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Q5BKT4

- AG10A_HUMAN

UniProt

Q5BKT4 - AG10A_HUMAN

Protein

Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase

Gene

ALG10

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (12 Apr 2005)
      Previous versions | rss
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    Functioni

    Adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol.

    Catalytic activityi

    Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->2)-D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

    Pathwayi

    GO - Molecular functioni

    1. transferase activity, transferring hexosyl groups Source: InterPro

    GO - Biological processi

    1. cellular protein metabolic process Source: Reactome
    2. dolichol-linked oligosaccharide biosynthetic process Source: Reactome
    3. post-translational protein modification Source: Reactome
    4. protein N-linked glycosylation via asparagine Source: Reactome

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    ReactomeiREACT_22433. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT59. Glycosyltransferase Family 59.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase (EC:2.4.1.256)
    Alternative name(s):
    Alpha-1,2-glucosyltransferase ALG10-A
    Alpha-2-glucosyltransferase ALG10-A
    Asparagine-linked glycosylation protein 10 homolog A
    Gene namesi
    Name:ALG10
    Synonyms:ALG10A
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:23162. ALG10.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Organism-specific databases

    Orphaneti101016. Romano-Ward syndrome.
    PharmGKBiPA134732019.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 473473Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferasePRO_0000215447Add
    BLAST

    Proteomic databases

    MaxQBiQ5BKT4.
    PaxDbiQ5BKT4.
    PRIDEiQ5BKT4.

    PTM databases

    PhosphoSiteiQ5BKT4.

    Expressioni

    Gene expression databases

    ArrayExpressiQ5BKT4.
    BgeeiQ5BKT4.
    CleanExiHS_ALG10.
    GenevestigatoriQ5BKT4.

    Organism-specific databases

    HPAiHPA043329.

    Interactioni

    Protein-protein interaction databases

    BioGridi124356. 1 interaction.
    STRINGi9606.ENSP00000266483.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5BKT4.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 66CytoplasmicSequence Analysis
    Topological domaini28 – 6437ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini86 – 9712CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini119 – 13012ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini173 – 1753ExtracellularSequence Analysis
    Topological domaini197 – 24953CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini271 – 28313ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini305 – 32319CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini345 – 36723ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini389 – 3924CytoplasmicSequence Analysis
    Topological domaini414 – 43623ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini458 – 47316CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei65 – 8521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei98 – 11821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei131 – 15121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei152 – 17221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei176 – 19621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei250 – 27021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei284 – 30421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei324 – 34421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei368 – 38821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei393 – 41321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei437 – 45721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ALG10 glucosyltransferase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG236252.
    HOGENOMiHOG000240902.
    HOVERGENiHBG053222.
    InParanoidiQ5BKT4.
    KOiK03850.
    OMAiFLLTWPY.
    OrthoDBiEOG76HQ16.
    PhylomeDBiQ5BKT4.
    TreeFamiTF300150.

    Family and domain databases

    InterProiIPR016900. Alg10.
    [Graphical view]
    PANTHERiPTHR12989. PTHR12989. 1 hit.
    PfamiPF04922. DIE2_ALG10. 1 hit.
    [Graphical view]
    PIRSFiPIRSF028810. Alpha1_2_glucosyltferase_Alg10. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5BKT4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQLEGYYFS AALSCTFLVS CLLFSAFSRA LREPYMDEIF HLPQAQRYCE    50
    GHFSLSQWDP MITTLPGLYL VSIGVIKPAI WIFGWSEHVV CSIGMLRFVN 100
    LLFSVGNFYL LYLLFCKVQP RNKAASSIQR VLSTLTLAVF PTLYFFNFLY 150
    YTEAGSMFFT LFAYLMCLYG NHKTSAFLGF CGFMFRQTNI IWAVFCAGNV 200
    IAQKLTEAWK TELQKKEDRL PPIKGPFAEF RKILQFLLAY SMSFKNLSML 250
    LLLTWPYILL GFLFCAFVVV NGGIVIGDRS SHEACLHFPQ LFYFFSFTLF 300
    FSFPHLLSPS KIKTFLSLVW KRRILFFVVT LVSVFLVWKF TYAHKYLLAD 350
    NRHYTFYVWK RVFQRYETVK YLLVPAYIFA GWSIADSLKS KSIFWNLMFF 400
    ICLFTVIVPQ KLLEFRYFIL PYVIYRLNIP LPPTSRLICE LSCYAVVNFI 450
    TFFIFLNKTF QWPNSQDIQR FMW 473
    Length:473
    Mass (Da):55,606
    Last modified:April 12, 2005 - v1
    Checksum:i1860D4E61ACBC2A0
    GO

