGGGGSR FGPYESYDSR SSLGGRDLYR SGYGFNEPEQ
110 120 130 140 150
SRFGGSYGGR FESSYRNSLD SFGGRNQGGS SWEAPYSRSK LRPGFMEDRG
160 170 180 190 200
RENYSSYSSF SSPHMKPAPV GSRGRGTPAY PESTFGSRNY DAFGGPSTGR
210 220 230 240 250
GRGRGHMGDF GSIHRPGIVV DYQNKSTNVT VAAARGIKRK MMQPFNKPSG
260 270 280 290 300
TFIKKPKLAK PMEKISLSKS PTKTDPKNEE EEKRRIEARR EKQRRRREKN
310 320 330 340 350
SEKYGDGYRM AFTCSFCKFR TFEEKDIELH LESSSHQETL DHIQKQTKFD
360 370 380 390 400
KVVMEFLHEC MVNKFKKTSI RKQQTNNQTE VVKIIEKDVM EGVTVDDHMM
410 420 430 440 450
KVETVHCSAC SVYIPALHSS VQQHLKSPDH IKGKQAYKEQ IKRESVLTAT
460 470 480 490 500
SILNNPIVKA RYERFVKGEN PFEIQDHSQD QQIEGDEEDE EKIDEPIEEE
510 520 530 540 550
EDEDEEEEAE EVGEVEEVEE VEEVREGGIE GEGNIQGVGE GGEVGVVGEV
560 570 580
EGVGEVEEVE ELEEETAKEE PADFPVEQPE EN
Length:582
Mass (Da):65,654
Last modified:August 16, 2005 - v2
Checksum:i7F513DA0C5B7F3CD
GO
Isoform 2 (identifier: Q5BKZ1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     71-582: Missing.

Note: No experimental confirmation available.
Show »
Length:70
Mass (Da):7,668
Checksum:iFF2BDBA284F6BE1A
GO
Isoform 3 (identifier: Q5BKZ1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-206: Missing.

Note: No experimental confirmation available.
Show »
Length:376
Mass (Da):43,101
Checksum:iA9F9CB75B1BBEB29
GO

Sequence cautioni

The sequence AAH52645 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH70341 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence CAH72229 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAI23074 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti471P → S in AAH90866 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0437721 – 206Missing in isoform 3. 1 PublicationAdd BLAST206
Alternative sequenceiVSP_01495671 – 582Missing in isoform 2. 1 PublicationAdd BLAST512

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK297073 mRNA. Translation: BAG59592.1.
AK303707 mRNA. Translation: BAG64691.1.
AL161797, AL391497 Genomic DNA. Translation: CAI23073.1.
AL161797, AL391497 Genomic DNA. Translation: CAI23074.1. Sequence problems.
AL391497, AL161797 Genomic DNA. Translation: CAH72228.1.
AL391497, AL161797 Genomic DNA. Translation: CAH72229.1. Sequence problems.
AL391497 Genomic DNA. Translation: CAH72230.1.
CH471097 Genomic DNA. Translation: EAW73125.1.
CH471097 Genomic DNA. Translation: EAW73128.1.
BC052645 mRNA. Translation: AAH52645.1. Sequence problems.
BC053888 mRNA. Translation: AAH53888.1.
BC070341 mRNA. Translation: AAH70341.1. Sequence problems.
BC090866 mRNA. Translation: AAH90866.1.
CCDSiCCDS727.1. [Q5BKZ1-1]
CCDS728.1. [Q5BKZ1-2]
RefSeqiNP_001307114.1. NM_001320185.1.
NP_861446.2. NM_181781.3. [Q5BKZ1-3]
NP_892020.1. NM_182975.3. [Q5BKZ1-2]
NP_892021.1. NM_182976.3. [Q5BKZ1-1]
XP_005270836.1. XM_005270779.4. [Q5BKZ1-3]
XP_011539592.1. XM_011541290.2. [Q5BKZ1-3]
UniGeneiHs.306221.
Hs.586567.
Hs.732388.

