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Protein

Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase

Gene

ALG10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc2-PP-Dol.

Catalytic activityi

Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->2)-D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-HSA-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT59 Glycosyltransferase Family 59

Names & Taxonomyi

Protein namesi
Recommended name:
Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase (EC:2.4.1.256)
Alternative name(s):
Alpha-1,2-glucosyltransferase ALG10-A
Alpha-2-glucosyltransferase ALG10-A
Asparagine-linked glycosylation protein 10 homolog A
Gene namesi
Name:ALG10
Synonyms:ALG10A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000139133.6
HGNCiHGNC:23162 ALG10
neXtProtiNX_Q5BKT4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 27HelicalSequence analysisAdd BLAST21
Topological domaini28 – 64ExtracellularSequence analysisAdd BLAST37
Transmembranei65 – 85HelicalSequence analysisAdd BLAST21
Topological domaini86 – 97CytoplasmicSequence analysisAdd BLAST12
Transmembranei98 – 118HelicalSequence analysisAdd BLAST21
Topological domaini119 – 130ExtracellularSequence analysisAdd BLAST12
Transmembranei131 – 151HelicalSequence analysisAdd BLAST21
Transmembranei152 – 172HelicalSequence analysisAdd BLAST21
Topological domaini173 – 175ExtracellularSequence analysis3
Transmembranei176 – 196HelicalSequence analysisAdd BLAST21
Topological domaini197 – 249CytoplasmicSequence analysisAdd BLAST53
Transmembranei250 – 270HelicalSequence analysisAdd BLAST21
Topological domaini271 – 283ExtracellularSequence analysisAdd BLAST13
Transmembranei284 – 304HelicalSequence analysisAdd BLAST21
Topological domaini305 – 323CytoplasmicSequence analysisAdd BLAST19
Transmembranei324 – 344HelicalSequence analysisAdd BLAST21
Topological domaini345 – 367ExtracellularSequence analysisAdd BLAST23
Transmembranei368 – 388HelicalSequence analysisAdd BLAST21
Topological domaini389 – 392CytoplasmicSequence analysis4
Transmembranei393 – 413HelicalSequence analysisAdd BLAST21
Topological domaini414 – 436ExtracellularSequence analysisAdd BLAST23
Transmembranei437 – 457HelicalSequence analysisAdd BLAST21
Topological domaini458 – 473CytoplasmicSequence analysisAdd BLAST16

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi84920
MalaCardsiALG10
OpenTargetsiENSG00000139133
Orphaneti101016 Romano-Ward syndrome
PharmGKBiPA134732019

Polymorphism and mutation databases

BioMutaiALG10
DMDMi74736030

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002154471 – 473Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferaseAdd BLAST473

Proteomic databases

EPDiQ5BKT4
MaxQBiQ5BKT4
PaxDbiQ5BKT4
PeptideAtlasiQ5BKT4
PRIDEiQ5BKT4

PTM databases

iPTMnetiQ5BKT4
PhosphoSitePlusiQ5BKT4

Expressioni

Gene expression databases

BgeeiENSG00000139133
CleanExiHS_ALG10
ExpressionAtlasiQ5BKT4 baseline and differential
GenevisibleiQ5BKT4 HS

Organism-specific databases

HPAiHPA043329
HPA063387

Interactioni

Protein-protein interaction databases

BioGridi124356, 25 interactors
IntActiQ5BKT4, 3 interactors
STRINGi9606.ENSP00000266483

Structurei

3D structure databases

ProteinModelPortaliQ5BKT4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALG10 glucosyltransferase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2642 Eukaryota
ENOG410YGXE LUCA
GeneTreeiENSGT00390000012906
HOGENOMiHOG000240902
HOVERGENiHBG053222
InParanoidiQ5BKT4
KOiK03850
OMAiWWFRYAM
OrthoDBiEOG091G0A27
PhylomeDBiQ5BKT4
TreeFamiTF300150

Family and domain databases

InterProiView protein in InterPro
IPR016900 Alg10
PANTHERiPTHR12989 PTHR12989, 1 hit
PfamiView protein in Pfam
PF04922 DIE2_ALG10, 1 hit
PIRSFiPIRSF028810 Alpha1_2_glucosyltferase_Alg10, 1 hit

Sequencei

Sequence statusi: Complete.

Q5BKT4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQLEGYYFS AALSCTFLVS CLLFSAFSRA LREPYMDEIF HLPQAQRYCE
60 70 80 90 100
GHFSLSQWDP MITTLPGLYL VSIGVIKPAI WIFGWSEHVV CSIGMLRFVN
110 120 130 140 150
LLFSVGNFYL LYLLFCKVQP RNKAASSIQR VLSTLTLAVF PTLYFFNFLY
160 170 180 190 200
YTEAGSMFFT LFAYLMCLYG NHKTSAFLGF CGFMFRQTNI IWAVFCAGNV
210 220 230 240 250
IAQKLTEAWK TELQKKEDRL PPIKGPFAEF RKILQFLLAY SMSFKNLSML
260 270 280 290 300
LLLTWPYILL GFLFCAFVVV NGGIVIGDRS SHEACLHFPQ LFYFFSFTLF
310 320 330 340 350
FSFPHLLSPS KIKTFLSLVW KRRILFFVVT LVSVFLVWKF TYAHKYLLAD
360 370 380 390 400
NRHYTFYVWK RVFQRYETVK YLLVPAYIFA GWSIADSLKS KSIFWNLMFF
410 420 430 440 450
ICLFTVIVPQ KLLEFRYFIL PYVIYRLNIP LPPTSRLICE LSCYAVVNFI
460 470
TFFIFLNKTF QWPNSQDIQR FMW
Length:473
Mass (Da):55,606
Last modified:April 12, 2005 - v1
Checksum:i1860D4E61ACBC2A0
GO

Sequence cautioni

The sequence BAB55272 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti184M → V in AAH70347 (PubMed:15489334).Curated1
Sequence conflicti258I → T in CAC41349 (PubMed:12200473).Curated1
Sequence conflicti384I → T in BAB55272 (PubMed:14702039).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ312278 mRNA Translation: CAC41349.1
BC070347 mRNA Translation: AAH70347.1
BC090948 mRNA Translation: AAH90948.1
AK027657 mRNA Translation: BAB55272.1 Different initiation.
CCDSiCCDS41769.1
RefSeqiNP_116223.3, NM_032834.3
UniGeneiHs.102971

Genome annotation databases

EnsembliENST00000266483; ENSP00000266483; ENSG00000139133
GeneIDi84920
KEGGihsa:84920
UCSCiuc001rlm.4 human

Similar proteinsi

Entry informationi

Entry nameiAG10A_HUMAN
AccessioniPrimary (citable) accession number: Q5BKT4
Secondary accession number(s): Q6NS98, Q96DU0, Q96SM6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: April 12, 2005
Last modified: April 25, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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