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Q5BKT4

- AG10A_HUMAN

UniProt

Q5BKT4 - AG10A_HUMAN

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Protein

Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase

Gene
ALG10, ALG10A
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol.

Catalytic activityi

Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->2)-D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathwayi

GO - Molecular functioni

  1. transferase activity, transferring hexosyl groups Source: InterPro

GO - Biological processi

  1. cellular protein metabolic process Source: Reactome
  2. dolichol-linked oligosaccharide biosynthetic process Source: Reactome
  3. post-translational protein modification Source: Reactome
  4. protein N-linked glycosylation via asparagine Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_22433. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT59. Glycosyltransferase Family 59.

Names & Taxonomyi

Protein namesi
Recommended name:
Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase (EC:2.4.1.256)
Alternative name(s):
Alpha-1,2-glucosyltransferase ALG10-A
Alpha-2-glucosyltransferase ALG10-A
Asparagine-linked glycosylation protein 10 homolog A
Gene namesi
Name:ALG10
Synonyms:ALG10A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 12

Organism-specific databases

HGNCiHGNC:23162. ALG10.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66Cytoplasmic Reviewed prediction
Transmembranei7 – 2721Helical; Reviewed predictionAdd
BLAST
Topological domaini28 – 6437Extracellular Reviewed predictionAdd
BLAST
Transmembranei65 – 8521Helical; Reviewed predictionAdd
BLAST
Topological domaini86 – 9712Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei98 – 11821Helical; Reviewed predictionAdd
BLAST
Topological domaini119 – 13012Extracellular Reviewed predictionAdd
BLAST
Transmembranei131 – 15121Helical; Reviewed predictionAdd
BLAST
Transmembranei152 – 17221Helical; Reviewed predictionAdd
BLAST
Topological domaini173 – 1753Extracellular Reviewed prediction
Transmembranei176 – 19621Helical; Reviewed predictionAdd
BLAST
Topological domaini197 – 24953Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei250 – 27021Helical; Reviewed predictionAdd
BLAST
Topological domaini271 – 28313Extracellular Reviewed predictionAdd
BLAST
Transmembranei284 – 30421Helical; Reviewed predictionAdd
BLAST
Topological domaini305 – 32319Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei324 – 34421Helical; Reviewed predictionAdd
BLAST
Topological domaini345 – 36723Extracellular Reviewed predictionAdd
BLAST
Transmembranei368 – 38821Helical; Reviewed predictionAdd
BLAST
Topological domaini389 – 3924Cytoplasmic Reviewed prediction
Transmembranei393 – 41321Helical; Reviewed predictionAdd
BLAST
Topological domaini414 – 43623Extracellular Reviewed predictionAdd
BLAST
Transmembranei437 – 45721Helical; Reviewed predictionAdd
BLAST
Topological domaini458 – 47316Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

Orphaneti101016. Romano-Ward syndrome.
PharmGKBiPA134732019.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 473473Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferasePRO_0000215447Add
BLAST

Proteomic databases

MaxQBiQ5BKT4.
PaxDbiQ5BKT4.
PRIDEiQ5BKT4.

PTM databases

PhosphoSiteiQ5BKT4.

Expressioni

Gene expression databases

ArrayExpressiQ5BKT4.
BgeeiQ5BKT4.
CleanExiHS_ALG10.
GenevestigatoriQ5BKT4.

Organism-specific databases

HPAiHPA043329.

Interactioni

Protein-protein interaction databases

BioGridi124356. 1 interaction.
STRINGi9606.ENSP00000266483.

Structurei

3D structure databases

ProteinModelPortaliQ5BKT4.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG236252.
HOGENOMiHOG000240902.
HOVERGENiHBG053222.
InParanoidiQ5BKT4.
KOiK03850.
OMAiFLLTWPY.
OrthoDBiEOG76HQ16.
PhylomeDBiQ5BKT4.
TreeFamiTF300150.

Family and domain databases

InterProiIPR016900. Alg10.
[Graphical view]
PANTHERiPTHR12989. PTHR12989. 1 hit.
PfamiPF04922. DIE2_ALG10. 1 hit.
[Graphical view]
PIRSFiPIRSF028810. Alpha1_2_glucosyltferase_Alg10. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5BKT4-1 [UniParc]FASTAAdd to Basket