Genome annotation databases

EnsembliENST00000340281; ENSP00000340796; ENSG00000162664. [Q5BKZ1-1]
ENST00000361911; ENSP00000355318; ENSG00000162664. [Q5BKZ1-2]
GeneIDi284695.
KEGGihsa:284695.
UCSCiuc001dnp.5. human. [Q5BKZ1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK297073 mRNA. Translation: BAG59592.1.
AK303707 mRNA. Translation: BAG64691.1.
AL161797, AL391497 Genomic DNA. Translation: CAI23073.1.
AL161797, AL391497 Genomic DNA. Translation: CAI23074.1. Sequence problems.
AL391497, AL161797 Genomic DNA. Translation: CAH72228.1.
AL391497, AL161797 Genomic DNA. Translation: CAH72229.1. Sequence problems.
AL391497 Genomic DNA. Translation: CAH72230.1.
CH471097 Genomic DNA. Translation: EAW73125.1.
CH471097 Genomic DNA. Translation: EAW73128.1.
BC052645 mRNA. Translation: AAH52645.1. Sequence problems.
BC053888 mRNA. Translation: AAH53888.1.
BC070341 mRNA. Translation: AAH70341.1. Sequence problems.
BC090866 mRNA. Translation: AAH90866.1.
CCDSiCCDS727.1. [Q5BKZ1-1]
CCDS728.1. [Q5BKZ1-2]
RefSeqiNP_001307114.1. NM_001320185.1.
NP_861446.2. NM_181781.3. [Q5BKZ1-3]
NP_892020.1. NM_182975.3. [Q5BKZ1-2]
NP_892021.1. NM_182976.3. [Q5BKZ1-1]
XP_005270836.1. XM_005270779.4. [Q5BKZ1-3]
XP_011539592.1. XM_011541290.2. [Q5BKZ1-3]
UniGeneiHs.306221.
Hs.586567.
Hs.732388.

3D structure databases

ProteinModelPortaliQ5BKZ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi129936. 57 interactors.
IntActiQ5BKZ1. 31 interactors.
MINTiMINT-4826604.
STRINGi9606.ENSP00000340796.

PTM databases

iPTMnetiQ5BKZ1.
PhosphoSitePlusiQ5BKZ1.

Polymorphism and mutation databases

BioMutaiZNF326.
DMDMi73622105.

Proteomic databases

EPDiQ5BKZ1.
MaxQBiQ5BKZ1.
PaxDbiQ5BKZ1.
PeptideAtlasiQ5BKZ1.
PRIDEiQ5BKZ1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000340281; ENSP00000340796; ENSG00000162664. [Q5BKZ1-1]
ENST00000361911; ENSP00000355318; ENSG00000162664. [Q5BKZ1-2]
GeneIDi284695.
KEGGihsa:284695.
UCSCiuc001dnp.5. human. [Q5BKZ1-1]

Organism-specific databases

CTDi284695.
DisGeNETi284695.
GeneCardsiZNF326.
HGNCiHGNC:14104. ZNF326.
HPAiHPA028450.
HPA028461.
HPA028466.
MIMi614601. gene.
neXtProtiNX_Q5BKZ1.
OpenTargetsiENSG00000162664.
PharmGKBiPA37842.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IGVY. Eukaryota.
ENOG4111H5U. LUCA.
GeneTreeiENSGT00530000063777.
HOGENOMiHOG000234349.
HOVERGENiHBG080752.
InParanoidiQ5BKZ1.
KOiK13204.
OMAiPFEIQDH.
OrthoDBiEOG091G0836.
PhylomeDBiQ5BKZ1.
TreeFamiTF105407.

Miscellaneous databases

ChiTaRSiZNF326. human.
GenomeRNAii284695.
PROiQ5BKZ1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000162664.
CleanExiHS_ZNF326.
ExpressionAtlasiQ5BKZ1. baseline and differential.
GenevisibleiQ5BKZ1. HS.

Family and domain databases

InterProiIPR007071. AKAP95.
[Graphical view]
PANTHERiPTHR12190. PTHR12190. 1 hit.
PfamiPF04988. AKAP95. 1 hit.
[Graphical view]
PROSITEiPS51799. ZF_C2H2_AKAP95. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZN326_HUMAN
AccessioniPrimary (citable) accession number: Q5BKZ1
Secondary accession number(s): A8MYX1
, B4DLN0, B4E179, Q5VW93, Q5VW94, Q5VW96, Q5VW97, Q6NSA2, Q7Z638, Q7Z6C2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: August 16, 2005
Last modified: November 30, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.