    Sequence cautioni

    The sequence BAB55272.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti184 – 1841M → V in AAH70347. (PubMed:15489334)Curated
    Sequence conflicti258 – 2581I → T in CAC41349. (PubMed:12200473)Curated
    Sequence conflicti384 – 3841I → T in BAB55272. (PubMed:14702039)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ312278 mRNA. Translation: CAC41349.1.
    BC070347 mRNA. Translation: AAH70347.1.
    BC090948 mRNA. Translation: AAH90948.1.
    AK027657 mRNA. Translation: BAB55272.1. Different initiation.
    CCDSiCCDS41769.1.
    RefSeqiNP_116223.3. NM_032834.3.
    UniGeneiHs.102971.

    Genome annotation databases

    EnsembliENST00000266483; ENSP00000266483; ENSG00000139133.
    GeneIDi84920.
    KEGGihsa:84920.
    UCSCiuc001rlm.3. human.

    Polymorphism databases

    DMDMi74736030.

    Cross-referencesi

    Web resourcesi

    GGDB

    GlycoGene database

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ312278 mRNA. Translation: CAC41349.1 .
    BC070347 mRNA. Translation: AAH70347.1 .
    BC090948 mRNA. Translation: AAH90948.1 .
    AK027657 mRNA. Translation: BAB55272.1 . Different initiation.
    CCDSi CCDS41769.1.
    RefSeqi NP_116223.3. NM_032834.3.
    UniGenei Hs.102971.

    3D structure databases

    ProteinModelPortali Q5BKT4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 124356. 1 interaction.
    STRINGi 9606.ENSP00000266483.

    Protein family/group databases

    CAZyi GT59. Glycosyltransferase Family 59.

    PTM databases

    PhosphoSitei Q5BKT4.

    Polymorphism databases

    DMDMi 74736030.

    Proteomic databases

    MaxQBi Q5BKT4.
    PaxDbi Q5BKT4.
    PRIDEi Q5BKT4.

    Protocols and materials databases

    DNASUi 84920.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000266483 ; ENSP00000266483 ; ENSG00000139133 .
    GeneIDi 84920.
    KEGGi hsa:84920.
    UCSCi uc001rlm.3. human.

    Organism-specific databases

    CTDi 84920.
    GeneCardsi GC12P034075.
    H-InvDB HIX0036775.
    HGNCi HGNC:23162. ALG10.
    HPAi HPA043329.
    neXtProti NX_Q5BKT4.
    Orphaneti 101016. Romano-Ward syndrome.
    PharmGKBi PA134732019.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG236252.
    HOGENOMi HOG000240902.
    HOVERGENi HBG053222.
    InParanoidi Q5BKT4.
    KOi K03850.
    OMAi FLLTWPY.
    OrthoDBi EOG76HQ16.
    PhylomeDBi Q5BKT4.
    TreeFami TF300150.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_22433. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

    Miscellaneous databases

    GenomeRNAii 84920.
    NextBioi 75328.
    PROi Q5BKT4.

    Gene expression databases

    ArrayExpressi Q5BKT4.
    Bgeei Q5BKT4.
    CleanExi HS_ALG10.
    Genevestigatori Q5BKT4.

    Family and domain databases

    InterProi IPR016900. Alg10.
    [Graphical view ]
    PANTHERi PTHR12989. PTHR12989. 1 hit.
    Pfami PF04922. DIE2_ALG10. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF028810. Alpha1_2_glucosyltferase_Alg10. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Common origin and evolution of glycosyltransferases using Dol-P-monosaccharides as donor substrate."
      Oriol R., Martinez-Duncker I., Chantret I., Mollicone R., Codogno P.
      Mol. Biol. Evol. 19:1451-1463(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Embryo.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain and Lung.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 149-473.

    Entry informationi

    Entry nameiAG10A_HUMAN
    AccessioniPrimary (citable) accession number: Q5BKT4
    Secondary accession number(s): Q6NS98, Q96DU0, Q96SM6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: April 12, 2005
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3