« Hide

MAQLEGYYFS AALSCTFLVS CLLFSAFSRA LREPYMDEIF HLPQAQRYCE    50
GHFSLSQWDP MITTLPGLYL VSIGVIKPAI WIFGWSEHVV CSIGMLRFVN 100
LLFSVGNFYL LYLLFCKVQP RNKAASSIQR VLSTLTLAVF PTLYFFNFLY 150
YTEAGSMFFT LFAYLMCLYG NHKTSAFLGF CGFMFRQTNI IWAVFCAGNV 200
IAQKLTEAWK TELQKKEDRL PPIKGPFAEF RKILQFLLAY SMSFKNLSML 250
LLLTWPYILL GFLFCAFVVV NGGIVIGDRS SHEACLHFPQ LFYFFSFTLF 300
FSFPHLLSPS KIKTFLSLVW KRRILFFVVT LVSVFLVWKF TYAHKYLLAD 350
NRHYTFYVWK RVFQRYETVK YLLVPAYIFA GWSIADSLKS KSIFWNLMFF 400
ICLFTVIVPQ KLLEFRYFIL PYVIYRLNIP LPPTSRLICE LSCYAVVNFI 450
TFFIFLNKTF QWPNSQDIQR FMW 473
Length:473
Mass (Da):55,606
Last modified:April 12, 2005 - v1
Checksum:i1860D4E61ACBC2A0
GO

Sequence cautioni

The sequence BAB55272.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti184 – 1841M → V in AAH70347. 1 Publication
Sequence conflicti258 – 2581I → T in CAC41349. 1 Publication
Sequence conflicti384 – 3841I → T in BAB55272. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ312278 mRNA. Translation: CAC41349.1.
BC070347 mRNA. Translation: AAH70347.1.
BC090948 mRNA. Translation: AAH90948.1.
AK027657 mRNA. Translation: BAB55272.1. Different initiation.
CCDSiCCDS41769.1.
RefSeqiNP_116223.3. NM_032834.3.
UniGeneiHs.102971.

Genome annotation databases

EnsembliENST00000266483; ENSP00000266483; ENSG00000139133.
GeneIDi84920.
KEGGihsa:84920.
UCSCiuc001rlm.3. human.

Polymorphism databases

DMDMi74736030.

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ312278 mRNA. Translation: CAC41349.1 .
BC070347 mRNA. Translation: AAH70347.1 .
BC090948 mRNA. Translation: AAH90948.1 .
AK027657 mRNA. Translation: BAB55272.1 . Different initiation.
CCDSi CCDS41769.1.
RefSeqi NP_116223.3. NM_032834.3.
UniGenei Hs.102971.

3D structure databases

ProteinModelPortali Q5BKT4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 124356. 1 interaction.
STRINGi 9606.ENSP00000266483.

Protein family/group databases

CAZyi GT59. Glycosyltransferase Family 59.

PTM databases

PhosphoSitei Q5BKT4.

Polymorphism databases

DMDMi 74736030.

Proteomic databases

MaxQBi Q5BKT4.
PaxDbi Q5BKT4.
PRIDEi Q5BKT4.

Protocols and materials databases

DNASUi 84920.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000266483 ; ENSP00000266483 ; ENSG00000139133 .
GeneIDi 84920.
KEGGi hsa:84920.
UCSCi uc001rlm.3. human.

Organism-specific databases

CTDi 84920.
GeneCardsi GC12P034075.
H-InvDB HIX0036775.
HGNCi HGNC:23162. ALG10.
HPAi HPA043329.
neXtProti NX_Q5BKT4.
Orphaneti 101016. Romano-Ward syndrome.
PharmGKBi PA134732019.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG236252.
HOGENOMi HOG000240902.
HOVERGENi HBG053222.
InParanoidi Q5BKT4.
KOi K03850.
OMAi FLLTWPY.
OrthoDBi EOG76HQ16.
PhylomeDBi Q5BKT4.
TreeFami TF300150.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_22433. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

GenomeRNAii 84920.
NextBioi 75328.
PROi Q5BKT4.

Gene expression databases

ArrayExpressi Q5BKT4.
Bgeei Q5BKT4.
CleanExi HS_ALG10.
Genevestigatori Q5BKT4.

Family and domain databases

InterProi IPR016900. Alg10.
[Graphical view ]
PANTHERi PTHR12989. PTHR12989. 1 hit.
Pfami PF04922. DIE2_ALG10. 1 hit.
[Graphical view ]
PIRSFi PIRSF028810. Alpha1_2_glucosyltferase_Alg10. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Common origin and evolution of glycosyltransferases using Dol-P-monosaccharides as donor substrate."
    Oriol R., Martinez-Duncker I., Chantret I., Mollicone R., Codogno P.
    Mol. Biol. Evol. 19:1451-1463(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Embryo.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain and Lung.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 149-473.

Entry informationi

Entry nameiAG10A_HUMAN
AccessioniPrimary (citable) accession number: Q5BKT4
Secondary accession number(s): Q6NS98, Q96DU0, Q96SM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: April 12, 2005
Last modified: September 3, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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